Mouse Cholecystokinin,CCK ELISA Kit

Instructions
Code CSB-E08115m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cholecystokinin
Alternative Names CckCholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8)] ELISA Kit
Abbreviation CCK
Uniprot No. P09240
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %105
Range %100-112
1:2Average %91
Range %85-97
1:4Average %90
Range %85-94
1:8Average %93
Range %89-97
Recovery
The recovery of mouse CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-93
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
20002.573 2.463 2.518 2.394
10001.956 1.825 1.891 1.767
5001.302 1.245 1.274 1.150
2500.804 0.841 0.823 0.699
1250.537 0.512 0.525 0.401
62.50.320 0.301 0.311 0.187
31.250.225 0.234 0.230 0.106
00.121 0.127 0.124
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Gene References into Functions
  1. Study shows that independent of the neurochemical content, cholecystokinin/type 1 cannabinoid receptor-expressing interneurons have similar physiological and morphological properties, providing an endocannabinoid-sensitive synaptic inhibition onto the amygdalar principal neurons. PMID: 28391401
  2. In summary, our study is the first to indicate that CCK/CCK-AR pathway is critical and protective against liver I/R injury. The activation of this pathway not only prevents hepatocellular apoptosis, but also reduces inflammatory response by suppressing NF-kappaB. PMID: 28521244
  3. CCK/GLP-1 play contributory roles in anorexia induction by trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol and neosolaniol. PMID: 28964791
  4. GPR120-induced incretin glucse-dependent insulinotropic polypeptide secretion is indirectly mediated by cholecystokinin. PMID: 28324023
  5. Medullary interstitial cells respond to body fluid expansion by CCK release for feedback regulation of the late proximal tubular reabsorption. PMID: 28298361
  6. results suggest that normal integration of CCK+ basket cells in cortical networks is key to support spatial coding in the hippocampus. PMID: 28394324
  7. PC7 has a critical role in normal processing of cholecystokinin in mouse brain PMID: 27923657
  8. These studies reemphasize the beneficial effects imparted by co-administration of obestatin and CCK8 and their potential use towards countering obesity. PMID: 27032885
  9. new information on the cell specific localization of NUCB2/nesfatin-1 in the intestinal mucosa, and a novel function for nesfatin-1 in modulating intestinal CCK and PYY expression and secretion PMID: 26920055
  10. Results showed that CCK is important for lipid transport and energy expenditure to control body weight in response to dietary lipid feeding PMID: 26171590
  11. SP1 results in a CCK response deficiency that may contribute to the increased meal size and overall hyperphagia in synphillin-1 transgenic mice PMID: 26569394
  12. active GLP-1 produced in the islet stimulates cholecystokinin production and secretion in a paracrine manner via cyclic AMP and CREB. PMID: 25984632
  13. Data (including data from studies using transgenic mice) suggest that enhanced Cck expression in pancreatic beta-cells protects these cells from the cell-withering effects of aging, apoptotic stress, and (streptozotocin-induced) diabetes type 2. PMID: 26394663
  14. total Ca(2+) mobilization evoked by CCK-8 was attenuated by a 30% in the presence of 100 microM melatonin compared with the responses induced by CCK-8 alone. PMID: 25084987
  15. Endogenous cholecystokinin is in part responsible for the development and progression of pancreatic cancer. PMID: 25058882
  16. octapeptide exerts a direct effect on T cells, which is dependent on cholecystokinin-2 receptor PMID: 24704498
  17. Data demonstrate that the ERK pathway is required for CCK-stimulated pancreatic adaptive growth. PMID: 25104499
  18. I cells in duodenum are enriched in expression of CCK and ghrelin. PMID: 25004095
  19. Data show the transcriptional activation of the cholecystokinin gene by DJ-1 through interaction of DJ-1 with RREB1 and the effect of DJ-1 on the cholecystokinin level. PMID: 24348900
  20. This study concluded that CCK is a mediator of dietary fat-associated pancreatic cancer, and it is also involved in the invasiveness of pancreatic tumors. PMID: 24817409
  21. CCK enhances cholesterol absorption by activation of a pathway involving CCK1R/CCK2R, Gbetagamma, PI3K, Akt, Rab11a, and NPC1L. PMID: 24692543
  22. mouse corneal afferent sensory neurons expressed CCK and GAST, and the CCK1R receptors. PMID: 24576871
  23. Gastric PAI-1 modulates vagal effects of cholecystokinin. PMID: 23816469
  24. ILDR1 regulates CCK release through a mechanism dependent on fatty acids and lipoproteins. PMID: 23863714
  25. hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
  26. CD36 is a major mediator of FA-induced release of CCK and secretin. These peptides contribute to the role of CD36 in fat absorption and to its pleiotropic metabolic effects. PMID: 23233532
  27. a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin PMID: 23064014
  28. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
  29. These data suggest that a mixture of guar gum and fructo-oligosaccharide can maintain its appetite suppressant effect in fatty media. PMID: 23054308
  30. Our data demonstrate that CCK expressed in the basolateral amygdala represents a key brain substrate for anxiogenic and depressant effects of the peptide. PMID: 22613736
  31. expression of CCK is increased in hippocampal pyramidal cells in mice with recurrent, spontaneous seizures PMID: 22155653
  32. Data suggest that CCK acts to increase glutamatergic transmission to the preproglucagon-positive neurons; this action appears to involve activation of alpha1-adrenergic receptors. PMID: 21885869
  33. Simotang enhances the gastrointestinal motility in chronically stressed mice by regulating the serum motilin level and the expression of cholecystokinin. PMID: 21472126
  34. Gene expressions of ghrelin, PYY, and CCK was increased in the gastrointestinal tract of the hyperphagic intrauterine growth restriction rat offspring. PMID: 21264794
  35. Cholecystokinin signalling is not involved directly in light-induced resetting of the suprachiasmatic nucleus or in regulating its function. PMID: 20731710
  36. Short-term DeltaFosB overexpression increases both Cck promoter activity and gene expression. PMID: 20226774
  37. Conjugated linoleic acid isoforms are particularly potent CCK secretagogues, which also boost intracellular stores of CCK. PMID: 20352619
  38. CCK is up-regulated by islet cells during obesity and functions as a paracrine or autocrine factor to increase beta-cell survival and expand beta-cell mass to compensate for obesity-induced insulin resistance. PMID: 20534724
  39. CCK is involved in regulating the metabolic rate and is important for lipid absorption and control of body weight in mice placed on a high-fat diet. PMID: 20117110
  40. Lack of CCK induces gallbladder hypomotility that prolongs the residence time of excess cholesterol in the gallbladder, leading to rapid crystallization and precipitation of solid cholesterol crystals. PMID: 19836465
  41. role of anorectic effects possibly by enhancing release of pancreatic amylin PMID: 12576089
  42. In whole area of hippocampus, NPY-, SOM- (somatostatin), CCK-, and VIP-positive neurons accounted for about 31%, 17%, 7%, and 8% of GABAergic neurons, respectively. PMID: 12746872
  43. Intense cholecystokinin immunoreactivity is found in the mossy fiber pathway (stratum lucidum and dentate hilus) and in the inner molecular layer of the dentate gyrus. PMID: 14643757
  44. Lack of gastrin impairs gastrin-enterochromaffin-like cell axis, whereas lack of gastrin and CCK impairs both hormonal pathways. In gastrin-CCK double-knockout mice, acid secretion is controlled by cholinergic vagal stimulation, normalizing acid output. PMID: 14762785
  45. In this review, tissue-specific processing of cholecystokinin precursor in brain and gut to a large extent can be explained by the roles of prohormone convertases 1 and 2. PMID: 15075450
  46. These results indicate that CCK provides an inhibitory influence on GnRH-1 neuronal migration, contributing to the appropriate entrance of these neuroendocrine cells into the brain, and thus represent the first report of a developmental role for CCK PMID: 15152034
  47. Psychological stress inhibits the small intestinal transit, probably by down-regulating CCK and up-regulating VIP expression in small intestine. PMID: 15655834
  48. Both gastrin and combined gastrin-cholecytokinin deficiency reduced the gastric IAPP and Peptide Yy expression. PMID: 16002530
  49. prohormone convertase 2 is important for cholecystokinin processing PMID: 16174778
  50. results provide the first direct evidence that prohormone convertase 5 (PC5) is involved in CCK processing PMID: 16266771

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Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Database Links

KEGG: mmu:12424

STRING: 10090.ENSMUSP00000035120

UniGene: Mm.2619

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