Mouse Calcitonin,CT ELISA Kit

Code CSB-E05133m
Size 96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
calcitonin
Alternative Names
CALCA Calcitonin
Abbreviation
CALCA
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %80-88
1:2Average %95
Range %91-99
1:4Average %86
Range %83-89
1:8Average %97
Range %92-102
Recovery
The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-93
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.026 2.068 2.047 1.945  
400 1.412 1.465 1.439 1.337  
200 0.908 0.935 0.922 0.820  
100 0.568 0.534 0.551 0.449  
50 0.346 0.326 0.336 0.234  
25 0.269 0.268 0.269 0.167  
12.5 0.189 0.198 0.194 0.092  
0 0.101 0.103 0.102    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

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Target Background

Function
(From Uniprot)
Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Subcellular Location
Secreted.
Protein Families
Calcitonin family
Database Links
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