Human insulin-like growth factors 2 receptor,IGF-2R ELISA Kit

Code CSB-E11267h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P11717
Lead Time 3-5 working days
Abbreviation IGF2R
Protein Biological Process 1 Transport
Target Name insulin-like growth factor 2 receptor
Alias CD222, CIMPR, M6P-R, MPR1, MPRI, cation-independent mannose-6 phosphate receptor
Species Homo sapiens (Human)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates
Detection Range 312.5 pg/mL-20000 pg/mL
Sensitivity 78.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Target Details This protein is a receptor for both, insulin-like growth factor 2 (IGF2) and mannose 6-phosphate (M6P). The IGF2 and M6P binding sites are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of IGF2. In mice, the homologous gene is expressed only from the maternal chromosome.
HGNC 5467
RGD 2871
MGI 96435
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-103
1:2Average %90
Range %83-96
1:4Average %102
Range %99-105
1:8Average %86
Range %81-92
Recovery
The recovery of human IGF-2R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)8982-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
200002.821 2.752 2.787 2.666
100002.420 2.279 2.350 2.229
50001.764 1.789 1.777 1.656
25000.941 1.002 0.972 0.851
12500.527 0.555 0.541 0.420
6250.318 0.346 0.332 0.211
312.50.214 0.225 0.220 0.099
00.126 0.116 0.121  
References
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Function Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Subcellular Location Lysosome membrane, Single-pass type I membrane protein
Protein Families MRL1/IGF2R family
Database Links

HGNC: 5467

OMIM: 147280

KEGG: hsa:3482

STRING: 9606.ENSP00000349437

UniGene: Hs.487062

Pathway Endocytosis

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