Human plasma kallikrein(KLKB1) ELISA kit

Code CSB-E14339h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human KLKB1 ELISA Kit was designed for the quantitative measurement of Human KLKB1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.34 mU/mL.

Target Name kallikrein B, plasma (Fletcher factor) 1
Alternative Names Fletcher factor ELISA Kit; kallikrein B plasma ELISA Kit; Kallikrein B plasma (Fletcher factor) 1 ELISA Kit; Kallikrein B; plasma 1 ELISA Kit; Kallikrein B1 ELISA Kit; Kininogenin ELISA Kit; KLK3 ELISA Kit; KLKB1 ELISA Kit; KLKB1_HUMAN ELISA Kit; PKK ELISA Kit; PKKD ELISA Kit; Plasma kallikrein ELISA Kit; Plasma kallikrein B1 ELISA Kit; Plasma kallikrein light chain ELISA Kit; Plasma prekallikrein ELISA Kit; PPK ELISA Kit; Prekallikrein ELISA Kit
Abbreviation KLKB1
Uniprot No. P03952
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 9.38 mU/mL-600 mU/mL
Sensitivity 2.34 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %96
Range %89-100
1:200Average %90
Range %85-98
1:400Average %87
Range %83-93
1:800Average %92
Range %88-96
Recovery
The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9480-103
EDTA plasma (n=4)9686-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
6002.101 2.207 2.154 2.048
3001.695 1.687 1.691 1.585
1501.261 1.278 1.270 1.164
750.842 0.867 0.855 0.749
37.50.503 0.514 0.509 0.403
18.750.345 0.368 0.357 0.251
9.380.201 0.211 0.206 0.100
00.105 0.107 0.106  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Does the ELISA detect both the zymogen and the cleaved Klkb1 or only the zymogen?

A:
Thanks for your inquiry.
CSB-E14339h
This kit is designed against full length.
Pls refer to: https://www.uniprot.org/uniprot/P03952.Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Gene References into Functions
  1. We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development. PMID: 29186361
  2. Plasma kallikrein (PLK)-dependent transforming growth factor-beta (TGF-beta) activation could be detected in isolated pancreatic stellate cells (PSCs) from chronic pancreatitis (CP) and pancreatic cancer. PMID: 28187111
  3. High molecular weight kinin functions as a cofactor for PK activation in the presence of nucleic acids in a manner consistent with classic models of contact activation. PMID: 28124063
  4. genetic polymorphism is associated with C1 Inhibitor deficiency angioedema age of onset in European families PMID: 29130992
  5. these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1 PMID: 26566265
  6. von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth. PMID: 27581227
  7. KLKB1 mRNA expression is a putative molecular biomarker in chronic lymphocytic leukemia. PMID: 25891023
  8. Genotyping these subjects revealed that the carriers of the minor alleles at the two loci- F12 and KLKB1 had a significant association with reduced levels of active plasma renin. PMID: 26969407
  9. 2 genetic loci (kininogen 1 and kallikrein B) influencing key components of the RAAS, consistent with the close interrelation between the kallikrein-kinin system and the RAAS. PMID: 25477429
  10. PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
  11. Prekallikrein deficiency in a family is due to a new mutation (Arg541Gln) in exon 14. PMID: 25075649
  12. FXI may have a role in risk of ischemic stroke, but not myocardial infarct; FXII and prekallikrein may not have a role in either PMID: 24977287
  13. The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II. PMID: 23672780
  14. Investigate the relationship between circulating PK levels in children with metabolic syndrome and CVD risk factors because PK may be involved in the progression of the disease state. PMID: 20725119
  15. Case Report: Fresh frozen plasma transfusion before coronary artery bypass corrects prekallikrein deficiency. PMID: 20135073
  16. plasma kallikrein and FXIa activate pro-HGF in vitro PMID: 12372819
  17. the effect of kininogen degradation by human neutrophil elastase (HNE) on kinin generation by tissue and plasma kallikreins. PMID: 12887060
  18. prekallikrein preferential binding to endothelial cells contributes to their anticoagulant nature PMID: 12944405
  19. Crystallization and x-ray crystal structure determination have yielded the first three-dimensional views of the catalytic domain of plasma kallikrein PMID: 16199530
  20. Our findings suggested that common genetic variation in the KLKB1 gene might contribute to the risk of hypertension in the northern Han Chinese population. PMID: 17318641
  21. study demonstrates by immunohistochemistry that plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues PMID: 17696780
  22. three-dimensional structural data for prekallikrein (PK); interaction between PK and high-molecular-weight kininogen is mediated by two discrete surfaces formed by the PK A1, A2 and A4 domains with charge likely to be a critical component of the binding PMID: 17922805
  23. Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway PMID: 18160576
  24. Kallikrein is responsible for the cleavage of factor H. PMID: 18413232
  25. prekallikrein is an enzyme that can cleave high-molecular-weight kininogen to release bradykinin, and this reaction is inhibited by C1 inhibitor. PMID: 19342086
  26. NPY3-35 is a new peptide generated by plasma kallikrein PMID: 19620246

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Involvement in disease Prekallikrein deficiency (PKK deficiency)
Subcellular Location Secreted.
Protein Families Peptidase S1 family, Plasma kallikrein subfamily
Database Links

HGNC: 6371

OMIM: 229000

KEGG: hsa:3818

STRING: 9606.ENSP00000264690

UniGene: Hs.237642

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