Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
Alternative Names
C-C motif chemokine 15; C-C motif chemokine ligand 15; CC chemokine 3; CCL15; CCL15(29-92); CCL15_HUMAN; Chemokine (C C motif) ligand 15; Chemokine CC-2; Chemokine CC2; HCC-2; HCC2; HMRP 2B; HMRP2B; Leukotactin 1; Leukotactin-1; LKN-1; LKN1; Macrophage inflammatory protein 5; MIP 1D; MIP-1 delta; MIP-1D; MIP-5; MIP1 delta; MIP1d; MIP5; MRP 2B; Mrp-2b; Mrp2b; NCC 3; NCC-3; NCC3; New CC chemokine 3; OTTHUMP00000163955; SCYA15; SCYL3; Small inducible cytokine A15; Small inducible cytokine subfamily A (Cys Cys); member 15; Small-inducible cytokine A15; SY15
Species
Homo sapiens (Human)
Expression Region
22-113aa
Complete Sequence
QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI
Protein Length
Full Length of Mature Protein
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human CCL15 protein is expressed in E. coli and covers the full length of the mature 22-113aa sequence. This tag-free protein is provided as a lyophilized powder, allowing for easy reconstitution with sterile water or buffer. With a purity of >97% as determined by SDS-PAGE and HPLC, our recombinant CCL15 also maintains a low endotoxin level of less than 1.0 EU/µg, as measured by the LAL method. The protein retains full biological activity, as demonstrated by its effectiveness in a chemotaxis bioassay using human T-lymphocytes, with an activity concentration range of 1.0-10 ng/ml.
C-C motif chemokine 15 (CCL15) is a member of the CC chemokine family and plays a crucial role in regulating immune cell trafficking and function. As such, understanding the functions and mechanisms of CCL15 is vital in uncovering the roles it plays in the immune system and the development of potential therapeutic interventions for immune-related diseases.
