Mouse Arginase-1(ARG1) ELISA kit

Instructions
Code CSB-EL002005MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name arginase, liver
Alternative Names , arginase 1|liver-type arginase|type I arginase
Abbreviation ARG1
Uniprot No. Q61176
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-100  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 98  
Range % 93-104  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 93-96  
EDTA plasma (n=4) 96 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml. OD1 OD2 Average Corrected  
20 2.566 2.599 2.583 2.447  
10 2.165 2.199 2.182 2.046  
5 1.599 1.551 1.575 1.439  
2.5 0.899 0.880 0.890 0.754  
1.25 0.575 0.593 0.584 0.448  
0.625 0.292 0.299 0.296 0.160  
0.312 0.222 0.235 0.229 0.093  
0 0.134 0.137 0.136    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Subcellular Location Cytoplasm, Cytoplasmic granule
Protein Families Arginase family
Tissue Specificity Expressed in macrophages (PubMed:7537672, PubMed:12193690, PubMed:19360123). Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s) (PubMed:27043409). Expressed in lung tumor-associated myeloid cells (PubMed:15313928). Expressed in lung t
Database Links

KEGG: mmu:11846

STRING: 10090.ENSMUSP00000020161

UniGene: Mm.154144

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