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Mouse Galectin-3-binding protein(LGALS3BP) ELISA kit

Protocol
Code CSB-EL012888MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
lectin, galactoside-binding, soluble, 3 binding protein
Alternative Names
Lgals3bp ELISA Kit; Cycap ELISA Kit; MamaGalectin-3-binding protein ELISA Kit; Cyp-C-associated protein ELISA Kit; CyCAP ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; Protein MAMA ELISA Kit
Abbreviation
LGALS3BP
Uniprot No.
Q07797
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.39 ng/mL-25 ng/mL
Sensitivity
0.09 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1000 Average % 94
Range % 90-98
1:2000 Average % 102
Range % 96-107
1:4000 Average % 87
Range % 81-92
1:8000 Average % 92
Range % 88-96
Recovery
The recovery of mouse LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 97-107
EDTA plasma (n=4) 98 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
25 2.867 2.723 2.795 2.707
12.5 1.988 1.856 1.922 1.834
6.25 1.258 1.219 1.239 1.151
3.12 0.734 0.715 0.725 0.637
1.56 0.468 0.479 0.474 0.386
0.78 0.242 0.248 0.245 0.157
0.39 0.152 0.159 0.156 0.068
0 0.088 0.087 0.088 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse LGALS3BP ELISA Kit was designed for the quantitative measurement of Mouse LGALS3BP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.39 ng/mL-25 ng/mL and the sensitivity is 0.09 ng/mL.

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Target Background

Function
(From Uniprot)
Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Gene References into Functions
  1. These findings suggest a novel immunoinhibitory function for LGALS3BP that might be important for immune evasion of tumor cells during cancer progression. PMID: 25320078
  2. Data give novel evidence that CyCAP regulates the post-translational modification of tissue transglutaminase through its colocalization with calnexin in endoplasmic reticulum. PMID: 20049854
  3. Cyclophilin C-associated protein is expressed in skin and during wound repair. It is regulated by IFNgamma and may function on immune defense in macrophages, lymphocytes, dermal fibroblasts and keratinocytes during wound healing. PMID: 16998803
  4. CyCAP-deficient mice spontaneously and after challenge with carcinogen develop significantly more colorectal mucosal hyperplasia, an early stage in murine colonic carcinogenesis PMID: 19627619
Subcellular Location
Secreted. Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Detected in embryo, liver, spleen, kidney, lung, heart, intestine, thymus and lymph node.
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