Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

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Target Background

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Product Details

Target Name

SMAD specific E3 ubiquitin protein ligase 1

Alternative Names

Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit

Abbreviation

SMURF1

Uniprot No.

Q9CUN6

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	92
	Range %	88-95
1:4	Average %	86
	Range %	80-91
1:8	Average %	89
	Range %	83-98

Recovery

The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	101	94-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.804	2.600	2.702	2.535
1000	2.403	2.371	2.387	2.220
500	1.747	1.850	1.799	1.632
250	0.972	0.975	0.974	0.807
125	0.594	0.581	0.588	0.421
62.5	0.370	0.347	0.359	0.192
31.25	0.256	0.245	0.251	0.084
0	0.170	0.163	0.167

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
Gene References into Functions	Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis PMID: 30143635 Smurf1 expression in osteoblast differentiation is regulated by CRTC2. PMID: 30449452 Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604 Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690 TGF-beta inhibited osteoblastic differentiation by inducing the MAPK-ERK pathway which upregulated the expression of ubiquitin ligase SMURF1 and resulted in reduced presence of osteogenic proteins. PMID: 28322449 demonstration that Smurf1 acts as a brake for integrin activation by controlling Kindlin-2 protein levels, a new mechanism that permits precise modulation of integrin-mediated cellular functions PMID: 28408404 miR-140-5p and SMURF1 are key regulators of disease pathology in pulmonary arterial hypertension PMID: 27214554 Smurf1 is required for selective autophagy of Mycobacterium tuberculosis and host defense against tuberculosis infection. PMID: 28017659 Smurf1 regulates glucose metabolism and inhibits osteoblast differentiation. Smurf1 function requires the presence of serine 148 (S148) in Smurf1. PMID: 27052174 Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation. PMID: 25901863 Smurf-mediated endocytosis of Patched1 is required in sonic hedgehog signal reception PMID: 24925320 Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199 Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799 These results suggest that the JNK/AP-1 and ERK/Runx2 signaling pathways mediate TNF-alpha-dependent Smurf1 transcription. PMID: 23042144 Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288 studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476 ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619 Smurf1 negatively regulates mesenchymal stem cell proliferation and differentiation by controlling JunB turnover through an ubiquitin-proteasome pathway. PMID: 20200942 induction could trigger downregulation of bone morphogenetic protein signaling and cause vascular cell proliferation and remodeling in pulmonary arterial hypertension pulmonary arterial hypertension PMID: 20558834 Smurf1 appears to be an important regulatory factor in osteoblast differentiation and a potential molecular target for identification of bone anabolic agents PMID: 12738770 Smurf1 is required for the myogenic differentiation of C2C12 cells and plays an important regulatory role in the BMP-2-mediated osteoblast conversion PMID: 12871975 Smurf1 plays a specific role in osteoblast differentiation and bone formation in vivo PMID: 14701828 overexpression of Smurf1 specifically promotes Smad1 and Smad5 ubiquitination and degradation in embryonic lung epithelium PMID: 14711801 Mating Smad6 and Smurf1 transgenic mice produced double-transgenic pups with more delayed endochondral ossification than Smad6 transgenic mice, suggesting that Smurf1 supports Smad6 function in vivo. PMID: 15123739 Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. PMID: 15820682 in chronic inflammatory disorders where TNF is increased, TNF induces the expression of ubiquitin ligase Smurf1 and promotes ubiquitination and proteasomal degradation of Smad1 and Runx2, leading to systemic bone loss PMID: 18567580 the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. PMID: 18641638 Mice mutant for Smurf1 and Smurf2 display planar cell polarity defects in the cochlea and convergence and extension movements defects that include a failure to close the neural tube. PMID: 19379695 Initial identification of Smurf1 as an ubiquitin ligase that inhibits BMP signaling by targeting Smad1/5 for proteasomal destruction in cultured cells and Xenopus embryos. PMID: 10458166 Show More Hide All
Subcellular Location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Database Links	KEGG: mmu:75788 STRING: 10090.ENSMUSP00000082827 UniGene: Mm.27735

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Check Code：

Code	CSB-EL021860MO
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.