Mouse Eosinophil Peroxidase (EPO) ELISA Kit

Instructions
Code CSB-E15830m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name eosinophil peroxidase
Alternative Names Epx ELISA Kit; Eper ELISA Kit; Eosinophil peroxidase ELISA Kit; EPO ELISA Kit; EC 1.11.1.7) [Cleaved into: Eosinophil peroxidase light chain; Eosinophil peroxidase heavy chain] ELISA Kit
Abbreviation EPX
Uniprot No. P49290
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 84-92  
1:2 Average % 94  
Range % 90-98  
1:4 Average % 99  
Range % 95-104  
1:8 Average % 102  
Range % 98-107  
Recovery
The recovery of mouse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 92-98  
EDTA plasma (n=4) 96 94-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.269 2.298 2.284 2.086  
5 1.674 1.698 1.686 1.488  
2.5 1.081 1.098 1.090 0.892  
1.25 0.729 0.769 0.749 0.551  
0.625 0.437 0.457 0.447 0.249  
0.312 0.388 0.363 0.376 0.178  
0.156 0.283 0.303 0.293 0.095  
0 0.197 0.198 0.198    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils.
Gene References into Functions
  1. The expression of MBP-1 and EPX was also required for induced lung expression of IL-4/IL-13 in each setting and, in turn, the induced pulmonary remodeling events and lung dysfunction. PMID: 28515227
  2. EPO-mediated protein carbamylation is promoted during allergen-induced asthma exacerbation, and can both modulate immune responses and trigger a cascade of many of the inflammatory signals present in asthma. PMID: 27587397
  3. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
  4. HER2 was identified as a novel mediator of eosinophil peroxidase signaling. Eosinophil peroxidase, at noncytotoxic levels, can drive cell-cycle progression and proliferation. PMID: 21454806
  5. Host protection against Brugia pahangi infections was unimpaired in mice deficient in eosinophil peroxidase. PMID: 16299347
  6. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice. PMID: 16926417
  7. post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase PMID: 18694936
  8. The activities of EPO, an eosinophilic secondary granule protein, do not affect the development of allergic pulmonary pathologies in the mouse lung at levels comparable to those observed in humans with asthma. PMID: 11466391

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Subcellular Location Cytoplasmic granule
Protein Families Peroxidase family, XPO subfamily
Database Links

KEGG: mmu:13861

STRING: 10090.ENSMUSP00000050497

UniGene: Mm.1315

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