Mouse Albumin (Alb)ELISA Kit

Instructions
Code CSB-E13878m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Mouse Albumin (Alb) ELISA Kit is specifically designed to standardize the detection of mouse Alb in multiple biological solutions, including serum, plasma, and tissue homogenates. This kit employs the direct Competitive-ELISA mechanism to quantitatively measure the concentration of Alb. The Anti-mouse Alb antibody has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells with biotin-conjugated Alb. A competitive inhibition reaction is launched between Alb (standards or samples) and biotin-conjugated Alb with the pre-coated Alb antibody. After washing, HRP-avidin is added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells. HRP-TMB interaction elicits a chromogenic reaction. The color development is terminated, and solution color changes from blue to yellow. The color intensity is in opposition to the amount of Alb bound in the initial step. This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, precision less than 10%, and consistency between batches. See more details on the product instructions.

Albumin is the major serum protein that exerts multiple important physiological functions, including maintenance of colloidal osmotic pressure, provision of the bulk of plasma antioxidant activity, and binding of a wide variety of compounds. Albumin decreases for less than half the risk for post-paracentesis cardiocirculatory dysfunction and mortality related to spontaneous bacterial infection and triplicates the response to terlipressin in patients with hepatorenal syndrome, so it is an important tool in the management of patients with cirrhosis.

Target Name albumin
Alternative Names Alb 1 ELISA Kit; alb ELISA Kit; ALBU_MOUSE ELISA Kit; Albumin 1 ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
Abbreviation ALB
Uniprot No. P07724
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.781 μg/mL-200 μg/mL
Sensitivity 0.110 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess. 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of mouse albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with the anti-mouse Alb antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled Alb (100 x concentrate) (60 μl/bottle)
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (20 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute the HRP-avidin solution.
  • Two vial Sample Diluent (20 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Gene References into Functions
  1. findings suggest that HMGB1 induces the transcytosis of albumin via RAGE-dependent Src phosphorylation and Cav-1 phosphorylation. These studies revealed a new mechanism of HMGB1-induced endothelial hyperpermeability. PMID: 27572515
  2. Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase of kidney injury and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. These markers could detect higher risk of progression to CKD. PMID: 27028054
  3. Extending serum half-life of albumin by engineering neonatal Fc receptor (FcRn) binding. PMID: 24652290
  4. We provide evidence of a transcytosis within the kidney tubular system that protects albumin and IgG from lysosomal degradation, allowing these proteins to be recycled intact. PMID: 23970123
  5. albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation. PMID: 20529675
  6. No binding of albumin was observed at physiological pH to neonatal Fc receptor. At acidic pH, a 100-fold difference in binding affinity was observed. PMID: 20018855
  7. Cellular oxidant stress and advanced glycation endproducts of albumin: caveats of the dichlorofluorescein assay PMID: 11913966
  8. albumin enhancer/promoter was succesfully used for trasient transfection of fluorescent gene reporters (for cell sorting) in ES spontaneously differentiated into hepatocytes. PMID: 18942772

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Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

KEGG: mmu:11657

STRING: 10090.ENSMUSP00000031314

UniGene: Mm.16773

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