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Human leucocyte antigen B27,HLA-B27 ELISA Kit

Instructions
Code CSB-E08607h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leucocyte antigen B27,HLA-B27
Abbreviation HLA-B27
Uniprot No. P03989
Species Homo sapiens (Human)
Sample Types serum, urine, cerebrospinal fluid (CSF)
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 89  
Range % 80-98  
1:2 Average % 86  
Range % 80-96  
1:4 Average % 96  
Range % 92-100  
1:8 Average % 100  
Range % 96-110  
Recovery
The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.730 2.612 2.671 2.563  
10 2.361 2.241 2.301 2.193  
5 1.702 1.638 1.670 1.562  
2.5 0.933 0.871 0.902 0.794  
1.25 0.534 0.522 0.528 0.420  
0.625 0.324 0.318 0.321 0.213  
0.312 0.243 0.236 0.240 0.132  
0 0.109 0.107 0.108 　  
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Involved in the presentation of foreign antigens to the immune system.
Involvement in disease Spondyloarthropathy 1 (SPDA1)
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families MHC class I family
Database Links

HGNC: 4932

OMIM: 106300

UniGene: Hs.654404
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