Mouse Bone marrow proteoglycan(PRG2) ELISA kit

Code CSB-EL018670MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
Alternative Names
Prg2 ELISA Kit; Mbp-1 ELISA Kit; Bone marrow proteoglycan ELISA Kit; BMPG ELISA Kit; Proteoglycan 2) [Cleaved into: Eosinophil granule major basic protein ELISA Kit; EMBP ELISA Kit; MBP)] ELISA Kit
Abbreviation
PRG2
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.9 ng/mL-250 ng/mL
Sensitivity
0.98 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %94
Range %89-97
1:10Average %103
Range %98-107
1:20Average %90
Range %84-94
1:40Average %98
Range %92-102
Recovery
The recovery of mouse PRG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9488-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2502.772 2.664 2.718 2.597
1252.026 2.076 2.051 1.930
62.51.218 1.200 1.209 1.088
31.250.677 0.654 0.666 0.545
15.60.392 0.387 0.390 0.269
7.80.299 0.287 0.293 0.172
3.90.208 0.203 0.206 0.085
00.122 0.120 0.121
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PRG2 ELISA Kit was designed for the quantitative measurement of Mouse PRG2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 ng/mL-250 ng/mL and the sensitivity is 0.98 ng/mL.

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Target Background

Function
(From Uniprot)
Cytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions.
Gene References into Functions
  1. data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets. PMID: 27641493
  2. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
  3. major basic protein decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases. PMID: 16227527
  4. Host protection against Brugia pahangi infections was unimpaired in mice deficient in major basic protein 1. PMID: 16299347
  5. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice.(major basic protein 1) PMID: 16926417
  6. Eosinophil-derived MBP-1 plays a significant role in regulating muscular dystrophy by attenuating the cellular immune response and promoting tissue fibrosis that can eventually contribute to increased mortality. PMID: 18430716

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Subcellular Location
Cytoplasmic granule.
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