Mouse Group XIIA secretory phospholipase A2(PLA2G12A) ELISA kit

Code CSB-EL018086MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
phospholipase A2, group XIIA
Alternative Names
Pla2g12a ELISA Kit; Pla2g12 ELISA Kit; Group XIIA secretory phospholipase A2 ELISA Kit; GXII sPLA2 ELISA Kit; sPLA2-XII ELISA Kit; EC 3.1.1.4 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 12A ELISA Kit
Abbreviation
PLA2G12A
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %8-89
1:2Average %98
Range %94-103
1:4Average %85
Range %82-89
1:8Average %98
Range %94-102
Recovery
The recovery of mouse PLA2G12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8683-89
EDTA plasma (n=4)9087-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.410 2.324 2.367 2.178
20001.610 1.684 1.647 1.458
10001.085 1.034 1.060 0.871
5000.700 0.732 0.716 0.527
2500.468 0.478 0.473 0.284
1250.388 0.398 0.393 0.204
62.50.260 0.259 0.260 0.071
00.191 0.187 0.189
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PLA2G12A ELISA Kit was designed for the quantitative measurement of Mouse PLA2G12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.
Subcellular Location
Secreted. Cytoplasm.
Protein Families
Phospholipase A2 family
Database Links
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