Mouse Heat Shock Protein 90,Hsp-90 ELISA Kit

Code CSB-E08312m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07901
Lead Time 3-5 working days
Abbreviation HSP90AA1
Protein Biological Process 1 Neurobiology
Target Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Alias FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, a
Species Mus musculus (Mouse)
Protein Biological Process 3 Stress response
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hsp-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 93  
Range % 81-101  
1:10 Average % 95  
Range % 86-104  
1:20 Average % 95  
Range % 82-105  
1:40 Average % 93  
Range % 82-101  
Recovery
The recovery of mouse Hsp-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-105  
EDTA plasma (n=4) 95 89-110  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.773 2.787 2.780 2.665  
5 1.842 1.740 1.791 1.676  
2.5 1.105 1.164 1.135 1.020  
1.25 0.668 0.673 0.671 0.556  
0.625 0.398 0.403 0.401 0.286  
0.312 0.282 0.266 0.274 0.159  
0.156 0.159 0.152 0.156 0.041  
0 0.112 0.118 0.115    
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation.
Subcellular Location Nucleus, Cytoplasm, Melanosome, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

KEGG: mmu:15519

STRING: 10090.ENSMUSP00000021698

UniGene: Mm.1843

Pathway Estrogen signaling pathway
PI3K-Akt signaling pathway
Protein processing in endoplasmic reticulum
Necroptosis
Antigen processing and presentation
IL-17 signaling pathway
NOD-like receptor signaling pathway
Th17 cell differentiation

Related Products

Hsp90aa1 Proteins

Hsp90aa1 Proteins for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.