Product Details

Target Name

angiopoietin-like 3

Associated names

Angptl3Angiopoietin-related protein 3 ELISA kit; Angiopoietin-like protein 3) [Cleaved into: ANGPTL3(17-224)] ELISA kit

Abbreviation

ANGPTL3

Uniprot No.

Q9R182

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

10.24 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse ANGPTL3 ELISA Kit was designed for the quantitative measurement of Mouse ANGPTL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 10.24 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: : 1:20

Review: I used the mouse Angiopoietin-related Protein ELISA Kit to detect the protein content in the serum of transgenic mice, setting up a model group, an administration group, and a negative control group. The detection results showed strong specificity, ensuring the accuracy and authenticity of the data.

By Anonymous

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Target Background

Function
(From Uniprot)

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity; the function seems to be specific for the feeding conditions. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids; the cleaved N-terminal domain is more efficient than the uncleaved proprotein. Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol. Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT. Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity.; Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration. May increase the motility of podocytes. Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS.

Gene References into Functions

The role of ANGPLT3 in controlling lipoprotein metabolism and risk of cardiovascular diseases is reviewed here. PMID: 29334984
ANGPTL8 has a functional LPL inhibitory motif, but only inhibits LPL and increases plasma TG levels in mice in the presence of ANGPTL3 PMID: 28413163
The data suggests that ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice. PMID: 29104012
Using in vitro ketosis model by glucose starvation, studied inhibition of ketosis by momilactone B. Found momilactone B could regulate the angiopoietin-like-3 (ANGPTL3)-lipoprotein lipase (LPL)pathway, and suppressed the expression of HMGCS2 through the increased expression of STAT5b. PMID: 27874312
This model suggests a general mechanism by which TAG trafficking is coordinated by lipasin, Angptl3 and Angptl4 at different nutritional statuses. PMID: 26687026
Inactivation of ANGPTL3 reduces hepatic VLDL-triglyceride secretion PMID: 25954050
The deletion of ANGPTL3 tremendously attenuates proteinuria and protects podocytes from injury in a mouse model of adriamycin-induced nephropathy. PMID: 25710887
ANGPTL3 has a role in regulating white adipose tissue energy homeostasis but not in liver PMID: 26305978
Data indicate that expression of Angptl3 in hematopoietic stem cell (HSC) through lentiviral transduction promoted HSC expansion. PMID: 25170927
Angptl3 could induce actin filament rearrangement, mainly in lamellipodia formation, and that this process was mediated by integrin alpha(V)beta-mediated FAK and PI3K phosphorylation and Rac1 activation. PMID: 24294595
Furin has a role as the primary in vivo convertase of ANGPTL3 and endothelial lipase in hepatocytes PMID: 23918928
ANGPTL8, a paralog of ANGPTL3 that arose through duplication of an ancestral DOCK gene, regulates postprandial TAG and fatty acid metabolism by controlling activation of its progenitor, and perhaps other ANGPTLs PMID: 23150577
Angptl3, as an extrinsic factor, thus supports the stemness of hematopoietic stem cells in the bone marrow niche. PMID: 20959605
ANGPTL3 expression is upregulated in puromycin-induced podocyte damage and is associated with the reduction of perlecan and agrin expression PMID: 20424482
a molecular connection between ANGPTL3, lipoprotein lipase, and proprotein convertases PMID: 20581395
ANGPTL3 to be capable of regulating the motility and permeability of podocytes and that the mechanism of ANGPTL3's regulation could be associated with the altered expression of nephrin. PMID: 20633534
Like ANGPTL4, ANGPTL3 inhibited nonstabilized LPL but not GPIHBP1-stabilized LPL PMID: 19542565
ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo PMID: 11877390
affects VLDL triglyceride clearance by interfering with LPL activity PMID: 12097324
hepatic Angptl3 has a role in hypertriglyceridemia associated with the treatment of LXR ligand PMID: 12672813
the cleavage of ANGPTL3 at two sites is important for the activation of ANGPTL3 in vivo PMID: 12909640
Expression of ANGPTL3 was enhanced in both insulin-deficient and -resistant diabetic states; results strongly suggest ANGPTL3 to play an important role in hyperlipidemia in diabetes. PMID: 15094378
Elevated ANGPTL3 by leptin- or insulin-resistance is attributed to increased plasma triglycerides and free fatty acid levels in obesity. PMID: 15336575
Differential regulation of Angptl3 and Angptl4 by sites of expression, nutritional status, and ligands of nuclear receptors may confer unique roles of each in lipoprotein metabolism. Angptl3 is a target gene of liver X receptor PMID: 15863837
Angptl3-deficiecy displayed hypotriglyceridemia with elevated postheparin plasma lipoprotein lipase, with greater effect in fed state. Deficiecy in both Angptl proteins had additive effect on plasma triglycerides with survival not past 2 months of age. PMID: 16081640
Angptl3 acts as an inhibitor of EL and may be involved in the regulation of plasma HDL cholesterol and HDL-PL levels in humans and rodents. PMID: 17110602
SE1 region of ANGPTL3 and ANGPTL4 functions as a domain important for binding LPL and inhibiting its activity in vitro and in vivo. PMID: 19318355

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Subcellular Location

Secreted. Cell projection, lamellipodium.

Tissue Specificity

Predominantly expressed in liver, weakly expressed in kidney and lung. Expressed in podocytes (at protein level). Expressed in hypothalamic neurons (at protein level). Expressed in bone marrow sinusoidal endothelial cells (at protein level).

Database Links

KEGG: mmu:30924

STRING: 10090.ENSMUSP00000030280

UniGene: Mm.28341

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Code	CSB-EL001711MO
Size	96T,5×96T,10×96T
Price	Request a Quote
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Mouse Angiopoietin-related protein 3(ANGPTL3) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background