Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

Instructions
Code CSB-EL012888MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lectin, galactoside-binding, soluble, 3 binding protein
Alternative Names Lgals3bp ELISA Kit; Cycap ELISA Kit; MamaGalectin-3-binding protein ELISA Kit; Cyp-C-associated protein ELISA Kit; CyCAP ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; Protein MAMA ELISA Kit
Abbreviation LGALS3BP
Uniprot No. Q07797
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.39 ng/mL-25 ng/mL
Sensitivity 0.09 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 94
Range % 90-98
1:2000 Average % 102
Range % 96-107
1:4000 Average % 87
Range % 81-92
1:8000 Average % 92
Range % 88-96
Recovery
The recovery of mouse LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 97-107
EDTA plasma (n=4) 98 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
25 2.867 2.723 2.795 2.707
12.5 1.988 1.856 1.922 1.834
6.25 1.258 1.219 1.239 1.151
3.12 0.734 0.715 0.725 0.637
1.56 0.468 0.479 0.474 0.386
0.78 0.242 0.248 0.245 0.157
0.39 0.152 0.159 0.156 0.068
0 0.088 0.087 0.088  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells (By similarity).
Gene References into Functions
  1. These findings suggest a novel immunoinhibitory function for LGALS3BP that might be important for immune evasion of tumor cells during cancer progression. PMID: 25320078
  2. Data give novel evidence that CyCAP regulates the post-translational modification of tissue transglutaminase through its colocalization with calnexin in endoplasmic reticulum. PMID: 20049854
  3. Cyclophilin C-associated protein is expressed in skin and during wound repair. It is regulated by IFNgamma and may function on immune defense in macrophages, lymphocytes, dermal fibroblasts and keratinocytes during wound healing. PMID: 16998803
  4. CyCAP-deficient mice spontaneously and after challenge with carcinogen develop significantly more colorectal mucosal hyperplasia, an early stage in murine colonic carcinogenesis PMID: 19627619
Subcellular Location Secreted, Secreted, extracellular space, extracellular matrix
Tissue Specificity Detected in embryo, liver, spleen, kidney, lung, heart, intestine, thymus and lymph node.
Database Links

KEGG: mmu:19039

STRING: 10090.ENSMUSP00000035579

UniGene: Mm.3152

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