Product Details

Target Name

CD248 molecule, endosialin

Alternative Names

Cd248 ELISA Kit; Tem1 ELISA Kit; Endosialin ELISA Kit; Tumor endothelial marker 1 ELISA Kit; CD antigen CD248 ELISA Kit

Abbreviation

CD248

Uniprot No.

Q91V98

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.81 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-99
1:200	Average %	102
	Range %	96-107
1:400	Average %	93
	Range %	89-98
1:800	Average %	86
	Range %	80-92

Recovery

The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	98	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.643	2.781	2.712	2.625
250	1.765	1.822	1.794	1.707
125	1.121	1.211	1.166	1.079
62.5	0.721	0.710	0.716	0.629
31.25	0.433	0.448	0.441	0.354
15.62	0.276	0.282	0.279	0.192
7.81	0.182	0.188	0.185	0.098
0	0.086	0.088	0.087

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse CD248 ELISA Kit was designed for the quantitative measurement of Mouse CD248 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.81 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target Background

Function
(From Uniprot)

May play a role in angiogenesis or vascular function.

Gene References into Functions

the data demonstrate a critical role for endosialin-expressing primary tumor pericytes in mediating metastatic dissemination and identify endosialin as a promising therapeutic target in breast cancer PMID: 27635044
Our data show that deletion of CD248 reduces susceptibility to liver fibrosis via an effect on PDGF signaling. PMID: 26078290
Endosialin is a potential regulator of phenotypic remodeling of vascular smooth muscle cells contributing to atherosclerosis. PMID: 28126825
suggest that CD248 stromal cells have a pathogenic role in renal fibrosis PMID: 26633297
We conclude that CD248 is required for PDGFRbeta-dependant capillary sprouting but not splitting angiogenesis, and identify a new role for CD248 expressed on pericytes in the early stages of physiological angiogenesis during muscle remodelling. PMID: 25243742
Tem1-TT vaccination elicited CD8+ and/or CD4+ T cell responses against immunodominant TEM1 protein sequences. Prophylactic immunization of animals with Tem1-TT prevented or delayed tumor formation in several tumor models. PMID: 24642465
Expression of CD248 was seen in osteoblasts, but not osteoclasts. CD248(-/-) mouse tibiae had higher bone mass and superior mechanical properties compared to WT mice. Primary osteoblasts from CD248(-/-) mice induced increased bone mineralization PMID: 22674221
The multiple pathways regulated by the cytoplasmic domain of CD248 highlight its potential as a therapeutic target to treat cancer. PMID: 21549007
our observations suggest that Tem1 is expressed throughout embryonic and adult development in several types of mesenchymal cells closely related to blood vessels. PMID: 21402174
required for efficient lymph node expansion PMID: 20432232
Tem1 have significant implications for the mechanisms underlying tumor invasiveness and for models that evaluate this process PMID: 16492758
In the embryo when analysed by confocal microscopy endosialin on vessels does not colocalise with endothelial cells expressing CD31, rather, endosialin is restricted to closely associated perivascular cells that also express the pericyte marker NG2. PMID: 16965941
CD248 expression helps define a subset of lymphoid tissue stromal cells which play a role in remodelling during tissue development, infection and repair PMID: 17628549

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in cell lines derived from endothelial cells, embryonic fibroblasts and preadipocytes.

Database Links

KEGG: mmu:70445

STRING: 10090.ENSMUSP00000070847

UniGene: Mm.29597

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Code	CSB-EL004905MO
Size	96T,5×96T,10×96T
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Mouse Endosialin(CD248) ELISA kit

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Target Background