Mouse Filaggrin(FLG) ELISA kit

Instructions
Code CSB-EL008712MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name filaggrin
Alternative Names FlgFilaggrin ELISA Kit; Fragment ELISA Kit
Abbreviation FLG
Uniprot No. P11088
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 97  
Range % 92-101  
1:4 Average % 88  
Range % 86-95  
1:8 Average % 90  
Range % 86-95  
Recovery
The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 101 95-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.290 2.433 2.362 2.197  
100 1.503 1.390 1.447 1.282  
50 0.810 0.871 0.841 0.676  
25 0.573 0.610 0.592 0.427  
12.5 0.381 0.366 0.374 0.209  
6.25 0.288 0.274 0.281 0.116  
3.12 0.229 0.239 0.234 0.069  
0 0.163 0.167 0.165    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Subcellular Location Cytoplasmic granule
Protein Families S100-fused protein family
Tissue Specificity Expressed in the granular layer of the epidermis.
Database Links

STRING: 10090.ENSMUSP00000054773

UniGene: Mm.162684

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