Human leucocyte antigen B27,HLA-B27 ELISA Kit

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Product Details

Target Name

leucocyte antigen B27,HLA-B27

Abbreviation

HLA-B27

Uniprot No.

P03989

Species

Homo sapiens (Human)

Sample Types

serum, urine, cerebrospinal fluid (CSF)

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-98
1:2	Average %	86
	Range %	80-96
1:4	Average %	96
	Range %	92-100
1:8	Average %	100
	Range %	96-110

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.730	2.612	2.671	2.563
10	2.361	2.241	2.301	2.193
5	1.702	1.638	1.670	1.562
2.5	0.933	0.871	0.902	0.794
1.25	0.534	0.522	0.528	0.420
0.625	0.324	0.318	0.321	0.213
0.312	0.243	0.236	0.240	0.132
0	0.109	0.107	0.108

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Target Data

Function	Involved in the presentation of foreign antigens to the immune system.
Involvement in disease	Spondyloarthropathy 1 (SPDA1)
Subcellular Location	Membrane, Single-pass type I membrane protein
Protein Families	MHC class I family
Database Links	HGNC: 4932 OMIM: 106300 UniGene: Hs.654404

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Check Code：

Code	CSB-E08607h
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.