Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

Code CSB-EL025550HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q8WVF2
Lead Time 7-14 working days
Abbreviation UCMA
Target Name upper zone of growth plate and cartilage matrix associated
Alias C10orf49, unique cartilage matrix-associated protein
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human UCMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %82-91
1:2Average %95
Range %91-99
1:4Average %96
Range %93-99
1:8Average %87
Range %82-93
Recovery
The recovery of human UCMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8481-89
EDTA plasma (n=4)8380-86
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.578 2.596 2.587 2.394
52.168 2.115 2.142 1.949
2.51.536 1.512 1.524 1.331
1.250.969 0.958 0.964 0.771
0.6250.598 0.572 0.585 0.392
0.3120.455 0.456 0.456 0.263
0.1560.371 0.398 0.385 0.192
00.195 0.190 0.193
References
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Function May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families UCMA family
Tissue Specificity Predominantly expressed in resting chondrocytes.
Database Links

HGNC: 25205

KEGG: hsa:221044

STRING: 9606.ENSP00000367952

UniGene: Hs.112998

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