Mouse Complement 4,C4 ELISA Kit

Product Details

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Target Background

Product Details

Target Name

complement component 4B (Chido blood group)

Alternative Names

C4b ELISA kit; C4Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA kit

Abbreviation

C4B

Uniprot No.

P01029

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 μg/mL-10 μg/mL

Sensitivity

0.039 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

Recovery

The recovery of mouse C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/m	OD1	OD2	Average	Corrected
10	2.605	2.648	2.627	2.463
5	2.312	2.335	2.324	2.160
2.5	1.857	1.867	1.862	1.698
1.25	1.136	1.103	1.120	0.956
0.625	0.798	0.785	0.792	0.628
0.312	0.435	0.412	0.424	0.260
0.156	0.316	0.304	0.310	0.146
0	0.163	0.165	0.164

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse C4B ELISA Kit was designed for the quantitative measurement of Mouse C4B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

Target Background

Function
(From Uniprot)

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.

Gene References into Functions

These data show that Borrelia burgdorferi OspC inhibits the classical and lectin complement pathways and competes with complement protein C2 for C4b binding. PMID: 28873507
C4 mediated synapse elimination during postnatal development PMID: 26814963
role in suppressing autoantibody production elicited by S. pneumoniae antigens PMID: 25339671
a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model PMID: 23259761
C4 modulates interstitial inflammation in experimental glomerulonephritis. PMID: 11726230
Complement C4 provides an important protective role against the development of systemic lupus erythematosus. PMID: 11801636
identified the murine follicular dendritic cell marker, FDC-M2, as complement c4. FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease PMID: 12115608
C4 normally helps prevent early stages of autoimmune disease and that C4 deficiency predisposes to abnormal regulation of autoreactive B cells. PMID: 12207352
Macrophages derived from bone marrow produce sufficient C4 protein to restore the humoral response to NP-KLH hapten in C4-deficient animals when administered along with thymus-dependent antigen. PMID: 12421924
effects of complement depletion and deficiencies of C4, C5, and complement receptors 1 and 2 on mouse survival after intravenous exposure to S aureus PMID: 15192652
We found that deletion of the C4 gene does not significantly change either the time of onset or the severity and tempo of myelin oligodendrocyte-induced EAE compared with controls with a fully intact complement system. PMID: 15390104
In comparison with C57BL/6 mice, MRL/1pr mice had depressed C4 levels as early as 3 weeks of age. PMID: 17971229
The increased mortality of C4(-/-) or Factor B(-/-) mice indicates that these two pathways of complement activation are required for survival in mousepox. PMID: 19112490
Increased transcription of the complement C4 gene in the Rag1(-/-) intestine. PMID: 19200601

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Subcellular Location

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.

Database Links

KEGG: mmu:12268

STRING: 10090.ENSMUSP00000069418

UniGene: Mm.439678

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Code	CSB-E08707m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.