| Description |
The ELISA kit CSB-EL002005MO is applied to quantitatively detect the content of the Arginase-1 (ARG1) in samples of serum, plasma, and tissue homogenates. The detection mechanism of this mouse ARG1 ELISA kit is based on the sandwich assay. The standards or samples are added to the microtiter plate wells pre-coated with ARG1 specific antibody. Biotin-labeled ARG1 antibody and HRP-avidin are successively pipped into the wells, forming the immune complex. The solution develops color after the addition of TMB substrate. The content of ARG1 can be quantitatively determined by measuring the optical density of color with a microplate reader. This ELISA kit has been verified with high sensitivity, excellent specificity, a precision of less than 10%, good correlation and linearity, and high recovery.
ARG1 is a cytosolic enzyme constitutively expressed in the liver cells. It is responsible for the hydrolysis of arginine to ornithine and urea and plays a major role in the hepatic urea cycle. The resulting polyamines are important for cell proliferation and clearance of protein degradation-produced toxins. ARG1 deprives nitric oxide (NO) synthase of its substrate and down-regulates NO synthesis. ARG1 deficiencies cause the autosomal disease argininemia that is featured by hyperammonemia. Studies have recently demonstrated that ARG1 is induced in alternatively activated macrophages and is involved in anti-inflammation, tumor immunity, tumor proliferation, metastasis, and immunosuppression-associated diseases. ARG1 may be expressed in the myeloid cells infiltrating tumors and is typically found in the majority of hepatocellular carcinomas. |
| Target Name |
arginase, liver |
| Alternative Names |
Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit |
| Abbreviation |
ARG1 |
| Uniprot No. |
Q61176 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
|
| Range % |
90-100 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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| Recovery |
| The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
93-96 |
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| EDTA plasma (n=4) |
96 |
89-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| mU/ml. |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.566 |
2.599 |
2.583 |
2.447 |
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| 10 |
2.165 |
2.199 |
2.182 |
2.046 |
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| 5 |
1.599 |
1.551 |
1.575 |
1.439 |
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| 2.5 |
0.899 |
0.880 |
0.890 |
0.754 |
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| 1.25 |
0.575 |
0.593 |
0.584 |
0.448 |
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| 0.625 |
0.292 |
0.299 |
0.296 |
0.160 |
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| 0.312 |
0.222 |
0.235 |
0.229 |
0.093 |
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| 0 |
0.134 |
0.137 |
0.136 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse ARG1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ARG1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
