Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

Code CSB-EL021860MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SMAD specific E3 ubiquitin protein ligase 1
Alternative Names Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit
Abbreviation SMURF1
Uniprot No. Q9CUN6
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %92-104
1:2Average %92
Range %88-95
1:4Average %86
Range %80-91
1:8Average %89
Range %83-98
Recovery
The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-96
EDTA plasma (n=4)10194-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.804 2.600 2.702 2.535
10002.403 2.371 2.387 2.220
5001.747 1.850 1.799 1.632
2500.972 0.975 0.974 0.807
1250.594 0.581 0.588 0.421
62.50.370 0.347 0.359 0.192
31.250.256 0.245 0.251 0.084
00.170 0.163 0.167  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
Gene References into Functions
  1. Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis PMID: 30143635
  2. Smurf1 expression in osteoblast differentiation is regulated by CRTC2. PMID: 30449452
  3. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
  4. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
  5. TGF-beta inhibited osteoblastic differentiation by inducing the MAPK-ERK pathway which upregulated the expression of ubiquitin ligase SMURF1 and resulted in reduced presence of osteogenic proteins. PMID: 28322449
  6. demonstration that Smurf1 acts as a brake for integrin activation by controlling Kindlin-2 protein levels, a new mechanism that permits precise modulation of integrin-mediated cellular functions PMID: 28408404
  7. miR-140-5p and SMURF1 are key regulators of disease pathology in pulmonary arterial hypertension PMID: 27214554
  8. Smurf1 is required for selective autophagy of Mycobacterium tuberculosis and host defense against tuberculosis infection. PMID: 28017659
  9. Smurf1 regulates glucose metabolism and inhibits osteoblast differentiation. Smurf1 function requires the presence of serine 148 (S148) in Smurf1. PMID: 27052174
  10. Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation. PMID: 25901863
  11. Smurf-mediated endocytosis of Patched1 is required in sonic hedgehog signal reception PMID: 24925320
  12. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
  13. Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799
  14. These results suggest that the JNK/AP-1 and ERK/Runx2 signaling pathways mediate TNF-alpha-dependent Smurf1 transcription. PMID: 23042144
  15. Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
  16. studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
  17. ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
  18. Smurf1 negatively regulates mesenchymal stem cell proliferation and differentiation by controlling JunB turnover through an ubiquitin-proteasome pathway. PMID: 20200942
  19. induction could trigger downregulation of bone morphogenetic protein signaling and cause vascular cell proliferation and remodeling in pulmonary arterial hypertension pulmonary arterial hypertension PMID: 20558834
  20. Smurf1 appears to be an important regulatory factor in osteoblast differentiation and a potential molecular target for identification of bone anabolic agents PMID: 12738770
  21. Smurf1 is required for the myogenic differentiation of C2C12 cells and plays an important regulatory role in the BMP-2-mediated osteoblast conversion PMID: 12871975
  22. Smurf1 plays a specific role in osteoblast differentiation and bone formation in vivo PMID: 14701828
  23. overexpression of Smurf1 specifically promotes Smad1 and Smad5 ubiquitination and degradation in embryonic lung epithelium PMID: 14711801
  24. Mating Smad6 and Smurf1 transgenic mice produced double-transgenic pups with more delayed endochondral ossification than Smad6 transgenic mice, suggesting that Smurf1 supports Smad6 function in vivo. PMID: 15123739
  25. Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. PMID: 15820682
  26. in chronic inflammatory disorders where TNF is increased, TNF induces the expression of ubiquitin ligase Smurf1 and promotes ubiquitination and proteasomal degradation of Smad1 and Runx2, leading to systemic bone loss PMID: 18567580
  27. the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. PMID: 18641638
  28. Mice mutant for Smurf1 and Smurf2 display planar cell polarity defects in the cochlea and convergence and extension movements defects that include a failure to close the neural tube. PMID: 19379695
  29. Initial identification of Smurf1 as an ubiquitin ligase that inhibits BMP signaling by targeting Smad1/5 for proteasomal destruction in cultured cells and Xenopus embryos. PMID: 10458166

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Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Database Links

KEGG: mmu:75788

STRING: 10090.ENSMUSP00000082827

UniGene: Mm.27735

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