Mouse Arginase-1(ARG1) ELISA kit

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Target Data

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Product Details

Target Name

arginase, liver

Alternative Names

, arginase 1|liver-type arginase|type I arginase

Abbreviation

ARG1

Uniprot No.

Q61176

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	89
	Range %	84-95
1:4	Average %	98
	Range %	93-104
1:8	Average %	97
	Range %	92-104

Recovery

The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	93-96
EDTA plasma (n=4)	96	89-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml.	OD1	OD2	Average	Corrected
20	2.566	2.599	2.583	2.447
10	2.165	2.199	2.182	2.046
5	1.599	1.551	1.575	1.439
2.5	0.899	0.880	0.890	0.754
1.25	0.575	0.593	0.584	0.448
0.625	0.292	0.299	0.296	0.160
0.312	0.222	0.235	0.229	0.093
0	0.134	0.137	0.136

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

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Target Data

Function	Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Subcellular Location	Cytoplasm, Cytoplasmic granule
Protein Families	Arginase family
Tissue Specificity	Expressed in macrophages (PubMed:7537672, PubMed:12193690, PubMed:19360123). Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s) (PubMed:27043409). Expressed in lung tumor-associated myeloid cells (PubMed:15313928). Expressed in lung t
Database Links	KEGG: mmu:11846 STRING: 10090.ENSMUSP00000020161 UniGene: Mm.154144

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Check Code：

Code	CSB-EL002005MO
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.