Product Details

Target Name

chemokine (C-C motif) ligand 15

Alternative Names

C-C motif chemokine 15 ELISA Kit; C-C motif chemokine ligand 15 ELISA Kit; CC chemokine 3 ELISA Kit; CCL15 ELISA Kit; CCL15(29-92) ELISA Kit; CCL15_HUMAN ELISA Kit; Chemokine (C C motif) ligand 15 ELISA Kit; Chemokine CC-2 ELISA Kit; Chemokine CC2 ELISA Kit; HCC-2 ELISA Kit; HCC2 ELISA Kit; HMRP 2B ELISA Kit; HMRP2B ELISA Kit; Leukotactin 1 ELISA Kit; Leukotactin-1 ELISA Kit; LKN-1 ELISA Kit; LKN1 ELISA Kit; Macrophage inflammatory protein 5 ELISA Kit; MIP 1D ELISA Kit; MIP-1 delta ELISA Kit; MIP-1D ELISA Kit; MIP-5 ELISA Kit; MIP1 delta ELISA Kit; MIP1d ELISA Kit; MIP5 ELISA Kit; MRP 2B ELISA Kit; Mrp-2b ELISA Kit; Mrp2b ELISA Kit; NCC 3 ELISA Kit; NCC-3 ELISA Kit; NCC3 ELISA Kit; New CC chemokine 3 ELISA Kit; OTTHUMP00000163955 ELISA Kit; SCYA15 ELISA Kit; SCYL3 ELISA Kit; Small inducible cytokine A15 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 15 ELISA Kit; Small-inducible cytokine A15 ELISA Kit; SY15 ELISA Kit

Abbreviation

CCL15

Uniprot No.

Q16663

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	244.575	250.280	257.432	237.463	249.328	256.000
SD	0.024	0.031	0.037	0.026	0.035	0.041
CV(%)	3.352	4.258	4.980	3.709	4.821	5.541

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:4

Average %

Range %

81-89

1:8

Average %

Range %

91-99

1:16

Average %

Range %

83-92

1:32

Average %

105

Range %

101-109

Recovery

The recovery of human MIP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
Heparin plasma (n=4)	91	89-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.652	2.701	2.677	2.505
1000	1.945	1.932	1.939	1.767
500	1.213	1.204	1.209	1.037
250	0.741	0.735	0.738	0.566
125	0.451	0.432	0.442	0.270
62.5	0.330	0.342	0.336	0.164
31.25	0.266	0.257	0.262	0.090
0	0.170	0.173	0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human CCL15 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL15 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL15 levels in the sample and is measured at 450 nm through a microplate reader.

CCL15 is a potent chemoattractant for leukocytes and endothelial cells (ECs). CCL15 exerts its role through binding to CCR1 and CCR3. It induces chemotactic migration of monocytes, dendritic cells, T lymphocytes, and eosinophils and is primarily expressed in the colon and liver. CCL15 also induces the expression of proinflammatory cytokines and tissue factors in activated monocytes. A study has demonstrated that CCL15 induces expression of ICAM-1 via activation of the JAK2/STAT3 signal pathway. CCL15 plays a role in the pathogenesis of cancer and other diseases, such as colorectal cancer, hepatocellular carcinoma, atherosclerosis, asthma, and rheumatoid arthritis.

Target Background

Function
(From Uniprot)

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.

Gene References into Functions

CCL15 secreted from SMAD4-deficient xenografted human colorectal cancer cells recruited CCR1(+) cells, promoting their metastatic activities to the lung. Lung metastases from colorectal cancer patients revealed that CCL15 expression correlated with loss of SMAD4. CCL15-positive metastases recruited approximately 1.9 times more numbers of CCR1(+) cells than CCL15-negative metastases. PMID: 27492974
CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis. PMID: 26501423
Orthotopic animal models of HCC were established to investigate the role of CCL15 in hMSCs migration toward HCC in vivo. Both histological and flow cytometric analysis showed that significantly fewer hMSCs localized within 97H-CCL15-shRNA xenografts compared with 97H-green fluorescent protein xenografts after intravenous delivery PMID: 26763650
Loss of SMAD4 was significantly associated with CCL15 expression. PMID: 26341919
follicular thyroid carcinoma (FTC) might induce tumor associated macrophages (TAMs) infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from follicular adenoma PMID: 26875556
two inflammatory cytokines, MIP-1delta and MIP-3alpha, are able to increase mesenchymal stromal cells migration in vitro PMID: 25579056
we investigated a role of CCL15 in upregulating ICAM-1 in endothelial cells PMID: 23690481
CCL15 is elevated in human asthma and correlates with disease severity . PMID: 22092970
Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551
increased secretion by macrophages during Mycobacterium tuberculosis infection PMID: 20016943
truncation of NH2-terminal amino acid residues increases agonistic potency on CC chemokine receptors 1 and 3 PMID: 11832479
Lkn-1 activates the ERK pathway by transducing the signal through G(i)/G(o) protein, PLC, PKC delta and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes PMID: 12759139
These observations demonstrate that the two NF-kappaB binding sites are essential for phorbol myristate acetate-induced leukotactin-1 (Lkn-1)/CCL15 expression in human monocytes. PMID: 15179048
CCL15(25-92) has in vitro and in vivo angiogenic activity PMID: 15251437
alanine-aspartic acid residues preceding the first cysteine at the NH(2)-terminus are essential for the binding and biological activity of leukotactin-1 PMID: 15894113
Compared with full-length CCL15, proteolytically processed CCL15 isoforms with N-terminal deletions display increased potency to induce calcium fluxes and chemotactic activity on monocytes and to induce adhesiveness of mononuclear cells to fibronectin. PMID: 16034099
Transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells. PMID: 16364464
Results point to an involvement of the CCL15-CCR1 axis in the pathophysiology of chronic renal failure. PMID: 16737685
factor NF-kappaB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1 PMID: 17296613

Hide All

Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.

Database Links

HGNC: 10613

OMIM: 601393

KEGG: hsa:6359

STRING: 9606.ENSP00000432034

UniGene: Hs.272493

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Code	CSB-E07997h
Size	96T,5×96T,10×96T
Price	Request a Quote
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