SMURF1, Smad-specific E3 ubiquitin protein ligase 1, is also known as hSMURF1, KIAA1625. SMURF1 is a HECT-type E3 ubiquitin ligase, which is mainly expressed in human cells, and especially highly expressed in ectodermal tissues during embryonic development.
Its mechanism of action is through interaction with Smad effectors in the BMP (bone morphogenetic protein) pathway, leading to ubiquitination and degradation of Smad proteins, which plays a key role in the regulation of cell motility, cell signaling, and cell polarity.
SMURF1 is important in the processes of cellular differentiation, tissue formation, and tumorigenesis. SMURF1 negatively regulates in vivo osteoblast differentiation and bone formation, and has been associated with a variety of diseases, including neural tube closure defects, Brachydactyly Type A2, and Fanconi anemia.
Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial (CSB-EP875720HU1a0)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
SMURF1 Antibody (CSB-PA875720LA01HU)
Validated Data
Western Blot
Positive WB detected in: U251 whole cell lysate, A549 whole cell lysate
All lanes: SMURF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 84 KDa
Observed band size: 87 KDa
Exposure time:5min
Immunofluorescence staining of A549 cells with CSB-PA875720LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following SMURF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
SMURF1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA021860GA01HU | SMURF1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA026284 | SMURF1 Antibody | Human,Mouse | ELISA,IHC |
CSB-PA875720LA01HU | SMURF1 Antibody | Human | ELISA, WB, IF |
CSB-PA875720LB01HU | SMURF1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA875720LC01HU | SMURF1 Antibody, FITC conjugated | Human | |
CSB-PA875720LD01HU | SMURF1 Antibody, Biotin conjugated | Human | ELISA |
CSB-MA875720A0m | SMURF1 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
SMURF1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP875137MO CSB-EP875137MO CSB-BP875137MO CSB-MP875137MO CSB-EP875137MO-B |
Recombinant Mouse E3 ubiquitin-protein ligase SMURF1 (Smurf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SMURF1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP875720HU CSB-EP875720HU CSB-BP875720HU CSB-MP875720HU CSB-EP875720HU-B |
Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP875720HU1 | Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial | E.coli |
CSB-EP875720HU1a0 | Recombinant Human E3 ubiquitin-protein ligase SMURF1 (SMURF1), partial | E.coli |
SMURF1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP886105XBE CSB-EP886105XBE CSB-BP886105XBE CSB-MP886105XBE CSB-EP886105XBE-B |
Recombinant Xenopus laevis E3 ubiquitin-protein ligase SMURF1 (smurf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SMURF1 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL021860MO | Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 7.8 pg/mL |