Mouse Chitinase-3-like protein 1(CHI3L1) ELISA kit

Instructions
Code CSB-EL005346MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chitinase 3-like 1 (cartilage glycoprotein-39)
Alternative Names Chi3l1 ELISA Kit; Brp39 ELISA Kit; Chil1 ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; BRP39 protein ELISA Kit; Breast regression protein 39 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; GP-39 ELISA Kit
Abbreviation CHI3L1
Uniprot No. Q61362
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.47 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 91
Range % 86-95
1:4 Average % 103
Range % 99-107
1:8 Average % 94
Range % 85-98
Recovery
The recovery of mouse CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
30 2.601 2.476 2.539 2.319
15 1.933 1.819 1.876 1.656
7.5 1.140 1.204 1.172 0.952
3.75 0.692 0.682 0.687 0.467
1.88 0.457 0.441 0.449 0.229
0.94 0.349 0.358 0.354 0.134
0.47 0.284 0.297 0.291 0.071
0 0.224 0.215 0.220  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Gene References into Functions
  1. Chi3l1 is a regulator of Th1 and CTL which could be a therapeutic target to enhance anti-tumor immunity. PMID: 29403003
  2. Nrf2/Keap1 system regulates VSMC apoptosis during neointimal formation, thereby inhibiting neointimal hyperplasia after a vascular injury. PMID: 27198666
  3. Knockdown of Chi3l1 in the arterial endothelium suppressed the development of atherosclerosis in amyloid precursor protein transgenic mice. PMID: 29344304
  4. CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
  5. CHI3L1 deficiency in donor splenocytes dramatically increased the severity of aGVHD through enhancing Tfh cell differentiation PMID: 28656528
  6. miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway PMID: 28303416
  7. The mouse model was used to validate the function of CHI3L1 in cancer metastasis in vivo PMID: 28143526
  8. CHI3L1-mediated cell proliferation/survival involves partial downregulation of the pro-apoptotic S100A9 protein that is highly expressed during the acute phase of colitis, by binding to the S100A9 receptor, RAGE (Receptor for Advanced Glycation End products). PMID: 26431492
  9. IL-18 is a potent stimulator of Chi3l1 and that Chi3l1 is an important mediator of IL-18-induced inflammatory, fibrotic, alveolar remodeling, and cytotoxic responses. PMID: 25955511
  10. CHI3L1 is induced during fungal infection, and it regulated antifungal innate immune responses in the cornea. PMID: 26238714
  11. Astrocytic expression of chitinase 3-like protein 1 limits the extent of both astrocytic and microglial/macrophage facets of neuroinflammation. PMID: 25377763
  12. Results do not support a role of BRP-39 in the pathogenesis of experimental autoimmune encephalitis. PMID: 26079949
  13. CHI3L1 was increased in plasma and brain tissue in experimental cerebral malaria, but targeted Chi3l1 deletion did not alter cytokine production or survival in this model. PMID: 25047113
  14. mechanism of BRP-39 in bleomycin-induced pulmonary fibrosis PMID: 24659093
  15. Sem7a and its receptors regulate Chi3l1 in pulmonary melanoma metastasis PMID: 25511377
  16. Data show that IL-1-induced transcriptin factor RelB/NF-kappa B p50 Subunit complex formation was further promoted by oncostatin M and that these complexes directly bound to the secreted protein cartilage glycoprotein 39 (YKL-40) promoter. PMID: 25681350
  17. These results indicate that catalytically inactive BRP-39 and Ym1 are constitutive genes in normal mouse lung. PMID: 25294623
  18. BRP-39 is involved in repressing the M1 proinflammatory phenotype in hyperoxia, thereby deactivating inflammatory responses in macrophages and preventing neonatal lung injury. PMID: 24347826
  19. Chi3l1 binds to IL-13Ralpha2, stimulates MAPK, Akt/PKB, Wnt signaling and regulates melanoma metastasis. PMID: 23972995
  20. Stat3 downstream gene product chitinase 3-like 1 is elevated in lung cancer PMID: 23613996
  21. We identified the macrophage-secreted chitinase-like protein Brp-39 as a critical component of this reparative response that serves to limit tubular cell apoptotic death via activation of Akt, improving animal survival after kidney ischemia/reperfusion. PMID: 23291472
  22. BRP-39-deficient mice demonstrate the importance of BRP-39 in modulating the severity of clinical experimental autoimmune encephalomyelitis and central nervous system neuroinflammation. PMID: 23041842
  23. Chitinase 3-like-1 is induced by Streptococcus pneumoniae and plays central roles in promoting bacterial clearance and mediating host tolerance. PMID: 22817986
  24. CHI3L1 up-regulates pro-inflammatory mediators, C-chemokine ligand 2, chemokine CX motif ligand 2 and matrix metalloproteinase-9 all of which contribute to tumor growth and metastasis; study shows that CHI3L1 plays a role in tumor progression PMID: 21866546
  25. YKL-40-mediated inhibition of cell differentiation and polarization in the presence of lactogenic hormones may represent its important function during mammary tissue involution. PMID: 21991364
  26. YKL-40/BRP-39 levels are increased in human and murine CF airway fluids PMID: 21949714
  27. The carbohydrate-binding region of CHI3L1 is critical in activating Akt signaling in colon epithelial cells, and the activation may be associated with the development of chronic colitis. PMID: 21546314
  28. breast regression protein-39 has a role in the pathogenesis of cigarette smoke-induced inflammation and emphysema PMID: 20656949
  29. BRP-39 is functionally important in house dust mite-induced inflammation. BRP-39 is a biomarker of cigarette smoke induced inflammation which is the byproduct of an IL-1 inflammatory pathway. PMID: 21473774
  30. pathogenic role in colitis, presumably by enhancing the adhesion and invasion of bacteria on/into colonic epithelial cells. PMID: 16472595
  31. Cloning, expression, characterization and crystallization of BRP39, a signalling glycoprotein expressed during mammary gland apoptosis PMID: 19041398
  32. BRP-39 deficient mice show diminished antigen-induced Th2 cell responses, rescued by YKL-40 administration. PMID: 19414556

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Subcellular Location Secreted, extracellular space, Cytoplasm, Endoplasmic reticulum
Protein Families Glycosyl hydrolase 18 family
Tissue Specificity Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.
Database Links

KEGG: mmu:12654

STRING: 10090.ENSMUSP00000080717

UniGene: Mm.38274

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