Recombinant Human Stromal cell-derived factor 1 protein(CXCL12) (Active)

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Code CSB-AP000741HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
Target Names CXCL12
Uniprot No. P48061
Research Area Immunology
Alternative Names 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C-X-C motif chemokine 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Chemokine (C-X-C motif) ligand 12; Chemokine CXC motif ligand 12; cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; OTTHUMP00000019491 ; PBSF; Pre-B cell growth-stimulating factor; SCYB12; SDF 1; SDF-1; SDF-1-alpha(3-67); SDF-1a; SDF-1b; SDF1_HUMAN; SDF1A; SDF1B; Stromal cell-derived factor 1; Stromal cell-derived factor 1 delta ; Stromal cell-derived factor 1 gamma ; Stromal cell-derived factor 1a ; Stromal cell-derived factor-1 alpha ; Thymic lymphoma cell-stimulating factor; Tlsf; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1
Species Homo sapiens (Human)
Source E.Coli
Expression Region 22-89aa
Mol. Weight 8.0 kDa
Protein Length Full Length of Mature Protein of Isoform Alpha
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Gene References into Functions
  1. CXCL12-positive cases exhibited shorter disease-free survival rates compared to CXCL12-negative cases. PMID: 30182340
  2. this study shows an essential role of CXCR7, together with CXCR4, in the control of normal and malignant hematopoietic cell migration and homing induced by CXCL12. PMID: 29433559
  3. CXCL12 rs1801157 is independently associated with Human papillomavirus infection and exerts influence in high grade intraepithelial lesions development PMID: 30227860
  4. silencing of CXCL12 had a protective effect on podocyte injury, which may be through inhibiting CXCL12/STAT3 signaling pathway. PMID: 29508174
  5. The CXCL12/SDF1 protein expression is a good prognostic biomarker in breast cancer. PMID: 29800557
  6. the CXCL12-CXCR4 axis promotes the migration, invasion, and EMT processes in B-CPAP cells, at least partly, by activating the NF-kappaB signaling pathway. PMID: 29316404
  7. Study shows that CXCL12 methylation-mediated epigenetic regulation of gene expression in papillary thyroid carcinoma (PTC). The study was the first to perform an reduced representation bisulfite sequencing analysis for PTC and suggested that CXCL12 may contribute to PTC development by methylation-mediated epigenetic regulation of gene expression. PMID: 28272462
  8. results demonstrate that non-oxidizable HMGB1 induce a sustained cardiac fibroblasts migration despite the redox state of the environment and by altering CXCL12/CXCR4 axis. This affects proper cardiac remodeling after an infarction. PMID: 28716707
  9. A basis for understanding how multiple elements in the sequence encoding the 3'UTR of the CXCL12 gene regulates its transcription and insights about diseases involving abnormal CXCL12alpha expression. PMID: 30266500
  10. High SDF-1 expression is associated with bladder cancer progression. PMID: 30015971
  11. High CXCL12 expression is associated with metastasis in colon cancer. PMID: 29305742
  12. MiR-125b functions as an important downstream mediator upon the activation of CXCL12/CXCR4 axis. PMID: 28176874
  13. CXCL12-related rs18011517 polymorphism was more frequent in non-Hodgkin lymphoma patients: it might be associated with non-Hodgkin lymphoma pathogenesis and outcome PMID: 30197351
  14. Data suggest that CXCL12 and its receptor CXCR4 are critical in maintaining homeostasis, specifically during hematopoiesis. Present clinical trials (especially in hematological tumors) are testing whether adding CXCR4 inhibitors to impair tumor dissemination will increase effectiveness of ongoing anti-cancer treatments. (CXCL12 = C-X-C motif chemokine ligand 12; CXCR4 = C-X-C motif chemokine receptor-4) [REVIEW] PMID: 29288743
  15. BCP-ALL cells actively migrate toward mesenchymal stromal cells (MSCs) in a CXCL12-dependent manner. PMID: 28619846
  16. Serum CXCR4 and CXCL12 levels increase significantly in septic neonates and they are valuable marker in diagnosis of neonatal sepsis. Serum concentrations of both chemokines represent promising novel biomarkers for neonatal sepsis. PMID: 28562124
  17. CXCL12 and CXCR4 polymorphisms may be risk factors for hepatocellular carcinoma (HCC), and they may be potential HCC markers. PMID: 29741398
  18. Stromal cell derived factor-1/C-X-C chemokine receptor type 4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3beta/beta-catenin pathways. PMID: 28067275
  19. EGFR Over-expression and Mutations Leading to Biological Characteristics Changes of Human Lung Adenocarcinoma Cells through CXCR4/CXCL12 Signaling Pathway PMID: 30037369
  20. Serum CXCL12, but not CXCR4, Is Associated with Head and Neck Squamous Cell Carcinomas. PMID: 29693336
  21. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)alpha and the stromal derived factor (SDF)1/CXC chemokine receptor type 4 (CXCR4) axis are abnormally expressed in Intrauterine adhesions endometrium. PMID: 29568895
  22. HIV-1 infectors with SDF-1 3'A polymorphism have a higher chance of developing late AIDS. PMID: 30053458
  23. the SDF1/CXCR4 signaling pathway is involved in Lowintensity pulsed ultrasoundpromoted periodontal ligament stem cell migration. PMID: 29620151
  24. These results suggest that SDF1 (e.g. presented on proteoglycans) can rapidly activate integrins in an allosteric manner by binding to site 2 in the absence of CXCR4. The allosteric integrin activation by SDF1 is a novel target for drug discovery PMID: 29301984
  25. CXCL12 single nucleotide polymorphisms association with the risk of hypertension in Chinese Han population. PMID: 30180964
  26. These results suggest a key role for the CXCR4-CXCL12 chemokine axis in breast cancer progression and highlight the prognostic importance of this chemokine axis for breast cancer survival. PMID: 29516917
  27. serum SDF-1 is increased in and may be a potential useful marker for primary biliary cholangitis PMID: 29414663
  28. disruption of the CXCR4/CXCL12 axis by CXCR4 antagonist AMD3100 blocked the contribution of both cancer and stromal cells to the metastatic cascade in the liver. PMID: 29436696
  29. SDF-1 alpha overexpression in bone marrow-derived stromal stem cells promotes bone generation as indicated by osteogenesis and angiogenesis. PMID: 29758548
  30. These results suggest that SDF1, as an inflammatory cytokine, induces MMP expression in human endplate chondrocytes and that ECM remodeling in the degenerated cartilage endplate may be a favorable factor of endogenous stem cell homing into the nucleus pulposus for regeneration in vivo PMID: 29207021
  31. data demonstrate that: (i) hypoxia does not affect the capacity of EPCs to support the angiogenic process; (ii) the absence of either VEGF-A or SDF-1 from EPC-CM can be rescued by the presence of the other one, so that the overall angiogenic effects remain unchanged; and (iii) and the concomitant deletion of VEGF-A and SDF-1 from EPC-CM impairs its pro-angiogenic effect, both in vitro and in vivo. PMID: 27943613
  32. estrogen may promote the progression of ER-negative breast cancer by stimulating cancer-associated fibroblasts to secrete SDF-1alpha, which can recruit MDSCs to the tumor microenvironment to exert tumor-promoting effects PMID: 27996037
  33. Data support the importance of SDF-1 and CXCR4 expression for loco-regional control and overall survival in HNSCC after primary radiochemotherapy. PMID: 29061496
  34. Data (including data from studies in knockout mice) suggest that adipocyte autocrine function involving SDF1 regulates insulin resistance; SDF1 gene expression correlates with insulin-desensitized conditions in adipocytes but not other tissues (liver, skeletal muscle); adipocyte-specific ablation of Sdf1 enhances insulin sensitivity in adipose tissues and in whole body. PMID: 29581126
  35. Study reports that stromal cell-derived factor-1alpha elevated or therapeutically administered in ischemic wounded tissue can stimulate both local endothelial cells (EC) and bone marrow-derived endothelial progenitor cells (EPC) to express reciprocally E-selectin/ligand pairs and thereby enhance EPC-EC interactions. PMID: 27713493
  36. Authors produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect in murine adult NSCs migration and survival in vitro. We showed CXCL12(5-67) does not promote NSCs migration, but does induce cell death. PMID: 28623786
  37. a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. PMID: 28536953
  38. Expression upregulation of mir31 was also validated using GEO data sets. PMID: 27597234
  39. Differential expression of SDF-1 receptor CXCR4 in molecularly defined forms of inherited thrombocytopenias. PMID: 28032520
  40. A role for CXCl12 in bladder cancer [review] PMID: 29022185
  41. Intravenous administration of rhSDF-1alpha accelerates reendothelialization in the aneurysm neck after flow diverter implantation. PMID: 28159982
  42. These findings suggest a possibility that cells at the sites of MELF pattern had acquired increased invasiveness through the function of the CXCL14-CXCR4 and CXCL12-CXCR4 axes. PMID: 28277316
  43. study suggested that the SDF-1 rs1801157 polymorphism may serve as a risk factor for cancer development among Asians, especially an increased risk of urologic and lung cancers PMID: 27265091
  44. no significant association was found between SDF1 polymorphism and HIV susceptibility; a protective effect of SDF1 on AIDS progression and death was seen; in conclusion, SDF1 polymorphism exerts a moderate protective effect against AIDS disease deterioration in some specific populations PMID: 29420545
  45. Findings indicate that induction of EMT, increased migration and survival depend, in MCF-7 and H460 cells, on the release of FHC control on two pathways, namely the iron/ROS metabolism and CXCR4/CXCL12 axis. PMID: 28774348
  46. serum level is higher in preeclamptic women PMID: 28001450
  47. a defect of CXCL12 promoter histone acetylation may represent an additional process participating in CXCL12 expression extinction in colon cancer PMID: 28418886
  48. The findings indicated that SDF-1alpha/CXCR4 signaling pathway might be associated with the clinicopathological features and prognosis of patients with nasopharyngeal carcinoma. PMID: 28559386
  49. CXCL12-CXCR7 axis accelerates migration and invasion of pancreatic cancer cells through mTOR and Rho/ROCK pathways, and predicts poor prognosis of pancreatic cancer PMID: 27542220
  50. the role of CXCL12 in multiple sclerosis, with an emphasis on CXCL12 serum concentrations and its gene polymorphism at position +801 (Review) PMID: 27894110
  51. nitration on Tyr7 under inflammatory conditions is a novel natural posttranslational regulatory mechanism of CXCL12 which may downregulate the CXCR4-mediated inflammatory and tumor-promoting activities of CXCL12 PMID: 27566567
  52. this study shows that stem cell autocrine CXCL12/CXCR4 stimulates invasion and metastasis of esophageal cancer PMID: 28193907
  53. The CXCR7/CXCL12 axis is involved in lymph node and liver metastasis of gastric cancer. PMID: 28533662
  54. SDF-1 level was significantly elevated in the subchondral bone of human osteoarthritis samples. In the cell studies, the results showed SDF-1 plays an important role in osteogenic differentiation of mesenchymal stem cells. PMID: 28131784
  55. Serum CXCL12 concentrations are enhanced after ICH and CXCL12 in serum has the potential to reflect severity and prognosis following hemorrhagic stroke PMID: 28526531
  56. Activation of CXCL12/CXCR4 axis in vascular endothelial cells stimulates the angiogenesis. PMID: 29381400
  57. miR-221-3p upregulation prevents IL-1beta-induced extracellular matrix (ECM) degradation in chondrocytes. Protection of ECM degradation by miR-223-3p occurs via SDF1/CXCR4 signaling. miR-221-3p is identified as a novel potential therapeutic target for osteoarthritis. PMID: 28236026
  58. circulating plasma levels of SDF-1 are associated with increasing age and independently associated with lower total hip bone mineral density in both men and wome PMID: 28246930
  59. BH3 mimetics might prove therapeutically useful in the treatment of malignant peripheral nerve sheath tumor cells by virtue of their ability to suppress CXCL12 expression. PMID: 28055968
  60. Antibodies against SDF1 and CXCR4 blocked the positive effect of DUX4 overexpression on bone marrow-derived mesenchymal stem cells migration PMID: 27556182
  61. Circulating CXCL12 serum levels at admission is a useful biomarker to predict functional outcome and mortality following Ischemic Stroke. PMID: 26780459
  62. SDF-1 upregulates the number of adherent tumor cells by responding to matrix stiffness via promoting the expression of integrin beta1, which is abolished by blocking of integrin beta1. These results may provide a novel point of view for the mechanism of "organ specificity" phenomenon in tumor metastasis, which in turn contribute to a rational development of new drugs for cancer PMID: 28478797
  63. This study explores the role of the CXCL12/CXCR4 signaling pathway in primary tumor radiation response in cervical cancer and the effect on lymph node metastases. Cervical cancer xenografts derived directly from patients were treated with targeted, fractionated RT and weekly cisplatin, with or without the CXCR4 inhibitor Plerixafor PMID: 27697997
  64. The combination of FTY720 with the SPHK1 inhibitor SKI-II results in synergistic inhibition of MM growth. CXCR4/CXCL12-enhanced expression correlates with reduced MM cell sensitivity to both FTY720 and SKI-II inhibitors, and with SPHK1 coexpression in both cell lines and primary MM bone marrow (BM) samples, suggesting regulative cross-talk between the CXCR4/CXCL12 and SPHK1 pathways in MM cells PMID: 27697999
  65. This study demonstrates that in neural progenitor cell-derived glioblastoma cells under hypoxic conditions, CXCL12/CXCR4 signaling elicits an autocrine-positive feedback mechanism, which promotes survival and cell-cycle progression. Our study brings new mechanistic insights which warrant the use of drugs blocking CXCL12 as adjuvant agents to target hypoxia-induced glioblastoma progression, prevent resistance to treatment PMID: 27542769
  66. we identified a novel function of CXCL12 in promoting the 'Warburg' effect in acute myeloid leukemia cells PMID: 26952837
  67. the association of the SDF1-3'A polymorphism with HIV-1 infection only in women, but not to CD4+ T-lymphocyte categories, viral load levels in patients with HIV-1/AIDS PMID: 26859597
  68. ADAM17 is a Western diet-inducible enzyme activated by CXCL12-CXCR4 signaling, suggesting the pathway: Western diet-->CXCL12-->CXCR4-->ADAM17-->TGFalpha-->EGFR. ADAM17 might serve as a druggable target in chemoprevention strategies PMID: 27489286
  69. Concomitant aberrant chemokine CXCL12 (CXCL12) methylation and high programmed death-ligand 1 (PD-L1) expression was significantly associated with shorter biochemical recurrence (BCR)-free survival. PMID: 27462860
  70. Results show that CXCL12 is highly expressed in metastatic lymph node (MNL) of non-small cell lung neoplasm (NSCLC) and is associated with poor prognosis which suggest that CXCL12 may play a role in tumor progression of NSCLC. PMID: 29032612
  71. The CXCL12 rs1801157 G > A polymorphism may affect CLL development, disease progression as well as response to treatment. PMID: 27173875
  72. KIT exon 11 codons 557-558 deletion enhanced CXCL12-mediated GIST cell migration. PMID: 26936919
  73. the relationship between SDF-1/CXCR4 axis and leukemia cells is explored PMID: 27282562
  74. Curcumin up-regulates Slit-2 and down-regulates the expression of CXCR4, SDF-1, MMP2 and MMP9 in Ishikawa, Hec- 1B and primary human endometrial carcinoma cells. PMID: 28402926
  75. CXCL12 is involved in the pathogenesis and healing of H. pylori-induced peptic ulcer. PMID: 27269177
  76. Together, these data indicate that different mechanisms govern the flow response across GSCs, but that within a single patient, there are subpopulations of GSCs that respond to flow via either CD44- or CXCR4-CXCL12 mechanisms. PMID: 27775742
  77. Data indicate that a constitutively monomeric CXCL12 variant reproduced the G protein-dependent and beta-arrestin-dependent responses that are associated with normal CXCR4 signaling and lead to cell migration. PMID: 28325822
  78. HS had no effects on the binding of CXCL12alpha to CXCR4 or its biological activity, suggesting that this polysaccharide controls CXCL12 in an isoform-specific manner. PMID: 27803285
  79. The level of CXCR4 protein expression was significantly higher in all cellular compartments of the endometriotic lesions compared to control endometrium. CXCL12 protein expression was also higher in endometriotic lesions and was greatest in the epithelial compartment. CXCL12 was increased more in the condition media of cultured endometriosis than in controls as measured by ELISA. PMID: 27729562
  80. CCR1 activation potently induces multiple myeloma PC migration toward CCL3 while abrogating the multiple myeloma PC migratory response to CXCL12. PMID: 28855206
  81. High CXCL12 expression is associated with gemcitabine resistance in pancreatic cancer. PMID: 27175473
  82. Myocardial ischemia induces SDF-1alpha release in cardiac surgery patients. PMID: 27055858
  83. CXCL12 expression may be a useful marker for predicting the outcome in patients with esophageal squamous cell carcinoma and is a potentially new therapeutic target PMID: 27439769
  84. our findings indicate the functional role of HBx in regulating the stem-like properties of OV6(+) CSCs in HCC through the MDM2/CXCL12/CXCR4/beta-catenin signaling axis, and identify HBx, MDM2, CXCR4 and OV6 as a novel prognostic pathway and potential therapeutic targets for patients with HBV-related HCC patients PMID: 28102846
  85. study revealed that the CXCL12-CXCR4 interaction is crucial for stromal cell contact-mediated early B lymphoid and pDC differentiation from immature hematopoietic and early T lymphoid precursors with a multilineage differentiation potential but not for stromal cell contact-independent generation of early T lymphoid precursors. PMID: 28842468
  86. SDF-1, CXCR4 ligand, was highly expressed in mesenchymal stem cells when co-cultured with degenerated nucleus pulposus cells. Inhibition of SDF-1 using CXCR4 antagonist AMD3100 abolished the MSCs-induced decrease in the mechanical moduli and increased biological activity of degenerated NPCs, suggesting a crucial role for SDF-1/CXCR4 signaling. PMID: 27163878
  87. Cathepsin K cleavage of SDF-1alpha inhibits its chemotactic activity towards glioblastoma stem-like cells. PMID: 28040478
  88. High CXCL12 expression is associated with glioblastoma resistance to radiotherapy. PMID: 27370398
  89. SDF-1 promoted the proliferation of gastric cancer SGC-7901 cells, the phosphorylation of ERK1/2, p38 and CXCR7 knockdown distinctly reversed these changes; the proliferation stimulated with SDF-1 was attenuated by U0126 (MEK1/2 inhibitor). PMID: 28281844
  90. Results show that SDF-1 and IL-6 expression level is regulated by CHEK2 in breast stromal fibroblasts. PMID: 27484185
  91. This study shows that expression of SDF-1alpha, eNOS and VEGF were significantly higher in uterine leiomyoma than myometrium with a different expression pattern according to the size of uterine leiomyoma. However, expressions of visfatin and leptin had no significant differences between the two groups. PMID: 28010141
  92. Data demonstrated that sustained expression of CXCL12 by MSCs in the primary tumour site inhibits metastasis through reduction of CXCR7, while, in the presence of TGFbeta, this CXCL12 effect of MSCs on tumour cells is relieved. PMID: 27669436
  93. The study demonstrated that monomeric CXCL12 was the fundamental form, which played important roles in endothelial progenitor cells' proliferation, migration, and tube-formation. PMID: 28487111
  94. the A allele and AA genotype of the SDF1- rs1111875 polymorphism produce a significant risk of MI. the GG genotype of the SDF1-rs1111875 polymorphism provides a protective effect on MI in a recessive model (GG vs. AA+AG) PMID: 28650670
  95. These results suggest that that CXCL14 is a positive allosteric modulator of CXCR4 via CXCL12 that enhances the potency of CXCR4 ligands. PMID: 28360196
  96. High SDF1 expression is associated with Hepatocellular Carcinoma. PMID: 27593937
  97. GATA2 deficiency is associated with impaired membrane expression and chemotactic dysfunctions of CXCR4. PMID: 26710799
  98. this study shows that CXCL12 conveys epithelial-mesenchymal transition -promoting signals in neuroendocrine tumors cells through CXCR4 PMID: 28186979
  99. tumor microenvironment stimulation down-regulated the migration of CCR7-expressing tumor cells toward CCL21 and inhibited the formation of directional protrusions toward CCL21 in a novel 3-dimensional hydrogel system. PMID: 26936935
  100. These findings suggest the tumor suppressor functions of CXCL12 in cervical cancer. PMID: 26970955

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and i
Database Links

HGNC: 10672

OMIM: 600835

KEGG: hsa:6387

STRING: 9606.ENSP00000379140

UniGene: Hs.522891

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