Mouse Endosialin(CD248) ELISA kit

Instructions
Code CSB-EL004905MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD248 molecule, endosialin
Alternative Names Cd248 ELISA Kit; Tem1 ELISA Kit; Endosialin ELISA Kit; Tumor endothelial marker 1 ELISA Kit; CD antigen CD248 ELISA Kit
Abbreviation CD248
Uniprot No. Q91V98
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.81 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 95
Range % 90-99
1:200 Average % 102
Range % 96-107
1:400 Average % 93
Range % 89-98
1:800 Average % 86
Range % 80-92
Recovery
The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 98 94-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.643 2.781 2.712 2.625
250 1.765 1.822 1.794 1.707
125 1.121 1.211 1.166 1.079
62.5 0.721 0.710 0.716 0.629
31.25 0.433 0.448 0.441 0.354
15.62 0.276 0.282 0.279 0.192
7.81 0.182 0.188 0.185 0.098
0 0.086 0.088 0.087  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
May play a role in angiogenesis or vascular function.
Gene References into Functions
  1. the data demonstrate a critical role for endosialin-expressing primary tumor pericytes in mediating metastatic dissemination and identify endosialin as a promising therapeutic target in breast cancer PMID: 27635044
  2. Our data show that deletion of CD248 reduces susceptibility to liver fibrosis via an effect on PDGF signaling. PMID: 26078290
  3. Endosialin is a potential regulator of phenotypic remodeling of vascular smooth muscle cells contributing to atherosclerosis. PMID: 28126825
  4. suggest that CD248 stromal cells have a pathogenic role in renal fibrosis PMID: 26633297
  5. We conclude that CD248 is required for PDGFRbeta-dependant capillary sprouting but not splitting angiogenesis, and identify a new role for CD248 expressed on pericytes in the early stages of physiological angiogenesis during muscle remodelling. PMID: 25243742
  6. Tem1-TT vaccination elicited CD8+ and/or CD4+ T cell responses against immunodominant TEM1 protein sequences. Prophylactic immunization of animals with Tem1-TT prevented or delayed tumor formation in several tumor models. PMID: 24642465
  7. Expression of CD248 was seen in osteoblasts, but not osteoclasts. CD248(-/-) mouse tibiae had higher bone mass and superior mechanical properties compared to WT mice. Primary osteoblasts from CD248(-/-) mice induced increased bone mineralization PMID: 22674221
  8. The multiple pathways regulated by the cytoplasmic domain of CD248 highlight its potential as a therapeutic target to treat cancer. PMID: 21549007
  9. our observations suggest that Tem1 is expressed throughout embryonic and adult development in several types of mesenchymal cells closely related to blood vessels. PMID: 21402174
  10. required for efficient lymph node expansion PMID: 20432232
  11. Tem1 have significant implications for the mechanisms underlying tumor invasiveness and for models that evaluate this process PMID: 16492758
  12. In the embryo when analysed by confocal microscopy endosialin on vessels does not colocalise with endothelial cells expressing CD31, rather, endosialin is restricted to closely associated perivascular cells that also express the pericyte marker NG2. PMID: 16965941
  13. CD248 expression helps define a subset of lymphoid tissue stromal cells which play a role in remodelling during tissue development, infection and repair PMID: 17628549

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in cell lines derived from endothelial cells, embryonic fibroblasts and preadipocytes.
Database Links

KEGG: mmu:70445

STRING: 10090.ENSMUSP00000070847

UniGene: Mm.29597

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