Mouse Catalase (CAT) ELISA Kit

Instructions
Code CSB-E14190m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name catalase
Alternative Names Cat ELISA kit; Cas-1 ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
Abbreviation CAT
Uniprot No. P24270
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 87  
Range % 82-93  
1:2000 Average % 93  
Range % 84-99  
1:4000 Average % 98  
Range % 94-106  
1:8000 Average % 93  
Range % 86-98  
Recovery
The recovery of mouse CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.403 2.453 2.428 2.291  
500 2.178 2.196 2.187 2.050  
250 1.745 1.787 1.766 1.629  
125 1.217 1.309 1.263 1.126  
62.5 0.761 0.778 0.770 0.633  
31.2 0.482 0.499 0.491 0.354  
15.6 0.312 0.333 0.323 0.186  
0 0.138 0.136 0.137    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
Gene References into Functions
  1. It has been shown that genetic ablation of Nrf2 abolishes an adaptive muscle NQO1 activity and catalase induction. PMID: 29402993
  2. Catalase overexpression modulates metabolic parameters in a new 'stress-less' leptin-deficient mouse model. PMID: 28645653
  3. homocysteine reacts with native catalase and/or redox-active transition metal ions to generate thiyl radicals that mediate compound II formation, a temporarily inactive state of the enzyme. PMID: 27848965
  4. Catalase ameliorates diabetes-induced cardiomyopathy through reduced RelA-mediated transcription of BECN1. PMID: 28643395
  5. Mitochondrial catalase induces neoplastic cell transformation through nucleolin-dependent Cox-2 mRNA stabilization. PMID: 29097213
  6. Moderate MnSOD and/or catalase overexpression in desmin-null hearts leads to a marked decrease in intracellular reactive oxygen species, ameliorates mitochondrial and other ultrastructural defects, minimizes myocardial degeneration and leads to a significant improvement of cardiac function. PMID: 28629836
  7. Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. PMID: 27840320
  8. Mice devoid of catalase develop an obese, pre-diabetic phenotype and provide compelling evidence for catalase (or its products) being integral in metabolic regulation. PMID: 27939935
  9. Data indicate that alpha-syntrophin plays an important role in the regulation of oxidative stress from endogenously generated reactive oxygen species (ROS) during myoblast differentiation by modulating the protein stability of catalase. PMID: 28485891
  10. Overexpression of catalase reduced the concentrations of microsomal benzo(a)pyrene phenols and diols/diones by ~45 and 95%, respectively. PMID: 27607467
  11. Results indicate that mitochondrial catalase likely functions to preclude the formation of high levels of H2O2 without perturbing redox-dependent signaling. PMID: 27614223
  12. Age strongly interacts with catalase targeted to the mitochondria, consistent with antagonistic pleiotropy in the reverse of the typical direction. PMID: 27061426
  13. data suggest that catalase is required not only to scavenge reactive oxygen species, but also to protect DNA from oxidative damage during meiotic maturation in mouse oocytes. PMID: 27160095
  14. These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging. PMID: 27221506
  15. catalase protects mouse hearts against diabetic cardiomyopathy, partially by suppressing NF-kappaB-dependent inflammatory responses and associated protein nitration. PMID: 26456065
  16. Hypoxia-generated red blood cells have low catalase and are preferentially destroyed. PMID: 26017143
  17. The study shows how maturation of active catalase can be influenced by nitric oxide, S-nitrosylated GAPDH, and thioredoxin-1, and how maturation may become compromised in inflammatory conditions such as asthma. PMID: 25659933
  18. Data suggest that embryonic catalase is a determinant of risk for EtOH embryopathies. PMID: 26074427
  19. exposure to either 14- or 28-day chronic stress resulted in a depressive-like syndrome, behavioural invigoration and aggression, and decreased activity of two major brain peroxidation enzymes, superoxide dismutase and catalase. PMID: 24786329
  20. The antioxidant catalase counteracts against high fat diet-induced cardiac geometric and functional anomalies possibly via an IKKbeta-AMPK-dependent restoration of myocardial autophagy. PMID: 24993069
  21. The SirT1 regulates the expression of several antioxidant genes in bovine aortic endothelial cells, including Mn superoxide dismutase, catalase, peroxiredoxins 3 and 5, thioredoxin 2, thioredoxin reductase 2, and uncoupling protein 2. PMID: 23461683
  22. The levels of protein carbonyls, reactive oxygen species, and catalase in the cerebral hemispheres of young and aged mice, were evaluated. PMID: 24338129
  23. The expression levels of catalase and SOD2 are decreased in astrocytes when SIRT1 is inhibited. PMID: 24565075
  24. Data indicate that transgenic mice over-expressing human SOD1 or catalase were protected from loss of plasma membrane integrity (LPMI) at early but not late periods of reperfusion. PMID: 23911407
  25. Data indicate no significant difference in CAT and CuZnSOD gene expression, but enzymatic activity of CuZnSOD and MnSOD significantly increased after sleep deprivation. PMID: 23360182
  26. Up-regulation of catalase caused by high-fat diet is designed to protect heart mitochondria from oxidative damage while not perturbing H2O2-mediated signaling. PMID: 23204527
  27. Antioxidant enzyme, catalase, reverses DNA damage response and cellular transdifferentiation in aortic valve sclerosis. PMID: 23241403
  28. Myocardial catalase expression protects against endotoxemic myocardial dysfunction in mice. PMID: 22902401
  29. catalase overexpression in renal proximal tubular cells ameliorated maternal diabetes-induced perinatal programming, mediated in part by triggering the Nrf2-HO-1 defense system. PMID: 22733796
  30. The increased expression of antioxidant catalase, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371
  31. The findings indicated that lower skin catalase activity in male mice may at least in part contribute to increased ultraviolet light B-induced generation of Gr-1+CD11b+ myeloid cells. PMID: 22030957
  32. endogenous catalase plays an important role in protecting the kidney from diabetic stress through maintaining peroxisomal and mitochondrial fitness. PMID: 22315314
  33. physiological venous catalase activity prevents exercise-induced up-regulation of endothelial nitric oxide synthase and extracellular superoxide dismutase PMID: 21129156
  34. Data suggest that embryonic catalase plays an important protective role, and both physiological and phenytoin-enhanced oxidative stress can be embryopathic. PMID: 21478259
  35. Chestnut inner shell extract treatment with ethanol decreased CYP2E1 expression and increased activities of catalase and superoxide dismutase. PMID: 21457746
  36. Targeting catalase within mitochondria of tumor cells and tumor stromal cells suppresses ROS-driven tumor progression and metastasis. PMID: 21605372
  37. The data indicate catalase levels is decreased in stem cell-like characteristics. PMID: 21347610
  38. catalase may rescue paraquat-induced myocardial geometric and functional alteration possibly by alleviating JNK-mediated endoplasmic reticulum stress PMID: 20937379
  39. There were no acute functional improvements with catalase overexpression before myocardial infarction, but sustained H(2)O(2), influenced by catalase expression, levels may be critical in directing remodeling and cardiac function at later time points. PMID: 20971939
  40. The effect of increased mtDNA mutations on age-dependent cardiac structural changes and functional disturbance using Polgm/m mice and the contribution of mtreactive oxygen species in these mice by crossing Catalase to Polgm/m mice, was expored. PMID: 20456298
  41. overexpression of catalase decreases oxidant injury in the proximal tubular cells reverses abnormalities due to enhanced generation of reactive oxygen species in the proximal tubule cells PMID: 20237455
  42. peroxiredoxin-2, glutathione peroxidase-1, and catalase have roles in erythrocyte oxidative metabolism PMID: 19969073
  43. we have demonstrated that forced catalase expression in the mitochondria enhances exercise performance. Our result provides a framework for further elucidating the underlying mechanism PMID: 19690612
  44. A single base pair difference in the Cas1 open reading frame between the C57BL/6J and DBA/2J strains results in Ala-117 in the D2 strain and threonine at this position in the B6 strain. PMID: 11821648
  45. Overexpression of catalase diminishes the pressor response to NE and Ang II by reducing H(2)O(2) production in the arterial wall. PMID: 12517675
  46. The regulation of this gene's expression in mouse muscle cells is dependent on the CCAAT-binding factor NF-Y. PMID: 12659863
  47. findings indicate that, in addition to stimulating catalase activity, c-Abl and Arg promote catalase degradation in the oxidative stress response PMID: 12950161
  48. overexpression of catalase delays or prevents alterations in phospholipid metabolism during glucocorticoid-induced apoptosis PMID: 14572898
  49. effect of IGFBP-2 overexpression on the activity of catalas in two malignant cell lines PMID: 14710363
  50. the role of catalase in antioxidant defense is dependent on the type of tissue and the model of oxidant-mediated tissue injury PMID: 15178682

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Subcellular Location Peroxisome
Protein Families Catalase family
Database Links

KEGG: mmu:12359

STRING: 10090.ENSMUSP00000028610

UniGene: Mm.4215

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