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Mouse Cytochrome-C,Cyt-C ELISA Kit

Citations (5) Protocol MSDS
Code CSB-E08532m
Size 96T,5×96T,10×96T
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Product Details

Target Name
cytochrome c, somatic
Alternative Names
Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit
Abbreviation
CYCS
Uniprot No.
P62897
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
34.3 pg/mL-2200 pg/mL
Sensitivity
8.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 92
Range % 88-97
1:2 Average % 84
Range % 80-88
1:4 Average % 90
Range % 85-94
1:8 Average % 97
Range % 92-101
Recovery
The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 95-105
EDTA plasma (n=4) 87 83-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2200 2.352 2.512 2.432 2.240
1100 1.615 1.578 1.597 1.405
550 1.073 1.023 1.048 0.856
275 0.639 0.681 0.660 0.468
137.5 0.461 0.489 0.475 0.283
68.75 0.387 0.374 0.381 0.189
34.3 0.318 0.305 0.312 0.120
0 0.194 0.189 0.192 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CYCS ELISA Kit was designed for the quantitative measurement of Mouse CYCS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 34.3 pg/mL-2200 pg/mL and the sensitivity is 8.5 pg/mL.

Citations

Related Products

Cycs Proteins

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Target Background

Function
(From Uniprot)
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Gene References into Functions
  1. the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning PMID: 25716737
  2. Data suggest that peroxidase activation of cytochrome c may induce apoptosis and contribute to anti-cancer properties of alpha-tocopherol succinate. PMID: 25278024
  3. Data indicate that mitochondrial alpha 7 nicotinic acetylcholine receptors (alpha7 nAChRs) regulate cytochrome c (cyt c) release by engaging intramitochondrial protein kinases. PMID: 24412630
  4. proposed that mutation of residue 41, and interaction with cardiolipin, increase peroxidase activity by altering the 40-57 Omega loop and its hydrogen bond network with the propionate of haem ring A; these changes enhance access of hydrogen peroxide and substrate to the haem PMID: 24329121
  5. Data indicate that Y48H missense mutation of cytochrome c (CYCS) gene is associated with respiratory reduction and increased apoptosis in fibroblast. PMID: 24326104
  6. Translocation of a Bak C-terminus mutant from cytosol to mitochondria to mediate cytochrome C release: implications for Bak and Bax apoptotic function. PMID: 22442658
  7. mitochondrial import and direct electron transfer from cytochrome c to Rac1 modulates mitochondrial H(2)O(2) production in alveolar macrophages pulmonary fibrosis. PMID: 22157762
  8. The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c. PMID: 11864976
  9. results suggest that activation of mitochondrial ATP-dependent potassium channels protects neurons against apoptosis by preventing cytochrome c release from the mitochondria PMID: 11919514
  10. Both neurons and cancer cells strictly inhibit cytochrome c-mediated apoptosis by a mechanism dependent on glucose metabolism. PMID: 19029908

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Subcellular Location
Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
Protein Families
Cytochrome c family
Tissue Specificity
Found in embryos and in adult liver and heart.
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Related pathways
p53 signaling pathway Apoptosis
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