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| Code | CSB-E04559m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-105 | |||||
| The recovery of mouse FK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-94 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E04559m is a sandwich ELISA kit developed to measure concentrations of mouse fractalkine (FK) in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FK in samples. The color intensity is measured at 450 nm via a microplate reader.
FK, also called CX3CL1, is a transmembrane chemokine expressed in the central nervous system (CNS) by neuron signals through its unique receptor, CX3CR1 present in microglia. CX3CL1 is involved in the directional movement of chemokine cells, immune response, inflammatory response, tissue repair, as well as other processes. By binding to its receptor CX3CR1, CX3CL1 exerts cell chemotactic effects, induces and recruits NK cells, monocytes, macrophages, mast cells, effector T cells, etc. in order to reach specific inflammatory response sites, and participates in the neutrophil and macrophage killing of pathogens. The CX3CL1/CX3CR1 axis is implicated in the pathogenesis of inflammatory diseases, cardiovascular diseases, and several inflammatory cancer including various B cell malignancies.
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