Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

Instructions
Code CSB-E09173h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name matrix extracellular phosphoglycoprotein
Alternative Names Matrix extracellular phosphoglycoprotein ELISA Kit; MEPE ELISA Kit; MEPE_HUMAN ELISA Kit; OF45 ELISA Kit; Osteoblast/osteocyte factor 45 ELISA Kit
Abbreviation MEPE
Uniprot No. Q9NQ76
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MEPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 89  
Range % 84-93  
1:10 Average % 98  
Range % 95-102  
1:20 Average % 84  
Range % 80-88  
1:40 Average % 90  
Range % 85-94  
Recovery
The recovery of human MEPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-102  
EDTA plasma (n=4) 105 99-110  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.603 2.769 2.686 2.516  
500 1.967 2.075 2.021 1.851  
250 1.290 1.194 1.242 1.072  
125 0.631 0.679 0.655 0.485  
62.5 0.476 0.506 0.491 0.321  
31.2 0.341 0.357 0.349 0.179  
15.6 0.254 0.246 0.250 0.080  
0 0.170 0.169 0.170    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Promotes renal phosphate excretion and modulates mineralization.
Gene References into Functions
  1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage. PMID: 23570370
  2. Mice deficient in an ortholog of MEPE show increased bone mass and are resistant to aging-related bone loss. PMID: 12421822
  3. results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals. PMID: 22341070
  4. MEPE or the MEPE-derived acidic serine aspartate-rich MEPE-associated motif peptide may contribute to decreased bone mineralization in Oncogenic osteomalacia patients. PMID: 21739089
  5. Among migraineurs with aura rs7698623 in MEPE (OR = 6.37; 95% CI 3.15-12.90; p = 2.7x10(-7)) and rs4975709 in IRX4 (OR = 5.06; 95% CI 2.66-9.62; p = 7.7x10(-7)) appeared to be associated with ischemic stroke. PMID: 21779381
  6. Sclerostin acts through regulation of the PHEX/MEPE axis at the preosteocyte stage and serves as a master regulator of physiologic bone mineralization. PMID: 21312267
  7. MEPE may play a regulatory role in periodontal ligament cell osteogenic differentiation. PMID: 19031789
  8. These results suggest that abnormal MEPE cleavage occurs when PHEX activity is deficient in humans. PMID: 20581062
  9. This study demonstrates the importance of posttranslational modification for the site-specific activity of MEPE and its ASARM peptide. PMID: 19998030
  10. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
  11. MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21. Expressed during odontogenesis. PMID: 12489176
  12. There is evidence for a hormone/enzyme/extracellular matrix protein cascade involving fibroblastic growth factor 23 (FGF23), a phosphate-regulating gene with homologies to (PHEX) and (MEPE)--REVIEW PMID: 12791601
  13. MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone PMID: 15108058
  14. The results are consistent with MEPE being involved in phosphate and bone metabolism in a normal population. PMID: 15292364
  15. MEPE expression is higher when cells proliferate, whereas it is downregulated as cells differentiated PMID: 18547474
  16. MEPE is a candidate phosphatonin involved in phosphate homeostasis, acting in both the kidney and the gastrointestinal tract PMID: 19005008
  17. MEPE is expressed in salivary gland ducts. PMID: 15329369
  18. MEPE is expressed on the luminal surface of kidney proximal tubules. PMID: 15954904

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Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Expressed by osteoblasts. Secreted from oncogenic hypophosphataemic tumors.
Database Links

HGNC: 13361

OMIM: 605912

KEGG: hsa:56955

STRING: 9606.ENSP00000354341

UniGene: Hs.676357

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