Code | CSB-E09824h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human BACE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 90 | ||||
Range % | 82-97 | |||||
1:200 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:400 | Average % | 92 | ||||
Range % | 88-105 | |||||
1:800 | Average % | 96 | ||||
Range % | 85-101 |
The recovery of human BACE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 91 | 80-100 | ||||
EDTA plasma (n=4) | 98 | 92-105 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The ELISA Kit is designed to measure human BACE1 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of BACE1 in samples. The color intensity is measured at 450 nm via a microplate reader.
BACE1 is an aspartic protease that catalyzes the rate-limiting, initial cleavage at the β site of amyloid precursor protein (APP) and is followed by sequential intra-membrane processing at multiple sites by γ-secretase in the production of amyloid-β (Aβ). It is widely expressed in the brain, particularly in neurons, oligodendrocytes, and astrocytes, with particular abundance in various neuronal cell types. Inhibition of BACE1 proteolytic activity has been demonstrated to reduce Aβ generation and amyloid deposition, and thus specific inhibition of BACE1 by small molecules is a current focus for Alzheimer's disease therapy. In addition to the amyloidogenic pathway, BACE1 is involved in synaptic plasticity and synaptic homeostasis.
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