The Mouse Albumin (Alb) ELISA Kit is specifically designed to standardize the detection of mouse Alb in multiple biological solutions, including serum, plasma, and tissue homogenates. This kit employs the direct Competitive-ELISA mechanism to quantitatively measure the concentration of Alb. The Anti-mouse Alb antibody has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells with biotin-conjugated Alb. A competitive inhibition reaction is launched between Alb (standards or samples) and biotin-conjugated Alb with the pre-coated Alb antibody. After washing, HRP-avidin is added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells. HRP-TMB interaction elicits a chromogenic reaction. The color development is terminated, and solution color changes from blue to yellow. The color intensity is in opposition to the amount of Alb bound in the initial step. This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, precision less than 10%, and consistency between batches. See more details on the product instructions.
Albumin is the major serum protein that exerts multiple important physiological functions, including maintenance of colloidal osmotic pressure, provision of the bulk of plasma antioxidant activity, and binding of a wide variety of compounds. Albumin decreases for less than half the risk for post-paracentesis cardiocirculatory dysfunction and mortality related to spontaneous bacterial infection and triplicates the response to terlipressin in patients with hepatorenal syndrome, so it is an important tool in the management of patients with cirrhosis.