Mouse Arginase-1(ARG1) ELISA kit

Instructions
Code CSB-EL002005MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name arginase, liver
Alternative Names Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit
Abbreviation ARG1
Uniprot No. Q61176
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-100  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 98  
Range % 93-104  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 93-96  
EDTA plasma (n=4) 96 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml. OD1 OD2 Average Corrected  
20 2.566 2.599 2.583 2.447  
10 2.165 2.199 2.182 2.046  
5 1.599 1.551 1.575 1.439  
2.5 0.899 0.880 0.890 0.754  
1.25 0.575 0.593 0.584 0.448  
0.625 0.292 0.299 0.296 0.160  
0.312 0.222 0.235 0.229 0.093  
0 0.134 0.137 0.136    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Gene References into Functions
  1. Our studies provide proof-of-concept for gene-editing at the Arg1 locus and highlight the challenges that lie ahead to restore sufficient liver-based urea cycle function in patients with urea cycle disorders. PMID: 28566761
  2. This study shows for the first time that diabetes-induced increases in arginase 1 expression promotes endothelial cell senescence through the activation of p16INK4A and p53 signaling pathways. PMID: 29673160
  3. findings identified a novel function of the VEGFR1 signaling in avoiding over-expression of Arginase 1 potentially to maintain the proper innate immune response. PMID: 29110610
  4. Up-regulation of arginase 1 expression/activity in vascular endothelial cells has an integral role in diet-induced cardiovascular dysfunction and metabolic syndrome. PMID: 29048462
  5. Complete ablation of Arg1 in the lung affects mRNA abundance of arginine-transporting and -metabolizing genes, and pro-inflammatory genes, but not methacholine responsiveness or accumulation of inflammatory cells. PMID: 29183288
  6. Data suggest that Arg1 mRNA and protein expression in liver are significantly higher in obese mice (fed high-fat diet) than in control mice; hepatic Arg1 levels positively correlate with plasma Arg1 levels and negatively correlate with L-arginine bioavailability in plasma. Increased expression of hepatic Arg1 and reduced plasma L-arginine and NO(2)(-) may lead to vascular endothelial dysfunction even in early obesity. PMID: 29098611
  7. Data suggest that chronic hypoxia enhances HIF-2alpha stability, which causes increased arginase expression and dysregulates normal vascular NO homeostasis. PMID: 27432976
  8. high-fat/high-sucrose diet-induced visceral adipose tissue inflammation is mediated by endothelial cells-A1 expression/activity. PMID: 28835451
  9. Deletion of Arg1 in hematopoietic cells adversely affects blood leukocyte counts and increases foam cell formation. However, no effects on atherosclerosis could be demonstrated, indicating that hematopoietic Arg1 function is not a decisive factor in atherosclerotic plaque formation. PMID: 27998825
  10. diabetes-dependent increase in renal arginase-2 expression also requires arginase-1 expression in macrophages PMID: 28446459
  11. this study shows that mice lacking Arg1 in macrophages develop increased allergic contact hypersensitivity PMID: 28747341
  12. this study shows that Ron is expressed in a subpopulation of macrophages during chronic inflammation induced by obesity that exhibit a repair phenotype as determined by the expression of arginase 1 PMID: 27233965
  13. Arginase 1 was highly expressed by tumor-associated Gr1+ microglia and macrophages. PMID: 27936099
  14. This study uncovers synergistic activation of Arg1 by retinoic acid and IL-4 in M2 macrophages. PMID: 27166374
  15. this study shows that group 2 innate lymphoid cells selectively express arginase 1 and that this is critical for their bioenergetics, proliferation and function PMID: 27043409
  16. this study shows that c-Jun regulates the activation state of macrophages and promotes arthritis via differentially regulating cyclooxygenase-2 and arginase-1 levels PMID: 28298526
  17. deletion or TNF-mediated restriction of Arg1 unleashes the production of nitric oxide by NOS2, which is critical for pathogen control. PMID: 27117406
  18. Data indicate that transfected mouse arginase-1 (Arg-I) promotes endothelial senescence and inflammatory responses through eNOS-uncoupling in human umbilical vein endothelial cells (HUVEC cells). PMID: 28153047
  19. Arg1 is down-regulated during osteoclast differentiation and may negatively regulate osteoclast differentiation by regulating nitric oxide production. PMID: 26475291
  20. Results show that arginase 1 is abundantly present in the bone and bone marrow stromal cells and reveal the role of its deregulation in diabetes-induced bone complications. * HFHS diet induced Arginase activity and expression in bone and bone marrow. PMID: 26704078
  21. T Lymphocyte Inhibition by Tumor-Infiltrating Dendritic Cells Involves Ectonucleotidase CD39 but Not Arginase-1. PMID: 26491691
  22. The production of arginase-1 by tumor cells leads to an ineffective anti-tumor immune response. PMID: 24614600
  23. Arg1+ microglia are involved in Abeta plaque reduction during sustained, IL-1beta-dependent neuroinflammation PMID: 26538310
  24. findings suggest that increased IL-17A expression by macrophages in E7-expressing skin exposed to DNCB promotes arginase-1 induction and contributes directly to the observed hyperinflammation. PMID: 25720383
  25. Arginase 1 is crucially involved in Ang II-induced smooth muscle cell proliferation and arterial fibrosis and stiffness and represents a promising therapeutic target. PMID: 25807386
  26. FoxO4 activates Arg1 transcription in endothelial cells in response to MI, leading to downregulation of nitric oxide and upregulation of neutrophil infiltration to the infarct area. PMID: 26438688
  27. TAT fused to WW2/WW3 of Smurf2 could interfere with TGF-beta signaling and reduce ArgI gene expression. PMID: 25612247
  28. ARG1 activity may participate in the pathogenesis of lymphoproliferative and myeloproliferative disorders. PMID: 26363032
  29. data indicate that helminth coinfection induces arginase-1-expressing type 2 granulomas, thereby increasing inflammation and TB disease severity. PMID: 26571397
  30. Flow cytometric analysis of intrasplenic leukocytes exposed the presence of a transient population of activated neutrophils that correlated quantitatively with elevated ArgI levels in culture media PMID: 25966956
  31. Arginase 1 activity worsens lung-protective immunity against Streptococcus pneumoniae infection. PMID: 25789453
  32. Arg1 expression is decreased, and Arg2 expression is increased in the newborn congenital obstructive nephropathy and in the mouse model. PMID: 25205225
  33. Promoter region methylation was decreased at Arg1 in THC-induced myeloid-derived suppressor cells, which then expressed higher Arg1 levels. PMID: 25713087
  34. Arg1 plays a central role in the control of TB when NOS2 is rendered ineffective by hypoxia. PMID: 25201986
  35. arginase I activity is required for M2 macrophages mediated protection during DSS-induced colitis in PI3Kp110delta-deficient mice. PMID: 25124254
  36. the cross talk between HDAC4 and STAT6 is an important regulatory mechanism of Arg1 transcription in dendritic cells PMID: 25332236
  37. Diabetic mice exhibit elevated vascular Arg1 and impaired vascular endothelial and nitrergic function. PMID: 23977269
  38. A myeloid cell uptake of damaged retinal pigment epithelium or its derivatives as a mechanism generating VEGF (+) Arg-1(+) phenotype in vivo, is demonstrated. PMID: 23977372
  39. Our results demonstrate that HPV16.E7 protein enhances drug associated production of arginase-1 by myeloid cells and consequent inflammatory cellular infiltration of skin. PMID: 24732401
  40. Reduced arginase-1 activity in Arg1(fl/fl)/Tie2-Cre(tg/-) mice resulted in increased inflammatory response and nitric oxide production by NOS2. PMID: 24465919
  41. these data support the notion that PTEN contributes to cell fate decisions of macrophages exemplified by increased Arginase I expression. PMID: 25015834
  42. tumor myeloid cells inhibit the adaptive immune response after radiation therapy through expression of the enzyme arginase I PMID: 24992164
  43. neither induction of alternative activation nor expression of arginase1 are critical features of disease mediated by attenuated or virulent Francisella species. PMID: 24324751
  44. A kinesin heavy chain released by T. brucei induces IL-10 and arginase-1 through SIGN-R1 signaling in myeloid cells, which promotes early trypanosome growth and favors parasite settlement in the host. PMID: 24204274
  45. ATRA enhances both Arg-1 and iNOS expression in IFN-gamma-treated DCs, resulting in a tolerogenic phenotype. PMID: 24790153
  46. Murine primary macrophages treated with HDL showed increased gene expression for the M2 markers Arginase-1 (Arg-1) and Fizz-1 PMID: 23991225
  47. ILC2s are a novel source of Arg1 in resting tissue and during allergic inflammation. PMID: 23924659
  48. In preclinical murine models reduced Arg expression directly correlates with delayed healing of skin wounds. PMID: 23552798
  49. Our objective was to elucidate mechanisms involved in modulating arginase I induction by IL-4 in murine M2 macrophages PMID: 23913966
  50. wall shear stress is a key biomechanical regulator of arginase during atherosclerotic plaque formation and stability PMID: 23390893
  51. Diabetes-induced increases in arginase I were involved in the diabetes-induced impairment of retinal blood flow by a mechanism involving vascular endothelial cell dysfunction. PMID: 23232640
  52. The consumption of L-arginine by arginase could lead to decreased production of nitric oxide from nitric oxide synthase, contributing to the pathogenesis of the colonic inflammation. PMID: 23215832
  53. In a classical model of atherosclerosis, aging induces an increase in level and activity of arginase I in the brains of ApoE-deficient mice. PMID: 22709928
  54. Data indicate that purified CD11b(+) cells obtained from the spleen of CpG-ODN+IFA-treated mice exhibited increased arginase (ARG) activity and ARG I expression along with an augmented [(3)H]-L-arginine uptake. PMID: 22083526
  55. Arthritogenic alphavirus infection drives a unique myeloid cell activation program in inflamed musculoskeletal tissues that inhibits virus clearance and impedes disease resolution in an arginase 1-dependent manner. PMID: 22972923
  56. Data indicate a major for arginase I in diabetes-induced vascular dysfunction. PMID: 22058149
  57. Toxoplasma gondii ROP16-dependent arginase-1 induction limits in vivo infection. PMID: 21931552
  58. Data show that progesterone not only down-regulates inducible nitric oxide synthase 2 (iNOS) activity in macrophages but also arginase activity. PMID: 21977998
  59. These data show that arginase is functional in the cornea and contributes to the immune privilege of the eye, and that modulation of arginase contributes to graft survival. PMID: 21805470
  60. These data demonstrate for the first time the role of TLR-4 in mediating the effects of L. mexicana promastigotes on MAP kinase activation, up-regulation of COX-2, iNOS as well as arginase-1 expression in macrophages PMID: 21664694
  61. Ron induces Arg1 expression in macrophages through a previously uncharacterized AP-1 site in the Arg1 promoter PMID: 21810604
  62. Arg1 is implicated in atherosclerosis regression; LXRalpha is a novel regulator of Arg1 and IRF8 in macrophages. PMID: 21757649
  63. enhanced IL-4-Ralpha expression by IL-10 might markedly amplify IL-4-mediated arginase 1 signaling and provide a possible mechanism for synergistic induction of arginase 1 PMID: 21413004
  64. Arginase is upregulated following environmental exposures in murine models of asthma, and contributes to the pollution-induced exacerbation of airways responsiveness. PMID: 21291525
  65. Mycobacteria-infected macrophages produced soluble factors, including interleukin-6 (IL-6), IL-10, and granulocyte colony-stimulating factor (G-CSF), that induced expression of Arg1 in an autocrine-paracrine manner. PMID: 20716764
  66. Extracellular activation of arginase-1 decreases enterocyte inducible nitric oxide synthase activity during systemic inflammation. PMID: 19713467
  67. results suggest that the level of TNF expression determines arginase expression in endothelial cells during myocardial I/R, which is one of the mechanisms by which TNF compromises coronary endothelial function in reperfusion injury PMID: 20091314
  68. macrophage-derived Arg I protects hosts against excessive tissue injury caused by worm eggs during acute schistosomiasis by suppressing IL-12/IL-23p40 production PMID: 20483789
  69. Increased capacity to upregulate arginase in younger mice results in a more permissive environment for parasite growth, increased disease severity and pathology. PMID: 18478052
  70. In a pulmonary schistosomiasis model, Arg-1 expression and activity influence the pathogenesis of granuloma formation and correlate with a Th2 response and maximum egg-induced pathology. PMID: 11714822
  71. arginase is involved in muscarinic receptor activation PMID: 12011984
  72. Arginase I expression in mouse embryonic development PMID: 12049781
  73. Arg1 increases superoxide production in myeloid cells through a pathway that likely utilizes the reductase domain of inducible NO synthase (iNOS) and that requires superoxide and induction by IL-4 for Arg1-dependent suppression of T cell function. PMID: 12496409
  74. induced in bleomycin-induced pulmonary fibrosis in mice. PMID: 12679322
  75. Gene expression of arginase I and II in pathogenesis of asthma. PMID: 12813022
  76. Upon injury, arginase-1 and -2 mRNA, protein, and activity were strongly induced reaching a maximum between day 3 and day 7 postwounding in diabetic mice PMID: 14675208
  77. Arginase I mRNA levels and enzyme activity in macrophages from Trypanosoma brucei brucei-infected mice are higher in susceptible BALB/c mice compared with resistant C57BL/6 mice. PMID: 15128819
  78. Th2-inducible arginase I expression in macrophages is controlled by an enhancer that lies -3 kb from the basal promoter PMID: 15187136
  79. The role of arginase in the Citrobacter rodentium model of colitis was studied. Arginase was induced in the colon during the infection. Arginase is protective by enhancing the generation of polyamines in addition to competitive inhibition of iNOS. PMID: 15265947
  80. C/EBPbeta has a key role in the modulation of macrophage arginase I expression in vivo and in vitro PMID: 15665733
  81. Elucidation of the mechanisms involved in regulation of arginase I transcription may provide a basis for developing strategies to modulate arginase expression in Th2 cytokine-predominant diseases. PMID: 15922518
  82. arginase I induction is a marker of disease in leishmaniasis PMID: 16113329
  83. Arginase I in myeloid suppressor cells is induced by COX-2 in lung carcinoma. PMID: 16186186
  84. upregulation of arginase activity by Th2 cytokines during xenograft rejection limits the bioavailability of L-arginine for the inducible nitric oxide synthase PMID: 16794538
  85. suggest an important role for metabolism of l-arginine by arginase I in the modulation of IL-13-induced AHR and identify a potential pathway distal to cytokine receptor interactions for the control of IL-13-mediated bronchoconstriction in asthma. PMID: 17015747
  86. Data suggest a regulatory mechanism where NF-kappaB is controlled through arginase-dependent regulation of nitric oxide levels, which may impact on chronic inflammatory diseases that are accompanied by NF-kappaB activation and upregulation of arginases. PMID: 17218616
  87. Neither the overexpression nor the deficiency of MKP-1 had any substantial effect on the expression of the arginases. PMID: 17442735
  88. cAMP-dependent activation of ARG1 expression is mediated by PKAI and requires histone deacetylation. PMID: 17577214
  89. Results suggest that endogenous arginase-derived ornithine may not directly contribute to polyamine homeostasis. PMID: 17686999
  90. ArgI is a key marker of the alternative program triggered by PPAR in macrophages. PMID: 18323470
  91. The expression of inducible nitric oxide synthase (iNOS) and arginase as markers of classical and alternative activation, respectively, in heart tissue during in vivo Chagas disease are reported. PMID: 18473687
  92. Murine ovarian cancer vascular leukocytes require arginase-1 activity for T cell suppression PMID: 18824264
  93. The blockage and silencing of PD-1, CTLA-4 or both PD-1 and CTLA4 molecules could significantly reduce arginase I activity and expression induced with tumor-associated factor. CD80 deficiency also decreased the arginase I expression and activity. PMID: 18828017
  94. Specific elimination of Arg1 in macrophages favored host survival during T. gondii infection and decreased lung bacterial load during tuberculosis infection PMID: 18978793
  95. NOS2 knockout mice are more sensitive to ovalbumin-induced airway inflammation and these findings are strongly correlated with increased expression of arginase I/II. PMID: 19027033
  96. In the acute murine model of allergic airway inflammation, augmentation of ARG1 expression was similarly the most dramatic change in protein expression; ARG2, NOS1, NOS2, and agmatinase were also increased, whereas NOS3 expression was decreased PMID: 19286931
  97. Arg1-expressing macrophages function as suppressors rather than inducers of Th2-dependent inflammation and fibrosis. PMID: 19360123
  98. Arginase I played a major role in the suppression of T cell response by regulatory DCs induced by 3LL lung cancer PMID: 19414774
  99. these data provide the first evidence that arginase is involved in the pathogenesis of an ocular disease. PMID: 19590038
  100. Alternatively-activated macrophages function as suppressor cells by an Arg1 dependent mechanism PMID: 19360123

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Subcellular Location Cytoplasm, Cytoplasmic granule
Protein Families Arginase family
Tissue Specificity Expressed in macrophages (PubMed:7537672, PubMed:12193690, PubMed:19360123). Expressed in precursor and mature group 2 innate lymphoid cells (ILC2s) (PubMed:27043409). Expressed in lung tumor-associated myeloid cells (PubMed:15313928). Expressed in lung t
Database Links

KEGG: mmu:11846

STRING: 10090.ENSMUSP00000020161

UniGene: Mm.154144

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