Product Details

Uniprot No.

Q6FHJ7

Target Names

SFRP4

Alternative Names

Secreted frizzled-related protein 4 (sFRP-4) (Frizzled protein, human endometrium) (FrpHE), SFRP4, FRPHE

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human SFRP4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14C1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SFRP4 (Secreted frizzled-related protein 4) functions as a soluble modulator of Wnt signaling, a pathway fundamental to embryonic development, tissue homeostasis, and disease progression. By acting as a decoy receptor that sequesters Wnt ligands, SFRP4 influences processes ranging from adipogenesis and bone metabolism to tumor suppression. Its dysregulation has been implicated in metabolic disorders, cardiovascular disease, and various cancers, making it a compelling target for researchers investigating these interconnected biological systems.

This recombinant monoclonal antibody, generated in rabbit and derived from clone 14C1, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in longitudinal studies.

Validation data demonstrates robust performance across multiple experimental platforms. In western blot applications, the antibody detects SFRP4 in human cell lines including HeLa, A549, and U-251MG, as well as in mouse uterus and rat uterus and heart tissues, confirming cross-species reactivity. The observed band at approximately 60 kDa, rather than the predicted 40 kDa molecular weight, likely reflects post-translational glycosylation characteristic of secreted proteins. Immunofluorescence studies in A549 cells reveal clear cytoplasmic localization with minimal background, while flow cytometry analysis shows distinct positive population shifts compared to isotype controls.

With validated performance in ELISA, western blot, immunofluorescence, and flow cytometry, this antibody provides flexibility for researchers studying Wnt pathway modulation, metabolic signaling, or tumor microenvironment dynamics across human, mouse, and rat experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.

Gene References into Functions

Data suggest that factors derived from conditioned medium of adipose-derived mesenchymal stem cells and sFRP4 significantly decrease cell viability and cell migration of breast tumor cells; this is accompanied by enhanced apoptosis via inhibition of canonical Wnt signal transduction. PMID: 29277675
increased SFRP4 and ficolin-3 levels are significantly associated with gestational diabetes mellitus development and might be important risk factors for this pregnancy complication. PMID: 29491225
High SFRP4 expression is associated with mesothelioma. PMID: 28534940
Epicardial adipose tissue and circulating SFRP4 expression levels were increased with patients with coronary artery disease. PMID: 29037197
Serum SFRP-4 levels were significantly higher in the type 2 diabetes group compared to the impaired glucose tolerance and normal glucose tolerance groups. PMID: 29455547
Studied role of Secreted Frizzled-Related Protein 4 (sFRP4) in angiostasis thru nitric oxide-cGMP cell signaling. PMID: 28267592
Progesterone regulated SFRP4 gene expression plays a role in uterine leiomyoma. PMID: 28637297
SFRP4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
SFRP4 is significantly increased in patients with different types of diabetes. PMID: 26882051
Weak sFRP4 expression appeared to predict aggressive behavior, and was associated with recurrence/progression of GH-secreting pituitary adenomas. PMID: 26935389
A decreased risk of lung cancer was found for the genotype combination of DKK3 and sFRP4. PMID: 26457595
Down-regulation of SFRP4 is associated with recurrence in Endometrioid Adenocarcinoma. PMID: 26397159
proliferation of IGFBP5-mutated cancer cells is selectively blocked by IGF-1R inhibitors PMID: 26868304
concentrations of serum SFRP4 in T2DM and impaired glucose tolerance subjects were increased and were correlated closely with glycose metabolic disorder, the first-phase of glucose-stimulated insulin secretion and chronic low-grade inflammation PMID: 26041556
sFRP4 expression is inversely related to the aggressiveness of pituitary adenomas, and acts as a tumor suppressor. PMID: 24917361
Wnt3a can modulate intracellular localisation and secretion of sFRP4. PMID: 25805505
We found that miR-203 was significantly downregulated in OSF tissues compared to that in normal buccal mucosa tissues, and that miR-203 negatively regulated secreted SFRP4 and positively regulated TM4SF1 PMID: 25872484
Serum WISP2 correlated directly with fatty acid binding protein 4. Serum SFRP5 did not differ between obese (n=32) vs. nonobese (n=25) PCOS women, but reference women had lower SFRP5 (p<5x10(-6) as compared to both PCOS groups). PMID: 25089371
Abdominal adipose tissue is a major contributor for circulating-SFRP4; SFRP4 has an important role in obese adipose tissue pathophysiology. PMID: 25322919
SFRP4 concentrations are associated with impaired glucose and triglyceride metabolism PMID: 25408147
findings suggest that an epigenetic event is critically involved in the pathogenesis of psoriasis, and the downregulation of SFRP4 by CpG island methylation is one possible mechanism contributing to the hyperplasia of epidermis in the disease PMID: 25825452
Increased SFRP4 expression may not always correlate with an increase in apoptosis, possibly due to the complex interactions between different signalling pathways. [Review] PMID: 25501511
total of three potential inhibitors of SFRP4 were found, namely cyclothiazide, clopamide and perindopril. These inhibitors showed significant interactions with SFRP4 as compared to other inhibitors as well as control (acetohexamide). PMID: 25019556
Our results suggest that the sFRP3 c.970C>G and sFRP4 c.958C>A polymorphisms may be genetic factors associated with the prevalence of osteoporosis at the femoral neck in postmenopausal Korean women. PMID: 24662300
High SFRP4 gene methylation is associated with ovarian cancer infected with high risk human papillomavirus. PMID: 24761891
ZAR1 and SFRP4 qMSP could be used as potential biomarker for CC diagnosis PMID: 24787227
Decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe preeclampsia. PMID: 23322712
This raises the possibility that SFRP4 may be used both diagnostically and therapeutically in epithelial ovarian cancer. PMID: 23326605
SFRP4 caused reduced glucose tolerance through decreased islet expression of Ca(2+) channels and suppressed insulin exocytosis. PMID: 23140642
haplotype genotypes of secreted frizzled-related protein 4 (sFRP4) c.958C>A and c.1019G>A polymorphisms are genetic factors that affect changes in bone mineral density of the femoral neck after hormone therapy in postmenopausal Korean women PMID: 22668815
sFRP4's cysteine-rich domain was seen to inhibit tube formation of endothelial cells. In addition, the netrin-like domain promoted endothelial cell death and may also inhibit angiogenesis. PMID: 23035359
genetic variation in sFRP4 has an influence on hip fracture risk, percentage body fat and height in a Danish male population. However, we were unable to replicate these results in an independent Belgian population. PMID: 22608881
These results support a role for SFRP4 as a tumor suppressor gene in ovarian cancers. PMID: 22363760
Data show that decidualization was associated with increased expression of 428 genes including SCARA5 (181-fold), DKK1 (71-fold) and PROK1 (32-fold), and decreased expression of 230 genes including MMP-7 (35-fold) and SFRP4 (21-fold). PMID: 21858178
results indicate that the coding regions of INHBA, SFRP4 and HOXA10 do not harbour mutations responsible for linkage to endometriosis in the families studied PMID: 21576276
sFRP4 is expressed in human GCs and its expression declines during late antral follicular growth PMID: 21307090
Methylation and unmethylation of SFRP4 were both detected in CA46, HL60 and U937 cell lines. PMID: 20030932
the secreted frizzled-related protein (sFRP4) c1019G>A polymorphism may be one of the genetic factors affecting lumbar spine Bone minera density in postmenopausal Korean women. Risk AA genotype was 6.39 times higher than GG PMID: 20613673
SFRP4 promoter methylation is aberrant in mesothelioma. PMID: 20596629
sFRP-4 displays phosphatonin-like properties. PMID: 12952927
lack of secreted frizzled-related protein 4 leads to progression of prostate cancer PMID: 14760084
Methylation silencing of SFRP4 may play an important role in aberrant Wnt activation in mesothelioma even in the absence of beta-catenin PMID: 15705870
sFRP-4 increases renal P(i) excretion by reducing Na(+)-P(i)-IIa transporter abundance in the brush border of the proximal tubule through enhanced internalization of the protein. PMID: 16151791
In contrast to other sFRP family members, sFRP-4 expression appears to be upregulated in colorectal carcinoma PMID: 16356838
The SFRP4 protein gene plays an important role in the pathogenesis of bladder tumor and can be detected using cellular DNA extracted from urine samples. PMID: 16609023
sFRP4 is an inhibitor of prostate cancer growth and invasion in vitro independent of androgen receptor (AR) signaling. PMID: 17476687
These data suggest sFRP4 promotes epidermal differentiation. PMID: 18938133
sFRP4 may be a potential biomarker for monitoring the progression of ADPKD. PMID: 18945944
Dkk1 and sFRP4 perform an important function in adipogenesis in human adipose tissue-derived mesenchymal stem cells. PMID: 19040566
sFRP4 has a role in placental development and implantation, and may be an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis and a band of apoptosis in the underlying decidua deep into the trophoblast. PMID: 19146776

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Involvement in disease

Pyle disease (PYL)

Subcellular Location

Secreted. Note=Cytoplasmic in ovarian tumor cells.

Protein Families

Secreted frizzled-related protein (sFRP) family

Tissue Specificity

Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, exp

Database Links

HGNC: 10778

OMIM: 265900

KEGG: hsa:6424

STRING: 9606.ENSP00000410715

UniGene: Hs.658169

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Code	CSB-RA104640A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), A549 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), Mouse uterus tissue lysate(30µg), Rat uterus tissue lysate(30µg), Rat heart tissue lysate(30µg) All lanes: SFRP 4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 40 kDa Observed band size: 60 kDa Exposure time：2min Immunofluorescence staining of A549 cell with CSB-RA104640A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A549 cells stained with CSB-RA104640A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SFRP4 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background