Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

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Description

This Mouse CRHBP ELISA Kit was designed for the quantitative measurement of Mouse CRHBP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name

corticotropin releasing hormone binding protein

Alternative Names

CrhbpCorticotropin-releasing factor-binding protein ELISA kit; CRF-BP ELISA kit; CRF-binding protein ELISA kit; Corticotropin-releasing hormone-binding protein ELISA kit; CRH-BP ELISA kit

Abbreviation

CRHBP

Uniprot No.

Q60571

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRHBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-97
1:2	Average %	96
	Range %	91-103
1:4	Average %	97
	Range %	94-100
1:8	Average %	93
	Range %	88-98

Recovery

The recovery of mouse CRHBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-100
EDTA plasma (n=4)	91	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.582	2.776	2.679	2.501
500	2.193	2.223	2.208	2.030
250	1.446	1.465	1.456	1.278
125	0.821	0.865	0.843	0.665
62.5	0.565	0.612	0.589	0.411
31.25	0.319	0.295	0.307	0.129
15.6	0.256	0.246	0.251	0.073
0	0.178	0.177	0.178

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
Gene References into Functions	Repeated cycles of binge drinking alter CRF-BP mRNA expression in the medial prefrontal cortex. PMID: 27374820 Ventral tegmental area CRH-BP is involved in the initial stages of escalated ethanol drinking. PMID: 26247973 These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice. PMID: 25731977 estrogen-regulated expression of corticotropin-releasing hormone-binding protein in lactotropes in female mice suggests that the pituitary is an important site for interactions between the hypothalamic-pituitary-adrenal axis and other endocrine systems PMID: 12446601 Evidence is presented suggesting that the CRH-BP may be required for corticotropin-releasing factor-induced potentiation of NMDA receptors in dopaminergic neurons of the ventral tegmental area. PMID: 12895416 GnRH induces a 3.7-fold increase in CRH-BP mRNA levels via multiple intracellular signaling pathways and a multipartite GnRH response element. PMID: 15976007 analysis of regulation and function of CRH-BP [review] PMID: 16368564 Deletion of corticotropin-releasing factor binding protein selectively impairs maternal, but not intermale aggression. PMID: 18929624 Show More Hide All
Subcellular Location	Secreted.
Protein Families	CRF-binding protein family
Database Links	KEGG: mmu:12919 STRING: 10090.ENSMUSP00000042578 UniGene: Mm.316614

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Check Code：

Code	CSB-EL005964MO
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.