Target Name |
leucocyte antigen B27,HLA-B27 |
Abbreviation |
HLA-B27 |
Uniprot No. |
P03989 |
Species |
Homo sapiens (Human) |
Sample Types |
serum, urine, cerebrospinal fluid (CSF) |
Detection Range |
0.312 ng/mL-20 ng/mL |
Sensitivity |
0.078 ng/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Others
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Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:1 |
Average % |
89 |
|
Range % |
80-98 |
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1:2 |
Average % |
86 |
|
Range % |
80-96 |
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1:4 |
Average % |
96 |
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Range % |
92-100 |
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1:8 |
Average % |
100 |
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Range % |
96-110 |
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Recovery |
The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
94 |
89-98 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
20 |
2.730 |
2.612 |
2.671 |
2.563 |
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10 |
2.361 |
2.241 |
2.301 |
2.193 |
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5 |
1.702 |
1.638 |
1.670 |
1.562 |
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2.5 |
0.933 |
0.871 |
0.902 |
0.794 |
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1.25 |
0.534 |
0.522 |
0.528 |
0.420 |
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0.625 |
0.324 |
0.318 |
0.321 |
0.213 |
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0.312 |
0.243 |
0.236 |
0.240 |
0.132 |
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0 |
0.109 |
0.107 |
0.108 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |