Mouse C-type lectin domain family 18 member A(CLEC18A) ELISA kit

Code CSB-EL005521MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CLEC18A ELISA Kit was designed for the quantitative measurement of Mouse CLEC18A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name C-type lectin domain family 18, member A
Alternative Names Clec18a ELISA kit; Mrcl ELISA kit; MrlpC-type lectin domain family 18 member A ELISA kit; Mannose receptor-like protein ELISA kit
Abbreviation CLEC18A
Uniprot No. Q7TSQ1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLEC18A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-89
1:2 Average % 95
Range % 91-99
1:4 Average % 86
Range % 83-89
1:8 Average % 98
Range % 94-103
Recovery
The recovery of mouse CLEC18A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-94
EDTA plasma (n=4) 102 98-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.546 2.500 2.523 2.402
10 1.947 2.010 1.979 1.858
5 1.419 1.368 1.394 1.273
2.5 0.831 0.842 0.837 0.716
1.25 0.525 0.536 0.531 0.410
0.625 0.357 0.371 0.364 0.243
0.312 0.267 0.251 0.259 0.138
0 0.122 0.120 0.121  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Gene References into Functions
  1. Dendritic cells use the mannose receptor for endocytosis of a particular antigen type (soluble ovalbumin) intended for cross-presentation. PMID: 16709836
Subcellular Location Secreted.
Database Links

UniGene: Mm.135495

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