Product Details

Uniprot No.

Target Names

KLKB1

Alternative Names

Plasma kallikrein (EC 3.4.21.34) (Fletcher factor) (Kininogenin) (Plasma prekallikrein) (PKK) [Cleaved into: Plasma kallikrein heavy chain, Plasma kallikrein light chain], KLKB1, KLK3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human KLKB1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The KLKB1 recombinant monoclonal antibody is designed for the detection of human KLKB1 protein in ELISA and IHC applications. This antibody is produced using recombinant DNA technology, whereby the gene coding for the KLKB1 monoclonal antibody is synthesized after sequencing the cDNA of the KLKB1 antibody-producing hybridomas. The hybridomas are generated by fusing B cells isolated from the animal that was immunized with a human KLKB1-derived peptide with myeloma cells. The synthesized gene is then inserted into a vector and transfected into cells for cultivation. Finally, the KLKB1 recombinant monoclonal antibody is purified through affinity chromatography from the cell culture supernatant.

The KLKB1 protein, also known as plasma kallikrein, is an enzyme that plays a role in the blood coagulation system and the kinin-kallikrein system. In the blood coagulation system, KLKB1 cleaves high-molecular-weight kininogen (HK) to produce bradykinin, a vasoactive peptide that regulates blood pressure, smooth muscle contraction, and inflammation. In the kinin-kallikrein system, KLKB1 activates the plasma prekallikrein to kallikrein, which can then cleave HK to produce bradykinin. KLKB1 also activates coagulation factor XII (Hageman factor), which plays a role in the initiation of the intrinsic pathway of blood coagulation. Additionally, KLKB1 has been implicated in the regulation of angiogenesis and tumor growth.

Related Products

Target Background

Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Gene References into Functions

We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development. PMID: 29186361
Plasma kallikrein (PLK)-dependent transforming growth factor-beta (TGF-beta) activation could be detected in isolated pancreatic stellate cells (PSCs) from chronic pancreatitis (CP) and pancreatic cancer. PMID: 28187111
High molecular weight kinin functions as a cofactor for PK activation in the presence of nucleic acids in a manner consistent with classic models of contact activation. PMID: 28124063
genetic polymorphism is associated with C1 Inhibitor deficiency angioedema age of onset in European families PMID: 29130992
these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1 PMID: 26566265
von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth. PMID: 27581227
KLKB1 mRNA expression is a putative molecular biomarker in chronic lymphocytic leukemia. PMID: 25891023
Genotyping these subjects revealed that the carriers of the minor alleles at the two loci- F12 and KLKB1 had a significant association with reduced levels of active plasma renin. PMID: 26969407
2 genetic loci (kininogen 1 and kallikrein B) influencing key components of the RAAS, consistent with the close interrelation between the kallikrein-kinin system and the RAAS. PMID: 25477429
PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
Prekallikrein deficiency in a family is due to a new mutation (Arg541Gln) in exon 14. PMID: 25075649
FXI may have a role in risk of ischemic stroke, but not myocardial infarct; FXII and prekallikrein may not have a role in either PMID: 24977287
The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II. PMID: 23672780
Investigate the relationship between circulating PK levels in children with metabolic syndrome and CVD risk factors because PK may be involved in the progression of the disease state. PMID: 20725119
Case Report: Fresh frozen plasma transfusion before coronary artery bypass corrects prekallikrein deficiency. PMID: 20135073
plasma kallikrein and FXIa activate pro-HGF in vitro PMID: 12372819
the effect of kininogen degradation by human neutrophil elastase (HNE) on kinin generation by tissue and plasma kallikreins. PMID: 12887060
prekallikrein preferential binding to endothelial cells contributes to their anticoagulant nature PMID: 12944405
Crystallization and x-ray crystal structure determination have yielded the first three-dimensional views of the catalytic domain of plasma kallikrein PMID: 16199530
Our findings suggested that common genetic variation in the KLKB1 gene might contribute to the risk of hypertension in the northern Han Chinese population. PMID: 17318641
study demonstrates by immunohistochemistry that plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues PMID: 17696780
three-dimensional structural data for prekallikrein (PK); interaction between PK and high-molecular-weight kininogen is mediated by two discrete surfaces formed by the PK A1, A2 and A4 domains with charge likely to be a critical component of the binding PMID: 17922805
Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway PMID: 18160576
Kallikrein is responsible for the cleavage of factor H. PMID: 18413232
prekallikrein is an enzyme that can cleave high-molecular-weight kininogen to release bradykinin, and this reaction is inhibited by C1 inhibitor. PMID: 19342086
NPY3-35 is a new peptide generated by plasma kallikrein PMID: 19620246

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Involvement in disease

Prekallikrein deficiency (PKK deficiency)

Subcellular Location

Secreted.

Protein Families

Peptidase S1 family, Plasma kallikrein subfamily

Database Links

HGNC: 6371

OMIM: 229000

KEGG: hsa:3818

STRING: 9606.ENSP00000264690

UniGene: Hs.237642

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