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| Code | CSB-E14339h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 83-93 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 80-103 | ||||
| EDTA plasma (n=4) | 96 | 86-106 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human KLKB1 ELISA Kit was designed for the quantitative measurement of Human KLKB1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.34 mU/mL.
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Does the ELISA detect both the zymogen and the cleaved Klkb1 or only the zymogen?
