Mouse Filaggrin(FLG) ELISA kit

Code CSB-EL008712MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
filaggrin
Alternative Names
FlgFilaggrin ELISA Kit; Fragment ELISA Kit
Abbreviation
FLG
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 97  
Range % 92-101  
1:4 Average % 88  
Range % 86-95  
1:8 Average % 90  
Range % 86-95  
Recovery
The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 101 95-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.290 2.433 2.362 2.197  
100 1.503 1.390 1.447 1.282  
50 0.810 0.871 0.841 0.676  
25 0.573 0.610 0.592 0.427  
12.5 0.381 0.366 0.374 0.209  
6.25 0.288 0.274 0.281 0.116  
3.12 0.229 0.239 0.234 0.069  
0 0.163 0.167 0.165    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse FLG ELISA Kit was designed for the quantitative measurement of Mouse FLG protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Target Background

Function
(From Uniprot)
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Subcellular Location
Cytoplasmic granule.
Protein Families
S100-fused protein family
Tissue Specificity
Expressed in the granular layer of the epidermis.
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