Code | CSB-E09484h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human PON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 88 | ||||
Range % | 80-92 | |||||
1:200 | Average % | 98 | ||||
Range % | 91-105 | |||||
1:400 | Average % | 103 | ||||
Range % | 92-110 | |||||
1:800 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of human PON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-98 | ||||
EDTA plasma (n=4) | 95 | 90-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human paraoxonase (PON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PON1 in different samples, including serum, plasma, cell culture supernates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PON1 bound in the initial step.
PON1 is a phase-I enzyme involved in the hydrolysis of organophosphate esters. As a high-density lipoprotein (HDL)-associated esterase, PON1 can hydrolyze oxidized LDL-cholesterol, with potential atheroprotective effects. IT also mediates protection against lipid oxidation. PON1 not only has a weak paraoxonase activity but also exhibits anti-oxidant and anti-inflammatory activities. It is implicated in several human diseases, including diabetes mellitus and atherosclerosis. Low PON1 activity has been related to the elevated risk of major cardiovascular events.
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