Code | CSB-RA177574A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The BACE1 recombinant monoclonal antibody can be effectively used for detecting human, mouse, and rat BACE1 proteins in both ELISA and WB applications. It is produced by employing recombinant DNA technology, where the BACE1 monoclonal antibody gene is synthesized after sequencing the cDNA of BACE1 antibody-producing hybridomas. These hybridomas are formed by fusing myeloma cells with B cells obtained from animals immunized with a synthetic peptide derived from human BACE1. Once the gene is synthesized, it is cloned into a vector. The recombinant vector is transfected into cells for cultivation. The resulting BACE1 recombinant monoclonal antibody is further purified via affinity chromatography from the cell culture supernatant.
The BACE1 protein is an enzyme that is primarily expressed in the brain and plays a key role in the production of beta-amyloid peptide, which is a major component of the amyloid plaques found in the brains of Alzheimer's disease patients. BACE1 cleaves the amyloid precursor protein (APP) at a specific site, resulting in the production of beta-amyloid. The accumulation of beta-amyloid is believed to be a major factor in the development and progression of Alzheimer's disease.
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