Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1(ENPP1) ELISA kit

Code CSB-EL007679MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
ectonucleotide pyrophosphatase/phosphodiesterase 1
Alternative Names
Enpp1 ELISA kit; Npps ELISA kit; Pc1 ELISA kit; Pdnp1Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; E-NPP 1 ELISA kit; Lymphocyte antigen 41 ELISA kit; Ly-41 ELISA kit; Phosphodiesterase I/nucleotide pyrophosphatase 1 ELISA kit; Plasma-cell membrane glycoprotein PC-1) [Cleaved into: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA kit; secreted form] [Includes: Alkaline phosphodiesterase I ELISA kit; EC 3.1.4.1); Nucleotide pyrophosphatase ELISA kit; NPPase ELISA kit; EC 3.6.1.9 ELISA kit; Nucleotide diphosphatase)] ELISA kit
Abbreviation
ENPP1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 93
Range % 89-97
1:10 Average % 90
Range % 82-99
1:20 Average % 95
Range % 87-101
1:40 Average % 95
Range % 85-104
Recovery
The recovery of mouse ENPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 88-99
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.591 2.575 2.583 2.489
10 1.465 1.492 1.479 1.385
5 0.786 0.802 0.794 0.700
2.5 0.424 0.438 0.431 0.337
1.25 0.272 0.274 0.273 0.179
0.625 0.168 0.172 0.170 0.076
0.312 0.127 0.129 0.128 0.034
0 0.092 0.095 0.094  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse ENPP1 ELISA Kit was designed for the quantitative measurement of Mouse ENPP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect. Appears to modulate insulin sensitivity. Also involved in melanogenesis. Also able to hydrolyze 2'-3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2'-3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2'-3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2'-3'-cGAMP linkage isomer 3'-3'-cGAMP.
Gene References into Functions
  1. Runt-related transcription factor 2 (Runx2) was confirmed to have synergistic effects with Osx in activating ENPP1 promoter. PMID: 30047979
  2. Report the temporal ectopic mineralization phenotype of connective tissues in Enpp1asj-2J mouse model for generalized arterial calcification of infancy. PMID: 27863377
  3. Evidence of a causative link between ENPP1 and alterations in insulin signaling, glucose uptake, and lipid metabolism in subcutaneous abdominal Adipose tissue of gestational diabetes, which may mediate insulin resistance and hyperglycemia in Gestational diabetes. PMID: 28080219
  4. Results show that urine pyrophosphate (PPI) levels are increased in Npt2a-/- mice when compared to WT, possibly to protect from renal mineralization in the setting of hyperphosphaturia. However, the presence of two hypomorphic Enpp1asj/asj alleles decreases urine PPi and worsens renal calcium phosphate deposit formation in Npt2a-/- mice. PMID: 28704395
  5. Enpp1asj mutant mouse provides a new animal model for studying tympanosclerotic otitis and otitis media with effusion PMID: 27959908
  6. identified ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) as the first known mammalian enzyme lacking a NUDIX domain to generate pR from ADP-ribose on modified proteins in vitro PMID: 27406238
  7. Vitamin D3 regulates Enpp1 expression, which presumably, in the context of adequate tissue non-specific alkaline phosphatase activity, provides phosphate to stimulate mineralisation. PMID: 26054750
  8. ENPP1-Fc fusion protein prevents the mortality, vascular calcifications and sequela of disease in mouse models of generalized arterial calcification of infancy. PMID: 26624227
  9. Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
  10. Increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions. PMID: 26033523
  11. NPP1 has a role in obesity and diabetes in a mouse model PMID: 25368121
  12. we have characterized the phenotypic and histopathologic features of this spontaneous mutant mouse, designated as asj-2J, and we have identified a large deletion/insertion mutation in the Enpp1 gene PMID: 25479107
  13. these data highlight the key role of NPP1 in regulating calcification of both soft and skeletal tissues. PMID: 25260930
  14. ENPP1 2'3'-cGAMP-hydrolyzing activity is repsonsible for tumor progression in humans and mice. PMID: 25344812
  15. Both mono-allelic and bi-allelic ENPP1 deficiency promote dysregulated VSMC function, with robust C/EBP homologous protein expression and enhanced neointimal hyperplasia after injury in vivo, but marked post-injury calcification limited to Enpp1-/- mice. PMID: 24530784
  16. Its mutaion causes osteochondral diseases and malnutrition. PMID: 24770645
  17. Studied the role of genetic modulation and the role of diet in nephrocalcinosis using two established mouse models of ectopic mineralization, Abcc6(tm1Jfk) and Enpp1(asj) mice. PMID: 24732453
  18. The mutant ENPP1 protein is largely absent in the liver of 'ages with stiffened joints' mice, and the lack of enzymatic activity results in reduced inorganic pyrophosphate (PPi) levels in the plasma, accompanied by extensive mineralization. PMID: 23798568
  19. these data suggest a potential role for Enpp1 in the development of breast cancer bone metastasis. PMID: 23861746
  20. Expression of plasma cell alloantigen 1 defines layered development of B-1a B-cell subsets with distinct innate-like functions. PMID: 23169635
  21. crystal of Enpp1 belonged to space group P3(1), with unit-cell parameters a = b = 105.3, c = 173.7 A. PMID: 22750863
  22. Decreased levels of nucleotide pyro- phosphatase phosphodiesterase 1 trigger osteoarthritic changes in mice. PMID: 22510396
  23. NPP1 is essential for normal bone development and control of physiological bone mineralization PMID: 22359666
  24. Npp1 promotes atherosclerosis, potentially mediated by Opn expression in ApoE knockout mice PMID: 21477221
  25. ENPP1 plays multiple and distinct roles in the development of mineralized tissues and that the influence of ENPP1 on osteoblast differentiation and gene expression may include a mechanism that is independent of its catalytic activity. PMID: 21930712
  26. RAGE promotes ectopic chondrogenic differentiation and arterial calcification in Enpp1-/- mice. PMID: 21099228
  27. NPP1 does not have a major role in PP(i) generation from ATP at the level of MVs, in contrast to its accepted role on the surface of the osteoblasts and chondrocytes, but rather acts as a phosphatase in the absence of TNAP. PMID: 19874193
  28. Data show that reconstitution of TNAP and NPP1 into proteoliposome membranes generates a phospholipid microenvironment that allows the kinetic study of phosphosubstrate catabolism in a manner that recapitulates the native MV microenvironment. PMID: 20048161
  29. ENPP1 expression was found down-regulated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation PMID: 17849011
  30. correction of bone mineralization abnormalities in knockout mice null for both the TNAP (Akp2) and PC-1 (Enpp1) genes. PMID: 12082181
  31. functional characterization of the non-catalytic ectodomains of this enzyme PMID: 12533192
  32. Linked regulatory effects on extracellular PP(i) and OPN expression mediate the ability of PC-1 and ANK to regulate calcification. PMID: 12817751
  33. Specific targeting motif in the NPP1 cytosolic tail that delivers PP(i)-generating NPP activity to osteoblast matrix vesicles for control of calcification. PMID: 15075217
  34. NPP1 and PP(i) deficiencies modulate phenotype plasticity in artery smooth muscle cells and chondrogenesis in mesenchymal precursors PMID: 15625282
  35. Lubrol resistance is an intrinsic property of NPP3 but not NPP1, which is acquired early during the biosynthetic process irrespective of its final destination, and depends on positively charged residues in its cytoplasmic tail. PMID: 17311850
  36. expression of PC-1 downstream of fibroblast growth factor signaling is an integral aspect of osteoblastic function and pyrophosphate elaboration may play a significant role in the pathology of craniosynostosis PMID: 17552941
  37. These results indicate that the mechanism of PC-1 induction by FGF2 in preosteoblastic cells is distinct from the mechanism of induction that occurs with osteoblast differentiation. PMID: 18698132
  38. FGF2 is a specific inducer of PC-1 in pre-osteoblast cells and that FGF2 induces PC-1 expression through a mechanism involving Runx2. PMID: 19049325
  39. results support a role for the Enpp1 gene in the pathogenesis associated with mineralization of articular cartilage and vascular calcification PMID: 19419305
  40. Suppression of PC-1 expression improves insulin sensitivity in vitro and in an animal model of diabetes PMID: 19577557

Show More

Hide All

Involvement in disease
Defects in Enpp1 are the cause of the tiptoe walking (ttw) phenotype. Ttw mice exhibit ossification of the spinal ligaments.
Subcellular Location
[Ectonucleotide pyrophosphatase/phosphodiesterase family member 1]: Cell membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein.; [Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form]: Secreted.
Protein Families
Nucleotide pyrophosphatase/phosphodiesterase family
Tissue Specificity
Selectively expressed on the surface of antibody-secreting cells. Expressed in osteocytes and osteoclasts.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X