Mouse Calcitonin,CT ELISA Kit

Code CSB-E05133m
Size 96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Target Name calcitonin
Alternative Names CALCA Calcitonin
Abbreviation CALCA
Uniprot No. P70160
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %84
Range %80-88
1:2Average %95
Range %91-99
1:4Average %86
Range %83-89
1:8Average %97
Range %92-102
The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-93
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.026 2.068 2.047 1.945  
400 1.412 1.465 1.439 1.337  
200 0.908 0.935 0.922 0.820  
100 0.568 0.534 0.551 0.449  
50 0.346 0.326 0.336 0.234  
25 0.269 0.268 0.269 0.167  
12.5 0.189 0.198 0.194 0.092  
0 0.101 0.103 0.102    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Subcellular Location Secreted.
Protein Families Calcitonin family
Database Links

STRING: 10090.ENSMUSP00000032907

UniGene: Mm.4361

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