Human vascular endothelial cell growth factor E,VEGF-E ELISA Kit

Code CSB-E15022h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
platelet derived growth factor C
Alternative Names
Fallotein ELISA Kit; hSCDGF ELISA Kit; PDGF-C ELISA Kit; PDGFC ELISA Kit; PDGFC latent form ELISA Kit; PDGFC receptor-binding form ELISA Kit; PDGFC_HUMAN ELISA Kit; Platelet derived growth factor C ELISA Kit; Platelet-derived growth factor C; receptor-binding form ELISA Kit; SCDGF ELISA Kit; Secretory growth factor like protein ELISA Kit; Secretory growth factor like protein fallotein ELISA Kit; Spinal cord derived growth factor ELISA Kit; Spinal cord-derived growth factor ELISA Kit; VEGF E ELISA Kit; VEGF-E ELISA Kit
Abbreviation
PDGFC
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, urine, cell culture supernates, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %96
Range %90-100
1:4Average %98
Range %91-105
1:8Average %90
Range %82-98
Recovery
The recovery of human VEGF-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9183-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.806 2.712 2.759 2.659
2002.012 2.066 2.039 1.939
1001.473 1.384 1.429 1.329
500.958 0.893 0.926 0.826
250.494 0.473 0.484 0.384
12.50.321 0.301 0.311 0.211
6.250.254 0.249 0.252 0.152
00.102 0.097 0.100  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PDGFC ELISA Kit was designed for the quantitative measurement of Human PDGFC protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.
Gene References into Functions
  1. There are four platelet-derived growth factor (PDGF) genes (PDGFA, PDGFB, PDGFC and PDGFD) that reside on chromosomes 7, 22, 4 and 11. PMID: 28267575
  2. We conclude that PDGF-CC-induced blood-spinal cord barrier dysfunction can contribute to timing of amyotrophic lateral sclerosis onset PMID: 26687981
  3. High glucose-mediated induction of PDGF-C via ChREBP in mesangial cells contributes to the development of glomerular mesangial expansion in diabetes. PMID: 27033449
  4. PDGF-C up-regulation was mediated by the human embryonic lethal abnormal vision-like protein HuR, which stabilizes the PDGF-C transcript by binding to two predicted AU-rich elements (AREs) in the 3'-untranslated region (3'-UTR). PMID: 25383675
  5. Concomitant upregulation of PDGF-C with VEGF in Glioblastoma tumor cells. PMID: 24612214
  6. The results indicate that PDGF-C upregulation and calpain-3 downregulation are involved in the aggressiveness of malignant melanoma and suggest that modulators of these proteins PMID: 24126726
  7. Platelet-derived growth factor-C (PDGF-C) induces anti-apoptotic effects on macrophages through Akt and Bad phosphorylation. PMID: 24421315
  8. Data indicate uPA (plau) and PAI1 (Serpine1) were up-regulated in human PDGF-C Tg mice, suggesting that uPA could be compensating for the loss of tissue-type plasminogen activator (tPA) activity in PDGF-C Tg; tpa KO mice. PMID: 24269585
  9. High PDGF-C expression induces progressive fibrosis, chronic inflammation, neoangiogenesis and sinusoidal congestion resulting in hepatocellular carcinoma. PMID: 23929039
  10. PDGF-C is both angiogenic and a neuronal survival factor, and it is an important component of neurovascular crosstalk. [Review] PMID: 23714575
  11. Results indicate that the alpha-SMA inducing effect of M2 macrophages is in part mediated by secretion of PGDF-CC. PMID: 23182716
  12. Single nucleotide polymorphisms in the PDGF-C gene are linked to the development of oral clefts. PMID: 23029525
  13. Platelet-derived growth factor-C (PDGF-C) activation by serine proteases. PMID: 22035541
  14. Melanoma cell-derived platelet-derived growth factor (PDGF)-AA and PDGF-CC were identified as major mediators that signal through PDGFR-alpha and thus induce HAS2-mediated HA synthesis in fibroblasts PMID: 21993555
  15. Data suggest that the variant rs1425486 genotype could differentially affect targeting by miR-425 in ovarian cancer cells leading to higher PDGFC expression. PMID: 21118967
  16. PDGFC mRNA expression were down-regulated in biopsies containing infiltrated lymphocytes or thyroiditis. PMID: 19968886
  17. Upregulation of PDGFC expression may contribute to the clonal expansion of fibroid-derived smooth muscle cells in the development of uterine fibroids. PMID: 19553600
  18. Data suggest that five novel genes, LUM, PDE3B, PDGF-C, NRG1 and PKD2, have great potential for predicting the efficacy of cisplatin-based chemotherapy against OSCC. PMID: 19569180
  19. Dominant negative PDGF-C inhibits growth of Ewing family tumor cell lines. PMID: 12032822
  20. determination of PDGFC expression levels in medulloblastoma tumor cell lines PMID: 12176024
  21. PDGF-C has a higher structural homology to the vascular endothelial growth factor than to PDGF-B PMID: 12598536
  22. PDGF-C is constitutively expressed in the human kidney and is upregulated in podocytes and interstitial cells after injury/activation of these cells. PMID: 12707385
  23. In adult human tissues, PDGF-C was largely in smooth muscle. It binds & activate alpha PDGFR, & activates beta PDGFR when it is co-expressed with alpha PDGFR through heterodimer formation. PDGF C is likely to play a role in platelet biology. PMID: 15061151
  24. Plays a role in tissue remodeling. PMID: 15389578
  25. PDGF-C transgenic mice represent a unique model for the study of hepatic fibrosis progressing to tumorigenesis PMID: 15728360
  26. determine the molecular mechanism of tPA-mediated activation of PDGF-C dimer PMID: 15911618
  27. Expression of PDGF-C in leiomyomata was significantly higher (approximately 2.4-fold) than in the adjacent normal myometrium. PMID: 17482170
  28. PDGF-C and PDGF-D, like PDGF-A and PDGF-B, play important roles in atherosclerosis by stimulating MMP activity and influencing monocyte migration PMID: 18573494
  29. A novel splice variant of human PDGF-C (PDGF-Cb), which encodes an N-terminally truncated protein, lacking the signal peptide and CUB domain, is reported. PMID: 18588873
  30. The PDGF-C regulatory region SNP rs28999109 decreases promoter transcriptional activity and is associated with cleft lip with or without cleft palate (CL/P) PMID: 19092777
  31. some tumors may overcome inhibition of VEGF-mediated angiogenesis through upregulation of PDGF-C PMID: 19111878
  32. PDGFC was identified as target of EWS/ETS transcriptionalderegulation specific to EWS/FLI-1 chimeras in the Ewing Family Tumors suggesting that it may be a significant mediator of EWS/FLI driven oncogenesis. PMID: 11313995

Show More

Hide All

Subcellular Location
Cytoplasm, cytosol. Secreted. Nucleus. Cytoplasmic granule. Cell membrane.
Protein Families
PDGF/VEGF growth factor family
Tissue Specificity
Expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Expressed in medulloblastoma. In the kidney, constitutive
Database Links

HGNC: 8801

OMIM: 608452

KEGG: hsa:56034

STRING: 9606.ENSP00000422464

UniGene: Hs.570855

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X