Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

Code CSB-EL005964MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
corticotropin releasing hormone binding protein
Alternative Names
CrhbpCorticotropin-releasing factor-binding protein ELISA kit; CRF-BP ELISA kit; CRF-binding protein ELISA kit; Corticotropin-releasing hormone-binding protein ELISA kit; CRH-BP ELISA kit
Abbreviation
CRHBP
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRHBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %89-97
1:2Average %96
Range %91-103
1:4Average %97
Range %94-100
1:8Average %93
Range %88-98
Recovery
The recovery of mouse CRHBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-100
EDTA plasma (n=4)9184-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.582 2.776 2.679 2.501
5002.193 2.223 2.208 2.030
2501.446 1.465 1.456 1.278
1250.821 0.865 0.843 0.665
62.50.565 0.612 0.589 0.411
31.250.319 0.295 0.307 0.129
15.60.256 0.246 0.251 0.073
00.178 0.177 0.178  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CRHBP ELISA Kit was designed for the quantitative measurement of Mouse CRHBP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
Gene References into Functions
  1. Repeated cycles of binge drinking alter CRF-BP mRNA expression in the medial prefrontal cortex. PMID: 27374820
  2. Ventral tegmental area CRH-BP is involved in the initial stages of escalated ethanol drinking. PMID: 26247973
  3. These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice. PMID: 25731977
  4. estrogen-regulated expression of corticotropin-releasing hormone-binding protein in lactotropes in female mice suggests that the pituitary is an important site for interactions between the hypothalamic-pituitary-adrenal axis and other endocrine systems PMID: 12446601
  5. Evidence is presented suggesting that the CRH-BP may be required for corticotropin-releasing factor-induced potentiation of NMDA receptors in dopaminergic neurons of the ventral tegmental area. PMID: 12895416
  6. GnRH induces a 3.7-fold increase in CRH-BP mRNA levels via multiple intracellular signaling pathways and a multipartite GnRH response element. PMID: 15976007
  7. analysis of regulation and function of CRH-BP [review] PMID: 16368564
  8. Deletion of corticotropin-releasing factor binding protein selectively impairs maternal, but not intermale aggression. PMID: 18929624

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Subcellular Location
Secreted.
Protein Families
CRF-binding protein family
Database Links
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