Name: Recombinant Human C-C motif chemokine 15 protein(CCL15) (Active)
Brand: CUSABIO
SKU: CSB-AP000581HU

Product Details

Purity	>98% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
Target Names	CCL15
Uniprot No.	Q16663
Research Area	Immunology
Alternative Names	C-C motif chemokine 15; C-C motif chemokine ligand 15; CC chemokine 3; CCL15; CCL15(29-92); CCL15_HUMAN; Chemokine (C C motif) ligand 15; Chemokine CC-2; Chemokine CC2; HCC-2; HCC2; HMRP 2B; HMRP2B; Leukotactin 1; Leukotactin-1; LKN-1; LKN1; Macrophage inflammatory protein 5; MIP 1D; MIP-1 delta; MIP-1D; MIP-5; MIP1 delta; MIP1d; MIP5; MRP 2B; Mrp-2b; Mrp2b; NCC 3; NCC-3; NCC3; New CC chemokine 3; OTTHUMP00000163955; SCYA15; SCYL3; Small inducible cytokine A15; Small inducible cytokine subfamily A (Cys Cys); member 15; Small-inducible cytokine A15; SY15
Species	Homo sapiens (Human)
Source	E.coli
Expression Region	46-113aa
Complete Sequence	SFHFAADCCT SYISQSIPCS LMKSYFETSS ECSKPGVIFL TKKGRQVCAK PSGPGVQDCM KKLKPYSI
Mol. Weight	7.4 kDa
Protein Length	Partial
Tag Info	Tag-Free
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	5-10 business days
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function	Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
Gene References into Functions	CCL15 secreted from SMAD4-deficient xenografted human colorectal cancer cells recruited CCR1(+) cells, promoting their metastatic activities to the lung. Lung metastases from colorectal cancer patients revealed that CCL15 expression correlated with loss of SMAD4. CCL15-positive metastases recruited approximately 1.9 times more numbers of CCR1(+) cells than CCL15-negative metastases. PMID: 27492974 CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis. PMID: 26501423 Orthotopic animal models of HCC were established to investigate the role of CCL15 in hMSCs migration toward HCC in vivo. Both histological and flow cytometric analysis showed that significantly fewer hMSCs localized within 97H-CCL15-shRNA xenografts compared with 97H-green fluorescent protein xenografts after intravenous delivery PMID: 26763650 Loss of SMAD4 was significantly associated with CCL15 expression. PMID: 26341919 follicular thyroid carcinoma (FTC) might induce tumor associated macrophages (TAMs) infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from follicular adenoma PMID: 26875556 two inflammatory cytokines, MIP-1delta and MIP-3alpha, are able to increase mesenchymal stromal cells migration in vitro PMID: 25579056 we investigated a role of CCL15 in upregulating ICAM-1 in endothelial cells PMID: 23690481 CCL15 is elevated in human asthma and correlates with disease severity . PMID: 22092970 Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551 increased secretion by macrophages during Mycobacterium tuberculosis infection PMID: 20016943 truncation of NH2-terminal amino acid residues increases agonistic potency on CC chemokine receptors 1 and 3 PMID: 11832479 Lkn-1 activates the ERK pathway by transducing the signal through G(i)/G(o) protein, PLC, PKC delta and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes PMID: 12759139 These observations demonstrate that the two NF-kappaB binding sites are essential for phorbol myristate acetate-induced leukotactin-1 (Lkn-1)/CCL15 expression in human monocytes. PMID: 15179048 CCL15(25-92) has in vitro and in vivo angiogenic activity PMID: 15251437 alanine-aspartic acid residues preceding the first cysteine at the NH(2)-terminus are essential for the binding and biological activity of leukotactin-1 PMID: 15894113 Compared with full-length CCL15, proteolytically processed CCL15 isoforms with N-terminal deletions display increased potency to induce calcium fluxes and chemotactic activity on monocytes and to induce adhesiveness of mononuclear cells to fibronectin. PMID: 16034099 Transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells. PMID: 16364464 Results point to an involvement of the CCL15-CCR1 axis in the pathophysiology of chronic renal failure. PMID: 16737685 factor NF-kappaB plays an important role in regulation of LZIP expression, and LZIP expression regulates the monocyte cell migration induced by Lkn-1 PMID: 17296613 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Tissue Specificity	Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
Database Links	HGNC: 10613 OMIM: 601393 KEGG: hsa:6359 STRING: 9606.ENSP00000432034 UniGene: Hs.272493

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Code	CSB-AP000581HU
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Recombinant Human C-C motif chemokine 15 protein(CCL15) (Active)

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