Human neuromedin U (NMU) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

neuromedin U

Alternative Names

Neuromedin U 25 ELISA Kit; Neuromedin U ELISA Kit; Neuromedin-U-25 ELISA Kit; NmU 25 ELISA Kit; NMU ELISA Kit; NmU-25 ELISA Kit; NMU_HUMAN ELISA Kit

Abbreviation

NMU

Uniprot No.

P48645

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cerebrospinal fluid (CSF), tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human NMU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-98
1:2	Average %	92
	Range %	86-98
1:4	Average %	94
	Range %	84-98
1:8	Average %	94
	Range %	88-98

Recovery

The recovery of Human NMU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-102
EDTA plasma (n=4)	88	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.585	2.387	2.486	2.314
10	2.058	1.896	1.977	1.805
5	1.375	1.304	1.340	1.168
2.5	0.852	0.879	0.866	0.694
1.25	0.574	0.552	0.563	0.391
0.625	0.369	0.384	0.377	0.205
0.312	0.271	0.265	0.268	0.096
0	0.172	0.171	0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human NMU ELISA Kit was designed for the quantitative measurement of Human NMU protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Function
(From Uniprot)

Ligand for receptors NMUR1 and NMUR2. Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.; Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release although its potency is much lower than that of neuromedin precursor-related peptide 36.; Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release from lactotroph cells in the pituitary gland, probably via the hypothalamic dopaminergic system.

Gene References into Functions

Results identified NMU as highly expressed in non-small cell lung cancer (NSCLC) tissues and cell lines. Furthermore, integrative bioinformatics analyses demonstrate that NMU may confer the alectinib resistance in NSCLC via multiple mechanisms. PMID: 30096454
NMU might contribute to progression of NMUR2-positive breast cancer PMID: 28423716
The anti-tumorigenic effect of HAND2-AS1 in endometrioid endometrial carcinoma was mediated by down-regulating neuromedin U expression. PMID: 29107108
Results indicate a mechanism of action for neuromedin U (NmU) in HER2-overexpressing breast cancer that enhances resistance to HER2-targeted drugs through conferring cancer stem cell (CSC) characteristics and expansion of the CSC phenotype. PMID: 28340506
This study shows an association between a NMU haplotype and anthropometric indices, mainly linked to fat mass, which appears to be age- and sex-specific in children. PMID: 28235053
Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC. PMID: 27279246
polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
our results defined NmU as a candidate drug response biomarker for HER2-overexpressing cancers and as a candidate therapeutic target to limit metastatic progression and improve the efficacy of HER-targeted drugs. PMID: 24876102
a role for NMU gene through interaction with ADRB2 gene in bone strength regulation PMID: 23936460
Inactivation of the von Hippel-Lindau tumour suppressor gene induces Neuromedin U expression in renal cancer cells. PMID: 21791076
[review] Taken together with its vascular actions, NMU may be a functional link between energy balance and the cardiovascular system and may provide a future target for therapies directed against the disorders that comprise metabolic syndrome. PMID: 19519756
These results suggest that NMU plays a role in feeding behavior and catabolic functions via corticotropin-releasing hormone. PMID: 14623274
NmU expression is related to Myb and that the NmU/NMU1R axis constitutes a previously unknown growth-promoting autocrine loop in myeloid leukemia cells PMID: 15187020
irreversible binding of NmU to its receptors PMID: 15331768
Overexpression of neuromedin U is associated with bladder tumor formation, lung metastasis and cancer cachexia PMID: 16878152
Amino acid variants in NMU associate with overweight and obesity, suggesting that NMU is involved in energy regulation in humans. PMID: 16984985
NMU & its cancer-specific receptors, as well as its target genes, are frequently overexpressed in clinical samples of lung cancer and in cell lines, and that those gene products play indispensable roles in the growth and progression of lung cancer cells. PMID: 17018595
NmU may be involved in the HGF-c-Met paracrine loop regulating cell migration, invasiveness and dissemination of pancreatic ductal adenocarcinoma. PMID: 19118941

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Subcellular Location

Secreted.

Protein Families

NmU family

Tissue Specificity

Expressed throughout the enteric nervous system with highest levels being found in the jejunum.

Database Links

HGNC: 7859

OMIM: 605103

KEGG: hsa:10874

STRING: 9606.ENSP00000264218

UniGene: Hs.418367

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Code	CSB-EL015902HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.