Mouse Chordin-like protein 1(CHRDL1) ELISA kit

Code CSB-EL005378MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
chordin-like 1
Alternative Names
Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
Abbreviation
CHRDL1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-95
1:2Average %101
Range %97-106
1:4Average %98
Range %92-103
1:8Average %86
Range %82-89
Recovery
The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)8985-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.377 2.356 2.367 2.217
5001.599 1.687 1.643 1.493
2500.933 0.997 0.965 0.815
1250.541 0.568 0.555 0.405
62.50.355 0.326 0.341 0.191
31.20.222 0.236 0.229 0.079
7.80.154 0.167 0.161 0.011
00.144 0.156 0.150  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CHRDL1 ELISA Kit was designed for the quantitative measurement of Mouse CHRDL1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 1.95 pg/mL .

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Target Background

Function
(From Uniprot)
Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development.
Gene References into Functions
  1. CHRDL-1 is a new regulatory target of miR-134 during cortical neuronal differentiation; this is a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
  2. Reperfusion injury of proximal kidney tubules causes a reduction of Chrdl1 expression. PMID: 19357253
Subcellular Location
Secreted.
Tissue Specificity
Expressed in heart, brain, lung, liver, kidney and testis.
Database Links
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