Human glutamic acid decarboxylase 65,GAD65 ELISA Kit

Code CSB-E14119h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q05329
Lead Time 3-5 working days
Abbreviation GAD2
Protein Biological Process 1 Neurobiology
Target Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Alias RP11-420F12.2, GAD65, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2
Species Homo sapiens (Human)
Protein Biological Process 3 Neurotransmitter biosynthesis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
HGNC 4093
RGD 2653
MGI 95634
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GAD65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %83-95
1:2Average %85
Range %81-89
1:4Average %90
Range %85-96
1:8Average %92
Range %88-97
Recovery
The recovery of human GAD65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10094-105
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.460 2.489 2.475 2.298
51.868 1.898 1.883 1.706
2.51.326 1.366 1.346 1.169
1.250.853 0.879 0.866 0.689
0.6250.533 0.574 0.554 0.377
0.3120.404 0.436 0.420 0.243
0.1560.319 0.345 0.332 0.155
00.178 0.176 0.177  
References
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Function Catalyzes the production of GABA.
Subcellular Location Cytoplasm, cytosol, Cytoplasmic vesicle, Cell junction, synapse, presynaptic cell membrane, Lipid-anchor, Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Group II decarboxylase family
Database Links

HGNC: 4093

OMIM: 138275

KEGG: hsa:2572

STRING: 9606.ENSP00000259271

UniGene: Hs.231829

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