Human stromal cell-derived factor 1 alpha (SDF1A) ELISA kit

Instructions
Code CSB-EQ027490HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human stromal cell-derived factor 1 alpha (SDF1A) ELISA kit allows for the in vitro quantitative determination of SDF1A concentrations in multiple biological fluids, including serum, plasma, cell culture supernates, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for immunology research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which SDF1A in the samples or standards are sandwiched between pre-coated SDF1A antibody and Biotin-conjugated SDF1A antibody. HRP-avidin is then added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1A bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of SDF1A in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

SDF1A is the most abundant SDF1 isoform in the brain and is expressed by stromal cells. It is retained in the marrow by heparans and proteoglycans. The SDF1A/CXCR4 interaction is a migrational stimulus and leads to the retention of progenitors. Interfering with interactions of SDF1A and CXCR4 blocks the in vivo homing of hemopoietic stem cells (HPSCs) to the bone marrow (BM) and mobilizes stem cells for release into the blood. SDF1A ligation has also been involved in the arrest of progenitor cell cycling, which facilitates a transition into quiescence.

Target Name stromal cell-derived factor 1 alpha (SDF1A)
Alternative Names 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; AI174028 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) ELISA Kit; Chemokine (C-X-C motif) ligand 12 ELISA Kit; Chemokine CXC motif ligand 12 ELISA Kit; cxcl12 ELISA Kit; hIRH ELISA Kit; hSDF-1 ELISA Kit; Intercrine reduced in hepatomas ELISA Kit; IRH ELISA Kit; OTTHUMP00000019491 ELISA Kit; PBSF ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; SCYB12 ELISA Kit; SDF 1 ELISA Kit; SDF-1 ELISA Kit; SDF-1-alpha(3-67) ELISA Kit; SDF-1a ELISA Kit; SDF-1b ELISA Kit; SDF1_HUMAN ELISA Kit; SDF1A ELISA Kit; SDF1B ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; Stromal cell-derived factor 1 delta ELISA Kit; Stromal cell-derived factor 1 gamma ELISA Kit; Stromal cell-derived factor 1a ELISA Kit; Stromal cell-derived factor-1 alpha ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; Tlsf ELISA Kit; TLSF-a ELISA Kit; TLSF-b ELISA Kit; Tlsfa ELISA Kit; Tlsfb ELISA Kit; TPAR1 ELISA Kit
Abbreviation SDF1A/CXCL12
Uniprot No. P48061
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SDF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %88
Range %85-92
1:10Average %96
Range %91-102
1:20Average %92
Range %86-98
1:40Average %90
Range %85-96
Recovery
The recovery of human SDF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-96
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.750 2.732 2.741 2.640
10002.144 2.136 2.140 2.039
5001.163 1.144 1.154 1.053
2500.630 0.628 0.629 0.528
1250.348 0.324 0.336 0.235
62.50.260 0.246 0.253 0.152
31.250.210 0.201 0.206 0.105
00.105 0.097 0.101  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SDF1A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled SDF1A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Gene References into Functions
  1. CXCL12-positive cases exhibited shorter disease-free survival rates compared to CXCL12-negative cases. PMID: 30182340
  2. this study shows an essential role of CXCR7, together with CXCR4, in the control of normal and malignant hematopoietic cell migration and homing induced by CXCL12. PMID: 29433559
  3. CXCL12 rs1801157 is independently associated with Human papillomavirus infection and exerts influence in high grade intraepithelial lesions development PMID: 30227860
  4. silencing of CXCL12 had a protective effect on podocyte injury, which may be through inhibiting CXCL12/STAT3 signaling pathway. PMID: 29508174
  5. The CXCL12/SDF1 protein expression is a good prognostic biomarker in breast cancer. PMID: 29800557
  6. the CXCL12-CXCR4 axis promotes the migration, invasion, and EMT processes in B-CPAP cells, at least partly, by activating the NF-kappaB signaling pathway. PMID: 29316404
  7. Study shows that CXCL12 methylation-mediated epigenetic regulation of gene expression in papillary thyroid carcinoma (PTC). The study was the first to perform an reduced representation bisulfite sequencing analysis for PTC and suggested that CXCL12 may contribute to PTC development by methylation-mediated epigenetic regulation of gene expression. PMID: 28272462
  8. results demonstrate that non-oxidizable HMGB1 induce a sustained cardiac fibroblasts migration despite the redox state of the environment and by altering CXCL12/CXCR4 axis. This affects proper cardiac remodeling after an infarction. PMID: 28716707
  9. A basis for understanding how multiple elements in the sequence encoding the 3'UTR of the CXCL12 gene regulates its transcription and insights about diseases involving abnormal CXCL12alpha expression. PMID: 30266500
  10. High SDF-1 expression is associated with bladder cancer progression. PMID: 30015971
  11. High CXCL12 expression is associated with metastasis in colon cancer. PMID: 29305742
  12. MiR-125b functions as an important downstream mediator upon the activation of CXCL12/CXCR4 axis. PMID: 28176874
  13. CXCL12-related rs18011517 polymorphism was more frequent in non-Hodgkin lymphoma patients: it might be associated with non-Hodgkin lymphoma pathogenesis and outcome PMID: 30197351
  14. Data suggest that CXCL12 and its receptor CXCR4 are critical in maintaining homeostasis, specifically during hematopoiesis. Present clinical trials (especially in hematological tumors) are testing whether adding CXCR4 inhibitors to impair tumor dissemination will increase effectiveness of ongoing anti-cancer treatments. (CXCL12 = C-X-C motif chemokine ligand 12; CXCR4 = C-X-C motif chemokine receptor-4) [REVIEW] PMID: 29288743
  15. BCP-ALL cells actively migrate toward mesenchymal stromal cells (MSCs) in a CXCL12-dependent manner. PMID: 28619846
  16. Serum CXCR4 and CXCL12 levels increase significantly in septic neonates and they are valuable marker in diagnosis of neonatal sepsis. Serum concentrations of both chemokines represent promising novel biomarkers for neonatal sepsis. PMID: 28562124
  17. CXCL12 and CXCR4 polymorphisms may be risk factors for hepatocellular carcinoma (HCC), and they may be potential HCC markers. PMID: 29741398
  18. Stromal cell derived factor-1/C-X-C chemokine receptor type 4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3beta/beta-catenin pathways. PMID: 28067275
  19. EGFR Over-expression and Mutations Leading to Biological Characteristics Changes of Human Lung Adenocarcinoma Cells through CXCR4/CXCL12 Signaling Pathway PMID: 30037369
  20. Serum CXCL12, but not CXCR4, Is Associated with Head and Neck Squamous Cell Carcinomas. PMID: 29693336
  21. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)alpha and the stromal derived factor (SDF)1/CXC chemokine receptor type 4 (CXCR4) axis are abnormally expressed in Intrauterine adhesions endometrium. PMID: 29568895
  22. HIV-1 infectors with SDF-1 3'A polymorphism have a higher chance of developing late AIDS. PMID: 30053458
  23. the SDF1/CXCR4 signaling pathway is involved in Lowintensity pulsed ultrasoundpromoted periodontal ligament stem cell migration. PMID: 29620151
  24. These results suggest that SDF1 (e.g. presented on proteoglycans) can rapidly activate integrins in an allosteric manner by binding to site 2 in the absence of CXCR4. The allosteric integrin activation by SDF1 is a novel target for drug discovery PMID: 29301984
  25. CXCL12 single nucleotide polymorphisms association with the risk of hypertension in Chinese Han population. PMID: 30180964
  26. These results suggest a key role for the CXCR4-CXCL12 chemokine axis in breast cancer progression and highlight the prognostic importance of this chemokine axis for breast cancer survival. PMID: 29516917
  27. serum SDF-1 is increased in and may be a potential useful marker for primary biliary cholangitis PMID: 29414663
  28. disruption of the CXCR4/CXCL12 axis by CXCR4 antagonist AMD3100 blocked the contribution of both cancer and stromal cells to the metastatic cascade in the liver. PMID: 29436696
  29. SDF-1 alpha overexpression in bone marrow-derived stromal stem cells promotes bone generation as indicated by osteogenesis and angiogenesis. PMID: 29758548
  30. These results suggest that SDF1, as an inflammatory cytokine, induces MMP expression in human endplate chondrocytes and that ECM remodeling in the degenerated cartilage endplate may be a favorable factor of endogenous stem cell homing into the nucleus pulposus for regeneration in vivo PMID: 29207021
  31. data demonstrate that: (i) hypoxia does not affect the capacity of EPCs to support the angiogenic process; (ii) the absence of either VEGF-A or SDF-1 from EPC-CM can be rescued by the presence of the other one, so that the overall angiogenic effects remain unchanged; and (iii) and the concomitant deletion of VEGF-A and SDF-1 from EPC-CM impairs its pro-angiogenic effect, both in vitro and in vivo. PMID: 27943613
  32. estrogen may promote the progression of ER-negative breast cancer by stimulating cancer-associated fibroblasts to secrete SDF-1alpha, which can recruit MDSCs to the tumor microenvironment to exert tumor-promoting effects PMID: 27996037
  33. Data support the importance of SDF-1 and CXCR4 expression for loco-regional control and overall survival in HNSCC after primary radiochemotherapy. PMID: 29061496
  34. Data (including data from studies in knockout mice) suggest that adipocyte autocrine function involving SDF1 regulates insulin resistance; SDF1 gene expression correlates with insulin-desensitized conditions in adipocytes but not other tissues (liver, skeletal muscle); adipocyte-specific ablation of Sdf1 enhances insulin sensitivity in adipose tissues and in whole body. PMID: 29581126
  35. Study reports that stromal cell-derived factor-1alpha elevated or therapeutically administered in ischemic wounded tissue can stimulate both local endothelial cells (EC) and bone marrow-derived endothelial progenitor cells (EPC) to express reciprocally E-selectin/ligand pairs and thereby enhance EPC-EC interactions. PMID: 27713493
  36. Authors produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect in murine adult NSCs migration and survival in vitro. We showed CXCL12(5-67) does not promote NSCs migration, but does induce cell death. PMID: 28623786
  37. a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. PMID: 28536953
  38. Expression upregulation of mir31 was also validated using GEO data sets. PMID: 27597234
  39. Differential expression of SDF-1 receptor CXCR4 in molecularly defined forms of inherited thrombocytopenias. PMID: 28032520
  40. A role for CXCl12 in bladder cancer [review] PMID: 29022185
  41. Intravenous administration of rhSDF-1alpha accelerates reendothelialization in the aneurysm neck after flow diverter implantation. PMID: 28159982
  42. These findings suggest a possibility that cells at the sites of MELF pattern had acquired increased invasiveness through the function of the CXCL14-CXCR4 and CXCL12-CXCR4 axes. PMID: 28277316
  43. study suggested that the SDF-1 rs1801157 polymorphism may serve as a risk factor for cancer development among Asians, especially an increased risk of urologic and lung cancers PMID: 27265091
  44. no significant association was found between SDF1 polymorphism and HIV susceptibility; a protective effect of SDF1 on AIDS progression and death was seen; in conclusion, SDF1 polymorphism exerts a moderate protective effect against AIDS disease deterioration in some specific populations PMID: 29420545
  45. Findings indicate that induction of EMT, increased migration and survival depend, in MCF-7 and H460 cells, on the release of FHC control on two pathways, namely the iron/ROS metabolism and CXCR4/CXCL12 axis. PMID: 28774348
  46. serum level is higher in preeclamptic women PMID: 28001450
  47. a defect of CXCL12 promoter histone acetylation may represent an additional process participating in CXCL12 expression extinction in colon cancer PMID: 28418886
  48. The findings indicated that SDF-1alpha/CXCR4 signaling pathway might be associated with the clinicopathological features and prognosis of patients with nasopharyngeal carcinoma. PMID: 28559386
  49. CXCL12-CXCR7 axis accelerates migration and invasion of pancreatic cancer cells through mTOR and Rho/ROCK pathways, and predicts poor prognosis of pancreatic cancer PMID: 27542220
  50. the role of CXCL12 in multiple sclerosis, with an emphasis on CXCL12 serum concentrations and its gene polymorphism at position +801 (Review) PMID: 27894110

Show More

Hide All

Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and i
Database Links

HGNC: 10672

OMIM: 600835

KEGG: hsa:6387

STRING: 9606.ENSP00000379140

UniGene: Hs.522891

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1