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| Code | CSB-E13721h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:10 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| The recovery of human SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-95 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human SFRP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SFRP4 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SFRP4 levels in the sample and is measured at 450 nm through a microplate reader.
SFRP4 is expressed in the intestinal tract, endometrium, pancreas, stomach, liver, heart, and mammary gland. Ample studies have shown that SFRP4 regulates apoptosis and differentiation of tumor cells and is an important antagonist of the Wnt pathway. Loss of SFRP4 expression has been observed in most tumors, including ovarian cancer, malignant pituitary tumors, endometrial cancer, and acute myeloid leukemia. However, upregulation of SFRP4 is found in chemotherapy-sensitive ovarian cancer and breast cancer. Additionally, SFRP4 is implicated in many diseases including obesity, type 2 diabetes (T2D), and cancer.
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