Mouse Allograft inflammatory factor 1(AIF1) ELISA kit

Instructions
Code CSB-EL001490MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name allograft inflammatory factor 1
Alternative Names Aif1 ELISA kit; Iba1Allograft inflammatory factor 1 ELISA kit; AIF-1 ELISA kit; Ionized calcium-binding adapter molecule 1 ELISA kit
Abbreviation AIF1
Uniprot No. O70200
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %86-98
1:2Average %99
Range %93-105
1:4Average %97
Range %90-104
1:8Average %95
Range %86-105
Recovery
The recovery of mouse AIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9390-98
EDTA plasma (n=4)9591-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.750 2.713 2.732 2.547
7502.145 2.101 2.123 1.938
3751.515 1.481 1.498 1.313
187.51.031 1.022 1.027 0.842
940.668 0.656 0.662 0.477
470.409 0.399 0.404 0.219
23.50.298 0.286 0.292 0.107
00.187 0.183 0.185  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Gene References into Functions
  1. Aif1 is important for bacterial and particle transcytosis in M cells. PMID: 28224999
  2. These findings indicate that the expression of AIF-1 in macrophages is critical for the activation of renal fibroblasts to a profibrotic phenotype. PMID: 29749461
  3. Macrophage-derived AIF-1 up-regulated reactive oxygen species production, adipokine TNFalpha, IL6, resistin release, and inhibited adiponectin secretion. PMID: 29788012
  4. A new subset of resident macrophages in the intestinal muscularis externa was discovered, identified as iba1(pos) CD169(neg). PMID: 27860408
  5. These results suggest that AIF-1 plays an important role in the mechanism underlying lung fibrosis, and may provide an attractive new therapeutic target. PMID: 29225172
  6. AIF-1 plays an important role in the mechanism underlying acute lung injury. PMID: 26911661
  7. These results imply daintain/AIF-1 triggers type 1 diabetes probably via arousing immune cells activation and induction of NO production in pancreas of NOD mice. PMID: 23022189
  8. AIF-1 was overexpressed in the liver of BALB/c mice infected with S. japonicum, and the interaction between AIF-1 and TNF-alpha or other cytokines played an important role in the pathogenesis and progression of hepatic schistosomiasis. PMID: 22150368
  9. Data suggest a strong association between AIF-1 expression, NF-kappaB activation, and development of experimental atherosclerosis. PMID: 21862018
  10. AIF-1 can induce IL-6 secretion on mononuclear cells and fibroblast chemotaxis. AIF-1 may accordingly provide an attractive new target for antifibrotic therapy in SSc as well as Scl GVHD. PMID: 21040744
  11. interferon gamma inducable and macrophage restricted expression requires Pu.1 PMID: 11861656
  12. This study demonstrated for the first time that allograft inflammatory factor-1(AIF-1) was expressed in uterus, and a correlation between AIF-1 production and fetal resorption in mice. PMID: 14506935
  13. These data indicate that AIF-1 mediates atherogenesis-initiated signaling and activation of macrophages. PMID: 16291819
  14. The molecular conformational change induced by Ca(2+)-binding of Iba1 is different from that found in the classical EF-hand proteins, which demonstrates that Iba1 has an unique molecular switching mechanism dependent on Ca(2+)-binding. PMID: 17011575
  15. AIF-1/Iba1 can be regarded a "pan-macrophage marker" because, except for alveolar macrophages, all subpopulations of macrophages examined express AIF-1/Iba1. PMID: 17874251
  16. allograft inflammatory factor 1 (AIF-1) expression results in increased signal transduction, neointimal formation, and vascular smooth muscle cell proliferation in injured mouse carotid arteries. PMID: 17991871
  17. These results suggest that enhanced AIF-1 expression leads to augmented incorporation of degenerated LDL by macrophages and promotes development of atherosclerotic vasculopathy. PMID: 18204784
  18. Iba1 was heavily expressed in the VM-M2, VM-M3 and RAW cells, but was mostly undetectable in the VM-NM1 cells and the AC cells. These findings support the microglial/macrophage origin of the metastatic VM-M2, VM-M3 tumor cells. PMID: 18398829
  19. AIF-1 is expressed in endothelial cells, macrophages and T cells in systemic sclerosis (SSc). PMID: 16868985

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Subcellular Location Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, phagocytic cup
Tissue Specificity Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages.
Database Links

KEGG: mmu:11629

STRING: 10090.ENSMUSP00000025257

UniGene: Mm.10747

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