Mouse Aromatase(ARO) ELISA Kit

Code CSB-E14312m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
cytochrome P450, family 19, subfamily A, polypeptide 1
Alternative Names
Cyp19a1 ELISA kit; Arom ELISA kit; Cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
Abbreviation
CYP19A1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 95-106  
1:2 Average % 89  
Range % 84-93  
1:4 Average % 93  
Range % 89-96  
1:8 Average % 98  
Range % 93-104  
Recovery
The recovery of mouse ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 92 86-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.258 2.112 2.185 2.009  
200 1.644 1.695 1.670 1.494  
100 1.028 1.112 1.070 0.894  
50 0.588 0.604 0.596 0.420  
25 0.334 0.358 0.346 0.170  
12.5 0.249 0.257 0.253 0.077  
6.25 0.214 0.226 0.220 0.044  
0 0.181 0.171 0.176    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CYP19A1 ELISA Kit was designed for the quantitative measurement of Mouse CYP19A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Gene References into Functions
  1. This study demonstrated that Aromatase expression in spinal cord and medulla in sex difference. PMID: 28649695
  2. These studies suggest an association between adiposity, aromatase estrogenic capacity and atrial arrhythmogenicity. PMID: 28822806
  3. Increased adipose tissue aromatase activity reduces adipose tissue inflammation and improves insulin sensitivity in male mice. PMID: 28655716
  4. Aromatase-knockout (ArKO) mice cannot synthesize estrogens in vivo. Male ArKO mice developed hepatic steatosis accompanied by high testosterone levels. PMID: 28341248
  5. results show that the Aha1-Hsp90-PKM2/HIF-1alpha axis mediates the induction of aromatase in Li-Fraumeni Syndrome. PMID: 27467582
  6. aromatase could modulate pituitary LH-positive cells in males through local estradiol synthesis. PMID: 27160809
  7. Findings indicate that sex chromosome factors determine sex differences in aromatase expression in the stria terminalis and in the anterior amygdaloid area of mouse embryos. PMID: 26231585
  8. Aromatase plays an important role in the survival of metastatic ERalpha breast cancer cells by suppressing anoikis. PMID: 25837259
  9. aromatase may play a key role in the pathogenesis of Sjogren syndrome-like lesions by controlling the target organ and adipose tissue-associated macrophage; increased MPC1 protein expression has a role in adiposity PMID: 25447050
  10. The protective effect of female gender on abdominal aortic aneurysms is due to estrogen synthesis and requires the presence of both ovarian and extragonadal/peripheral aromatase. PMID: 24582702
  11. Male Aromatase knockout mice develop hepatic glucose intolerance. PMID: 24520329
  12. Cerebrovascular endothelial aromatase plays an important and sexually dimorphic role in cerebrovascular function and that aromatase inhibitors in clinical use may have cardiovascular consequences in both males and females. PMID: 24508640
  13. CYP19A1 gene is driven by promoters PII, I.4, and I.3 in the seminal vesicles, and by promoters PII and I.4 in the urinary bladder. PMID: 23239821
  14. Lhx2 is involved in the transcriptional regulation of aromatase in the rodent brain. PMID: 22734700
  15. Glucocorticoids alter aromatase expression in the hypothalamus and might regulate gonadotropin pulses and the menstrual cycle. PMID: 22705581
  16. Aromatase-deficient mice displayed increased cortical neuronal density and occasional cortical heterotopias PMID: 22426781
  17. Data show that the human aromatase gene can be expressed via its native promoters in a wide variety of mouse tissues and in a distribution pattern nearly identical to that of humans. PMID: 22508516
  18. expression level of estrogen receptors and aromatase in testis during aging PMID: 22366072
  19. investigation of role of aromatase in disease and inflammatory processes: Characterization of aromatase and apolipoprotein E double knockout mice as model of metabolic syndrome X. PMID: 22185842
  20. Impact of ovarian sex steroids on ovulation and ovulatory gene induction in aromatase-null mice. PMID: 22147013
  21. in the acute ischemia/reperfusion setting, more robust functional recovery, reduced cellular injury, and modified cardiomyocyte Ca(2+) handling was observe in aromatase-deficient hearts PMID: 22028441
  22. An organizational effect of estradiol during a critical period of development on the expression of biological rhythms. PMID: 21816154
  23. Mechanisms involved in amphetamine-induced c-fos expression are altered in aromatase knockout mice. PMID: 21093158
  24. Obesity is associated with inflammation and elevated aromatase expression in the mouse mammary gland PMID: 21372033
  25. The sexual differentiation of kisspeptin neuronal number was lost in aromatase knockout mice PMID: 19945459
  26. The study provides the first in vivo evidence linking the LH receptor, the EGFR, and ERK1/2 as sequential components of a pathway that regulates ovarian Cyp19a1 expression. PMID: 20093417
  27. Liver mitochondria isolated from ArKO mice displayed increased activity of the mitochondrial respiratory complex IV and were less prone to undergo cyclosporin A-sensitive mitochondrial permeability transition induced by calcium loading. PMID: 20194728
  28. FXR is able to compete with steroidogenic factor 1 in binding to a common sequence present in the aromatase promoter region interfering negatively with its activity. PMID: 20026603
  29. Data describe the effects of genistein and three synthetic estrogen receptor alpha/beta agonists in aromatase knockout mice by two routes of administration. PMID: 19686804
  30. Aromatase-deficient (ArKO) mice accumulate excess adipose tissue. PMID: 11850201
  31. Alternations in hepatic expression of fatty-acid metabolizing enzymes in ArKO mice and their reversal by the treatment with 17beta-estradiol or a peroxisome proliferator. PMID: 11850202
  32. Aromatase overexpression transgenic mice model: cell type specific expression and use of letrozole to abrogate mammary hyperplasia without affecting normal physiology. PMID: 11850204
  33. Bone phenotype of the aromatase deficient mouse. PMID: 11850207
  34. The ovarian phenotype of the aromatase knockout (ArKO) mouse. PMID: 11850223
  35. Characterization and purification of a protein binding to the cis-acting element for brain-specific exon 1 of the mouse aromatase gene. PMID: 11850232
  36. REVIEW: effects of depletion of endogenous estrogens in the aromatase deficient mouse (ArKO) with respect to spermiogenesis, development of obesity, and development of osteoporosis PMID: 12160996
  37. Inhibition of p-450 aromatase prevents feminisation and induces protection during cysticercosis PMID: 12350373
  38. The mouse Cyp19 gene includes three different promoters that specifically direct expression in ovary, testis, and brain. PMID: 12604651
  39. Induction of Fos in the accessory olfactory system by male odors persists in female mice with a null mutation of the aromatase (cyp19) gene. PMID: 12725902
  40. Forebrain Fos responses to reproductively related chemosensory cues in aromatase knockout mice. PMID: 12754080
  41. In male, but not female, aromatase knockout mice, there was an age-related reduction of prepulse inhibition of acoustic startle. PMID: 12884966
  42. aromatase knockout females presented with elevated serum cholesterol; conversely, aromatase knockout males had elevated hepatic cholesterol levels. PMID: 12933663
  43. These findings indicate that the biosynthetic enzyme P450 aromatase is key to endogenous neuroprotection in females and suggest that enhancing local, nongonadal estrogen formation could have therapeutic implications in cerebral ischemic neuropathology PMID: 14507969
  44. Review confirms the findings of aromatase neuroprotective effect in reactive astrocytes in vitro through use of aromatase knockout mice, which show enhanced hippocampal neurodegeneration in response to neurotoxins. PMID: 14993062
  45. aromatase activity is critical for normal autonomic control of the heart and, hence, for reducing the deleterious effects of high BP variability. PMID: 15178650
  46. Oestrogen-deficient female aromatase knockout (ArKO) mice exhibit depressive-like symptomatology. PMID: 15245494
  47. PGF(2alpha) decreases luteal P450arom mRNA levels at the end of pregnancy in rodents by inhibiting cyp19 expression. PMID: 15271885
  48. The aromatase immunostaining was found in cryptorchid-KE Leydig cells. PMID: 15823796
  49. Aromatase transgenic mice show increased levels of the estrogen receptor alpha and beta (ERalpha, ERbeta) suggesting that this receptor may play an important role in the initiation of estrogen-mediated mammary hyperplasia observed in these mice. PMID: 15955696
  50. p450 aromatase may have a role in male infertility associated with chronic inflammation in the testis PMID: 16306085

Show More

Hide All

Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X