Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

Instructions
Code CSB-EL025550HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name upper zone of growth plate and cartilage matrix associated
Alternative Names UCMA ELISA Kit; C10orf49 ELISA Kit; Unique cartilage matrix-associated protein [Cleaved into: Unique cartilage matrix-associated protein C-terminal fragment ELISA Kit; Ucma-C ELISA Kit; Gla-rich protein ELISA Kit; GRP)] ELISA Kit
Abbreviation UCMA
Uniprot No. Q8WVF2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human UCMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %82-91
1:2Average %95
Range %91-99
1:4Average %96
Range %93-99
1:8Average %87
Range %82-93
Recovery
The recovery of human UCMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8481-89
EDTA plasma (n=4)8380-86
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.578 2.596 2.587 2.394
52.168 2.115 2.142 1.949
2.51.536 1.512 1.524 1.331
1.250.969 0.958 0.964 0.771
0.6250.598 0.572 0.585 0.392
0.3120.455 0.456 0.456 0.263
0.1560.371 0.398 0.385 0.192
00.195 0.190 0.193
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

Target Data

Function May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.
Gene References into Functions
  1. GRP might act as a novel molecular mediator linking inflammation and calcification events PMID: 28542410
  2. The study demonstrates the involvement of GRP in the two major molecular processes affecting osteoarthritis: mineralization and inflammation of articular tissue. PMID: 26337479
  3. Data suggest that novel splice variants of GRP are expressed in cartilage and other tissues (i.e. GRP-F5, GRP-F6); GRP-F1 is predominant splice variant expressed in adult tissues particularly in cartilage of patients with osteoarthritis. PMID: 24867294
  4. full exon resequencing of DNA samples from 17 chronic kidney disease patients (stage 5) and 121 healthy controls in a Swsedish population; the 138Thr > Ser polymorphism seems to be the only non-synonymous SNP found in the UCMA gene in a Swedish population PMID: 24409676
  5. Gla-rich protein (GRP) is a new vitamin K-dependent protein that can bind calcium through Gla residues. PMID: 18836183
  6. The present data strongly suggest an important function of Ucma in the early phase of chondrocyte differentiation. PMID: 17707622
  7. Ucma may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells PMID: 18156182

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families UCMA family
Tissue Specificity Predominantly expressed in resting chondrocytes.
Database Links

HGNC: 25205

KEGG: hsa:221044

STRING: 9606.ENSP00000367952

UniGene: Hs.112998

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