Human leucocyte antigen B27,HLA-B27 ELISA Kit

Code CSB-E08607h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
leucocyte antigen B27,HLA-B27
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, urine, cerebrospinal fluid (CSF)
Detection Range
0.312 ng/mL-20 ng/mL
0.078 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 80-98  
1:2 Average % 86  
Range % 80-96  
1:4 Average % 96  
Range % 92-100  
1:8 Average % 100  
Range % 96-110  
The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.730 2.612 2.671 2.563  
10 2.361 2.241 2.301 2.193  
5 1.702 1.638 1.670 1.562  
2.5 0.933 0.871 0.902 0.794  
1.25 0.534 0.522 0.528 0.420  
0.625 0.324 0.318 0.321 0.213  
0.312 0.243 0.236 0.240 0.132  
0 0.109 0.107 0.108    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human HLA-B27 ELISA Kit was designed for the quantitative measurement of Human HLA-B27 protein in serum, urine, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Involvement in disease
Spondyloarthropathy 1 (SPDA1)
Protein Families
MHC class I family
Database Links

HGNC: 4932

OMIM: 106300

UniGene: Hs.654404

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