Mouse Cytochrome-C,Cyt-C ELISA Kit

Code CSB-E08532m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name cytochrome c, somatic
Alternative Names CYC, HCS, THC4, OTTHUMP00000202043|OTTHUMP00000202044|cytochrome c
Abbreviation CYCS
Uniprot No. P62897
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 34.3 pg/mL-2200 pg/mL
Sensitivity 8.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-97
1:2 Average % 84
Range % 80-88
1:4 Average % 90
Range % 85-94
1:8 Average % 97
Range % 92-101
The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 95-105
EDTA plasma (n=4) 87 83-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2200 2.352 2.512 2.432 2.240
1100 1.615 1.578 1.597 1.405
550 1.073 1.023 1.048 0.856
275 0.639 0.681 0.660 0.468
137.5 0.461 0.489 0.475 0.283
68.75 0.387 0.374 0.381 0.189
34.3 0.318 0.305 0.312 0.120
0 0.194 0.189 0.192  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular Location Mitochondrion intermembrane space
Protein Families Cytochrome c family
Tissue Specificity Found in embryos and in adult liver and heart.
Database Links

KEGG: mmu:13063

STRING: 10090.ENSMUSP00000124523

UniGene: Mm.35389

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