Human plasma kallikrein(KLKB1) ELISA kit

Instructions
Code CSB-E14339h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name kallikrein B, plasma (Fletcher factor) 1
Alternative Names KLK3, PPK, Fletcher factor|kininogenin|plasma kallikrein B1|plasma kallikrein heavy chain|plasma kallikrein light chain
Abbreviation KLKB1
Uniprot No. P03952
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 9.38 mU/mL-600 mU/mL
Sensitivity 2.34 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %96
Range %89-100
1:200Average %90
Range %85-98
1:400Average %87
Range %83-93
1:800Average %92
Range %88-96
Recovery
The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9480-103
EDTA plasma (n=4)9686-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
6002.101 2.207 2.154 2.048
3001.695 1.687 1.691 1.585
1501.261 1.278 1.270 1.164
750.842 0.867 0.855 0.749
37.50.503 0.514 0.509 0.403
18.750.345 0.368 0.357 0.251
9.380.201 0.211 0.206 0.100
00.105 0.107 0.106  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Does the ELISA detect both the zymogen and the cleaved Klkb1 or only the zymogen?

A:
Thanks for your inquiry.
CSB-E14339h
This kit is designed against full length.
Pls refer to: https://www.uniprot.org/uniprot/P03952.Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease Prekallikrein deficiency (PKK deficiency)
Subcellular Location Secreted
Protein Families Peptidase S1 family, Plasma kallikrein subfamily
Database Links

HGNC: 6371

OMIM: 229000

KEGG: hsa:3818

STRING: 9606.ENSP00000264690

UniGene: Hs.237642

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