Mouse Complement 4,C4 ELISA Kit

Instructions
Code CSB-E08707m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 4B (Chido blood group)
Alternative Names C4b ELISA kit; C4Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA kit
Abbreviation C4B
Uniprot No. P01029
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %91
Range %86-95
1:200Average %102
Range %97-107
1:400Average %91
Range %85-97
1:800Average %97
Range %91-103
Recovery
The recovery of mouse C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mOD1OD2AverageCorrected
102.605 2.648 2.627 2.463
52.312 2.335 2.324 2.160
2.51.857 1.867 1.862 1.698
1.251.136 1.103 1.120 0.956
0.6250.798 0.785 0.792 0.628
0.3120.435 0.412 0.424 0.260
0.1560.316 0.304 0.310 0.146
00.163 0.165 0.164
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity).
Gene References into Functions
  1. These data show that Borrelia burgdorferi OspC inhibits the classical and lectin complement pathways and competes with complement protein C2 for C4b binding. PMID: 28873507
  2. C4 mediated synapse elimination during postnatal development PMID: 26814963
  3. role in suppressing autoantibody production elicited by S. pneumoniae antigens PMID: 25339671
  4. a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model PMID: 23259761
  5. C4 modulates interstitial inflammation in experimental glomerulonephritis. PMID: 11726230
  6. Complement C4 provides an important protective role against the development of systemic lupus erythematosus. PMID: 11801636
  7. identified the murine follicular dendritic cell marker, FDC-M2, as complement c4. FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease PMID: 12115608
  8. C4 normally helps prevent early stages of autoimmune disease and that C4 deficiency predisposes to abnormal regulation of autoreactive B cells. PMID: 12207352
  9. Macrophages derived from bone marrow produce sufficient C4 protein to restore the humoral response to NP-KLH hapten in C4-deficient animals when administered along with thymus-dependent antigen. PMID: 12421924
  10. effects of complement depletion and deficiencies of C4, C5, and complement receptors 1 and 2 on mouse survival after intravenous exposure to S aureus PMID: 15192652
  11. We found that deletion of the C4 gene does not significantly change either the time of onset or the severity and tempo of myelin oligodendrocyte-induced EAE compared with controls with a fully intact complement system. PMID: 15390104
  12. In comparison with C57BL/6 mice, MRL/1pr mice had depressed C4 levels as early as 3 weeks of age. PMID: 17971229
  13. The increased mortality of C4(-/-) or Factor B(-/-) mice indicates that these two pathways of complement activation are required for survival in mousepox. PMID: 19112490
  14. Increased transcription of the complement C4 gene in the Rag1(-/-) intestine. PMID: 19200601

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Subcellular Location Secreted, Cell junction, synapse, Cell projection, axon, Cell projection, dendrite
Database Links

KEGG: mmu:12268

STRING: 10090.ENSMUSP00000069418

UniGene: Mm.439678

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