Recombinant Mouse Bone marrow proteoglycan (Prg2)

Code CSB-YP723430MO
MSDS
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Source Yeast
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Code CSB-EP723430MO
MSDS
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Source E.coli
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Code CSB-EP723430MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723430MO
MSDS
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Source Baculovirus
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Code CSB-MP723430MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prg2
Uniprot No.
Alternative Names
Prg2; Mbp-1; Bone marrow proteoglycan; BMPG; Proteoglycan 2) [Cleaved into: Eosinophil granule major basic protein; EMBP; MBP)]
Species
Mus musculus (Mouse)
Expression Region
17-223
Target Protein Sequence
LHLS SETSDSKSPL MDENLPRDAE ISGPEGEECP PGEELMPLEG EKEEGSGSEG VPGDEGAVSG QDVTDVDLQC PKEEDTTSLM GDSGCKTCRY LLVRRAECFD KAQSVCRRCY RGTLASIHSF SVNFGIQSAV RGINQGQVWI GGRIKGWGRC KRFRWVDGSS WNFAYWAAGQ PCPGGGRCVT LCTQGGHWRL SHCVKRRPFI CSY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions.
Gene References into Functions
  1. data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets. PMID: 27641493
  2. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
  3. major basic protein decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases. PMID: 16227527
  4. Host protection against Brugia pahangi infections was unimpaired in mice deficient in major basic protein 1. PMID: 16299347
  5. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice.(major basic protein 1) PMID: 16926417
  6. Eosinophil-derived MBP-1 plays a significant role in regulating muscular dystrophy by attenuating the cellular immune response and promoting tissue fibrosis that can eventually contribute to increased mortality. PMID: 18430716

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Subcellular Location
Cytoplasmic granule.
Database Links
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