Code | CSB-E08617h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human hs-CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:200 | Average % | 87 | ||||
Range % | 82-94 | |||||
1:400 | Average % | 93 | ||||
Range % | 89-98 | |||||
1:800 | Average % | 99 | ||||
Range % | 92-106 | |||||
1:1600 | Average % | 94 | ||||
Range % | 89-100 |
The recovery of human hs-CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 85-96 | ||||
EDTA plasma (n=4) | 95 | 90-100 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
50 | 3.077 | 2.934 | 3.006 | 2.899 | |||
25 | 2.399 | 2.267 | 2.333 | 2.226 | |||
12.5 | 1.734 | 1.728 | 1.731 | 1.624 | |||
6.25 | 1.056 | 1.029 | 1.043 | 0.936 | |||
3.12 | 0.726 | 0.711 | 0.719 | 0.612 | |||
1.56 | 0.469 | 0.453 | 0.461 | 0.354 | |||
0.78 | 0.291 | 0.284 | 0.288 | 0.181 | |||
0 | 0.109 | 0.105 | 0.107 |
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