Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Enpp1

Uniprot No.

P06802

Research Area

Metabolism

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

635-906aa

Target Protein Sequence

HMTVPYGRPRILLKQHHVCLLQQQQFLTGYSLDLLMPLWASYTFLRNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.8 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To produce a recombinant mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (Enpp1) protein, the gene fragment encoding the amino acids 635-906 of the mouse Enpp1 along with an N-terminal 6xHis-tag is inserted into a suitable expression vector. The constructed vector is then transformed into E. coli cells to enable protein expression. The transformed cells are selected, and a large-scale culture is established to promote efficient production of the recombinant Enpp1 protein. The N-terminal 6xHis-tag facilitates easy purification and detection of the recombinant Enpp1 protein. The purity of the recombinant mouse Enpp1 protein is confirmed to be up to 90% through SDS-PAGE analysis. On the gel, the recombinant Enpp1 protein appears as a distinct band with an approximate molecular weight of 38 kDa.

Target Background

Function

Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect. Appears to modulate insulin sensitivity. Also involved in melanogenesis. Also able to hydrolyze 2'-3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2'-3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2'-3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2'-3'-cGAMP linkage isomer 3'-3'-cGAMP.

Gene References into Functions

Runt-related transcription factor 2 (Runx2) was confirmed to have synergistic effects with Osx in activating ENPP1 promoter. PMID: 30047979
Report the temporal ectopic mineralization phenotype of connective tissues in Enpp1asj-2J mouse model for generalized arterial calcification of infancy. PMID: 27863377
Evidence of a causative link between ENPP1 and alterations in insulin signaling, glucose uptake, and lipid metabolism in subcutaneous abdominal Adipose tissue of gestational diabetes, which may mediate insulin resistance and hyperglycemia in Gestational diabetes. PMID: 28080219
Results show that urine pyrophosphate (PPI) levels are increased in Npt2a-/- mice when compared to WT, possibly to protect from renal mineralization in the setting of hyperphosphaturia. However, the presence of two hypomorphic Enpp1asj/asj alleles decreases urine PPi and worsens renal calcium phosphate deposit formation in Npt2a-/- mice. PMID: 28704395
Enpp1asj mutant mouse provides a new animal model for studying tympanosclerotic otitis and otitis media with effusion PMID: 27959908
identified ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) as the first known mammalian enzyme lacking a NUDIX domain to generate pR from ADP-ribose on modified proteins in vitro PMID: 27406238
Vitamin D3 regulates Enpp1 expression, which presumably, in the context of adequate tissue non-specific alkaline phosphatase activity, provides phosphate to stimulate mineralisation. PMID: 26054750
ENPP1-Fc fusion protein prevents the mortality, vascular calcifications and sequela of disease in mouse models of generalized arterial calcification of infancy. PMID: 26624227
Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
Increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions. PMID: 26033523
NPP1 has a role in obesity and diabetes in a mouse model PMID: 25368121
we have characterized the phenotypic and histopathologic features of this spontaneous mutant mouse, designated as asj-2J, and we have identified a large deletion/insertion mutation in the Enpp1 gene PMID: 25479107
these data highlight the key role of NPP1 in regulating calcification of both soft and skeletal tissues. PMID: 25260930
ENPP1 2'3'-cGAMP-hydrolyzing activity is repsonsible for tumor progression in humans and mice. PMID: 25344812
Both mono-allelic and bi-allelic ENPP1 deficiency promote dysregulated VSMC function, with robust C/EBP homologous protein expression and enhanced neointimal hyperplasia after injury in vivo, but marked post-injury calcification limited to Enpp1-/- mice. PMID: 24530784
Its mutaion causes osteochondral diseases and malnutrition. PMID: 24770645
Studied the role of genetic modulation and the role of diet in nephrocalcinosis using two established mouse models of ectopic mineralization, Abcc6(tm1Jfk) and Enpp1(asj) mice. PMID: 24732453
The mutant ENPP1 protein is largely absent in the liver of 'ages with stiffened joints' mice, and the lack of enzymatic activity results in reduced inorganic pyrophosphate (PPi) levels in the plasma, accompanied by extensive mineralization. PMID: 23798568
these data suggest a potential role for Enpp1 in the development of breast cancer bone metastasis. PMID: 23861746
Expression of plasma cell alloantigen 1 defines layered development of B-1a B-cell subsets with distinct innate-like functions. PMID: 23169635
crystal of Enpp1 belonged to space group P3(1), with unit-cell parameters a = b = 105.3, c = 173.7 A. PMID: 22750863
Decreased levels of nucleotide pyro- phosphatase phosphodiesterase 1 trigger osteoarthritic changes in mice. PMID: 22510396
NPP1 is essential for normal bone development and control of physiological bone mineralization PMID: 22359666
Npp1 promotes atherosclerosis, potentially mediated by Opn expression in ApoE knockout mice PMID: 21477221
ENPP1 plays multiple and distinct roles in the development of mineralized tissues and that the influence of ENPP1 on osteoblast differentiation and gene expression may include a mechanism that is independent of its catalytic activity. PMID: 21930712
RAGE promotes ectopic chondrogenic differentiation and arterial calcification in Enpp1-/- mice. PMID: 21099228
NPP1 does not have a major role in PP(i) generation from ATP at the level of MVs, in contrast to its accepted role on the surface of the osteoblasts and chondrocytes, but rather acts as a phosphatase in the absence of TNAP. PMID: 19874193
Data show that reconstitution of TNAP and NPP1 into proteoliposome membranes generates a phospholipid microenvironment that allows the kinetic study of phosphosubstrate catabolism in a manner that recapitulates the native MV microenvironment. PMID: 20048161
ENPP1 expression was found down-regulated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation PMID: 17849011
correction of bone mineralization abnormalities in knockout mice null for both the TNAP (Akp2) and PC-1 (Enpp1) genes. PMID: 12082181
functional characterization of the non-catalytic ectodomains of this enzyme PMID: 12533192
Linked regulatory effects on extracellular PP(i) and OPN expression mediate the ability of PC-1 and ANK to regulate calcification. PMID: 12817751
Specific targeting motif in the NPP1 cytosolic tail that delivers PP(i)-generating NPP activity to osteoblast matrix vesicles for control of calcification. PMID: 15075217
NPP1 and PP(i) deficiencies modulate phenotype plasticity in artery smooth muscle cells and chondrogenesis in mesenchymal precursors PMID: 15625282
Lubrol resistance is an intrinsic property of NPP3 but not NPP1, which is acquired early during the biosynthetic process irrespective of its final destination, and depends on positively charged residues in its cytoplasmic tail. PMID: 17311850
expression of PC-1 downstream of fibroblast growth factor signaling is an integral aspect of osteoblastic function and pyrophosphate elaboration may play a significant role in the pathology of craniosynostosis PMID: 17552941
These results indicate that the mechanism of PC-1 induction by FGF2 in preosteoblastic cells is distinct from the mechanism of induction that occurs with osteoblast differentiation. PMID: 18698132
FGF2 is a specific inducer of PC-1 in pre-osteoblast cells and that FGF2 induces PC-1 expression through a mechanism involving Runx2. PMID: 19049325
results support a role for the Enpp1 gene in the pathogenesis associated with mineralization of articular cartilage and vascular calcification PMID: 19419305
Suppression of PC-1 expression improves insulin sensitivity in vitro and in an animal model of diabetes PMID: 19577557

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Involvement in disease

Defects in Enpp1 are the cause of the tiptoe walking (ttw) phenotype. Ttw mice exhibit ossification of the spinal ligaments.

Subcellular Location

[Ectonucleotide pyrophosphatase/phosphodiesterase family member 1]: Cell membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein.; [Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form]: Secreted.

Protein Families

Nucleotide pyrophosphatase/phosphodiesterase family

Tissue Specificity

Selectively expressed on the surface of antibody-secreting cells. Expressed in osteocytes and osteoclasts.

Database Links

KEGG: mmu:18605

STRING: 10090.ENSMUSP00000101159

UniGene: Mm.27254

Code	CSB-EP007679MO
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (Enpp1), partial

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