Mouse Arginase-1(ARG1) ELISA kit

Instructions
Code CSB-EL002005MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The ELISA kit CSB-EL002005MO is applied to quantitatively detect the content of the Arginase-1 (ARG1) in samples of serum, plasma, and tissue homogenates. The detection mechanism of this mouse ARG1 ELISA kit is based on the sandwich assay. The standards or samples are added to the microtiter plate wells pre-coated with ARG1 specific antibody. Biotin-labeled ARG1 antibody and HRP-avidin are successively pipped into the wells, forming the immune complex. The solution develops color after the addition of TMB substrate. The content of ARG1 can be quantitatively determined by measuring the optical density of color with a microplate reader. This ELISA kit has been verified with high sensitivity, excellent specificity, a precision of less than 10%, good correlation and linearity, and high recovery.

ARG1 is a cytosolic enzyme constitutively expressed in the liver cells. It is responsible for the hydrolysis of arginine to ornithine and urea and plays a major role in the hepatic urea cycle. The resulting polyamines are important for cell proliferation and clearance of protein degradation-produced toxins. ARG1 deprives nitric oxide (NO) synthase of its substrate and down-regulates NO synthesis. ARG1 deficiencies cause the autosomal disease argininemia that is featured by hyperammonemia. Studies have recently demonstrated that ARG1 is induced in alternatively activated macrophages and is involved in anti-inflammation, tumor immunity, tumor proliferation, metastasis, and immunosuppression-associated diseases. ARG1 may be expressed in the myeloid cells infiltrating tumors and is typically found in the majority of hepatocellular carcinomas.

Target Name arginase, liver
Alternative Names Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit
Abbreviation ARG1
Uniprot No. Q61176
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-100  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 98  
Range % 93-104  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 93-96  
EDTA plasma (n=4) 96 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml. OD1 OD2 Average Corrected  
20 2.566 2.599 2.583 2.447  
10 2.165 2.199 2.182 2.046  
5 1.599 1.551 1.575 1.439  
2.5 0.899 0.880 0.890 0.754  
1.25 0.575 0.593 0.584 0.448  
0.625 0.292 0.299 0.296 0.160  
0.312 0.222 0.235 0.229 0.093  
0 0.134 0.137 0.136    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse ARG1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled ARG1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Gene References into Functions
  1. Our studies provide proof-of-concept for gene-editing at the Arg1 locus and highlight the challenges that lie ahead to restore sufficient liver-based urea cycle function in patients with urea cycle disorders. PMID: 28566761
  2. This study shows for the first time that diabetes-induced increases in arginase 1 expression promotes endothelial cell senescence through the activation of p16INK4A and p53 signaling pathways. PMID: 29673160
  3. findings identified a novel function of the VEGFR1 signaling in avoiding over-expression of Arginase 1 potentially to maintain the proper innate immune response. PMID: 29110610
  4. Up-regulation of arginase 1 expression/activity in vascular endothelial cells has an integral role in diet-induced cardiovascular dysfunction and metabolic syndrome. PMID: 29048462
  5. Complete ablation of Arg1 in the lung affects mRNA abundance of arginine-transporting and -metabolizing genes, and pro-inflammatory genes, but not methacholine responsiveness or accumulation of inflammatory cells. PMID: 29183288
  6. Data suggest that Arg1 mRNA and protein expression in liver are significantly higher in obese mice (fed high-fat diet) than in control mice; hepatic Arg1 levels positively correlate with plasma Arg1 levels and negatively correlate with L-arginine bioavailability in plasma. Increased expression of hepatic Arg1 and reduced plasma L-arginine and NO(2)(-) may lead to vascular endothelial dysfunction even in early obesity. PMID: 29098611
  7. Data suggest that chronic hypoxia enhances HIF-2alpha stability, which causes increased arginase expression and dysregulates normal vascular NO homeostasis. PMID: 27432976
  8. high-fat/high-sucrose diet-induced visceral adipose tissue inflammation is mediated by endothelial cells-A1 expression/activity. PMID: 28835451
  9. Deletion of Arg1 in hematopoietic cells adversely affects blood leukocyte counts and increases foam cell formation. However, no effects on atherosclerosis could be demonstrated, indicating that hematopoietic Arg1 function is not a decisive factor in atherosclerotic plaque formation. PMID: 27998825
  10. diabetes-dependent increase in renal arginase-2 expression also requires arginase-1 expression in macrophages PMID: 28446459
  11. this study shows that mice lacking Arg1 in macrophages develop increased allergic contact hypersensitivity PMID: 28747341
  12. this study shows that Ron is expressed in a subpopulation of macrophages during chronic inflammation induced by obesity that exhibit a repair phenotype as determined by the expression of arginase 1 PMID: 27233965
  13. Arginase 1 was highly expressed by tumor-associated Gr1+ microglia and macrophages. PMID: 27936099
  14. This study uncovers synergistic activation of Arg1 by retinoic acid and IL-4 in M2 macrophages. PMID: 27166374
  15. this study shows that group 2 innate lymphoid cells selectively express arginase 1 and that this is critical for their bioenergetics, proliferation and function PMID: 27043409
  16. this study shows that c-Jun regulates the activation state of macrophages and promotes arthritis via differentially regulating cyclooxygenase-2 and arginase-1 levels PMID: 28298526
  17. deletion or TNF-mediated restriction of Arg1 unleashes the production of nitric oxide by NOS2, which is critical for pathogen control. PMID: 27117406
  18. Data indicate that transfected mouse arginase-1 (Arg-I) promotes endothelial senescence and inflammatory responses through eNOS-uncoupling in human umbilical vein endothelial cells (HUVEC cells). PMID: 28153047
  19. Arg1 is down-regulated during osteoclast differentiation and may negatively regulate osteoclast differentiation by regulating nitric oxide production. PMID: 26475291
  20. Results show that arginase 1 is abundantly present in the bone and bone marrow stromal cells and reveal the role of its deregulation in diabetes-induced bone complications. * HFHS diet induced Arginase activity and expression in bone and bone marrow. PMID: 26704078
  21. T Lymphocyte Inhibition by Tumor-Infiltrating Dendritic Cells Involves Ectonucleotidase CD39 but Not Arginase-1. PMID: 26491691
  22. The production of arginase-1 by tumor cells leads to an ineffective anti-tumor immune response. PMID: 24614600
  23. Arg1+ microglia are involved in Abeta plaque reduction during sustained, IL-1beta-dependent neuroinflammation PMID: 26538310
  24. findings suggest that increased IL-17A expression by macrophages in E7-expressing skin exposed to DNCB promotes arginase-1 induction and contributes directly to the observed hyperinflammation. PMID: 25720383
  25. Arginase 1 is crucially involved in Ang II-induced smooth muscle cell proliferation and arterial fibrosis and stiffness and represents a promising therapeutic target. PMID: 25807386
  26. FoxO4 activates Arg1 transcription in endothelial cells in response to MI, leading to downregulation of nitric oxide and upregulation of neutrophil infiltration to the infarct area. PMID: 26438688
  27. TAT fused to WW2/WW3 of Smurf2 could interfere with TGF-beta signaling and reduce ArgI gene expression. PMID: 25612247
  28. ARG1 activity may participate in the pathogenesis of lymphoproliferative and myeloproliferative disorders. PMID: 26363032
  29. data indicate that helminth coinfection induces arginase-1-expressing type 2 granulomas, thereby increasing inflammation and TB disease severity. PMID: 26571397
  30. Flow cytometric analysis of intrasplenic leukocytes exposed the presence of a transient population of activated neutrophils that correlated quantitatively with elevated ArgI levels in culture media PMID: 25966956
  31. Arginase 1 activity worsens lung-protective immunity against Streptococcus pneumoniae infection. PMID: 25789453
  32. Arg1 expression is decreased, and Arg2 expression is increased in the newborn congenital obstructive nephropathy and in the mouse model. PMID: 25205225
  33. Promoter region methylation was decreased at Arg1 in THC-induced myeloid-derived suppressor cells, which then expressed higher Arg1 levels. PMID: 25713087
  34. Arg1 plays a central role in the control of TB when NOS2 is rendered ineffective by hypoxia. PMID: 25201986
  35. arginase I activity is required for M2 macrophages mediated protection during DSS-induced colitis in PI3Kp110delta-deficient mice. PMID: 25124254
  36. the cross talk between HDAC4 and STAT6 is an important regulatory mechanism of Arg1 transcription in dendritic cells PMID: 25332236
  37. Diabetic mice exhibit elevated vascular Arg1 and impaired vascular endothelial and nitrergic function. PMID: 23977269
  38. A myeloid cell uptake of damaged retinal pigment epithelium or its derivatives as a mechanism generating VEGF (+) Arg-1(+) phenotype in vivo, is demonstrated. PMID: 23977372
  39. Our results demonstrate that HPV16.E7 protein enhances drug associated production of arginase-1 by myeloid cells and consequent inflammatory cellular infiltration of skin. PMID: 24732401
  40. Reduced arginase-1 activity in Arg1(fl/fl)/Tie2-Cre(tg/-) mice resulted in increased inflammatory response and nitric oxide production by NOS2. PMID: 24465919
  41. these data support the notion that PTEN contributes to cell fate decisions of macrophages exemplified by increased Arginase I expression. PMID: 25015834
  42. tumor myeloid cells inhibit the adaptive immune response after radiation therapy through expression of the enzyme arginase I PMID: 24992164
  43. neither induction of alternative activation nor expression of arginase1 are critical features of disease mediated by attenuated or virulent Francisella species. PMID: 24324751
  44. A kinesin heavy chain released by T. brucei induces IL-10 and arginase-1 through SIGN-R1 signaling in myeloid cells, which promotes early trypanosome growth and favors parasite settlement in the host. PMID: 24204274
  45. ATRA enhances both Arg-1 and iNOS expression in IFN-gamma-treated DCs, resulting in a tolerogenic phenotype. PMID: 24790153
  46. Murine primary macrophages treated with HDL showed increased gene expression for the M2 markers Arginase-1 (Arg-1) and Fizz-1 PMID: 23991225
  47. ILC2s are a novel source of Arg1 in resting tissue and during allergic inflammation. PMID: 23924659
  48. In preclinical murine models reduced Arg expression directly correlates with delayed healing of skin wounds. PMID: 23552798
  49. Our objective was to elucidate mechanisms involved in modulating arginase I induction by IL-4 in murine M2 macrophages PMID: 23913966
  50. wall shear stress is a key biomechanical regulator of arginase during atherosclerotic plaque formation and stability PMID: 23390893

Show More

Hide All

Subcellular Location Cytoplasm, Cytoplasmic granule
Protein Families Arginase family
Tissue Specificity Expressed in macrophages (PubMed:7537672, PubMed:12193690, PubMed:19360123). Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s) (PubMed:27043409). Expressed in lung tumor-associated myeloid cells (PubMed:15313928). Expressed in lung t
Database Links

KEGG: mmu:11846

STRING: 10090.ENSMUSP00000020161

UniGene: Mm.154144

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1