Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cardiovascular
Alternative Names
A esterase 1; A-esterase 1; Aromatic esterase 1; Arylesterase 1; Arylesterase B type; ESA; Esterase A; K 45; K-45; MVCD5; Paraoxonase 1; Paraoxonase; Paraoxonase B type; Paraoxonase, plasma; Paraoxonase1; PON 1; PON; PON1; PON1_HUMAN; Serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; Serum paraoxonase/arylesterase 1
Species
Homo sapiens (Human)
Expression Region
2-355aa
Target Protein Sequence
AKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCEL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression of recombinant human PON1 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal GST tag sequence to the designated sequence encoding the 2-355aa of the human PON1 protein. This N-terminal 6xHis-tagged recombinant human PON1 protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant PON1 protein migrated to the band of about 55 kDa molecular weight.
Commonly, the expression of PON1 has been mainly found in liver. PON1, as the high-density lipoprotein (HDL)-associated serum enzyme, it exhibits a broad substrate specificity. Low PON1 activity found in children may increase their susceptibility to organophosphates. PON1 is responsible for protecting against exposure to some organophosphorus (OP) pesticides as well as vascular disease. Many findings suggested that PON1 participates in the activities of pesticide sensitivity, cardiovascular disease, and drug metabolism. Meanwhile, PON1 as the antioxidant enzyme, it has been recently confirmed as potential prognostic predictor of acute ischemic stroke. Certain studies indicated that Decreased PON1 activity and PON1 polymorphisms are associated with chronic kidney disease in type 2 diabetes mellitus. More important, researchers recently implied the association of PON1 polymorphisms activity and colorectal cancer predisposition.
