Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

Code CSB-E17417m
Size 96T,5×96T,10×96T
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Product Details

Target Name
Integrin alpha-M (Itgam/CD11b)
Alternative Names
Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
Abbreviation
Itgam/CD11b
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 86
Range % 81-90
1:200 Average % 103
Range % 99-108
1:400 Average % 97
Range % 92-101
1:800 Average % 90
Range % 86-94
Recovery
The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.603 2.527 2.565 2.376
500 1.920 1.865 1.893 1.704
250 1.367 1.441 1.404 1.215
125 0.905 0.928 0.917 0.728
62.5 0.586 0.574 0.580 0.391
31.2 0.439 0.419 0.429 0.240
15.6 0.291 0.304 0.298 0.109
0 0.186 0.192 0.189  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse Itgam/CD11b ELISA Kit was designed for the quantitative measurement of Mouse Itgam/CD11b protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
Protein Families
Integrin alpha chain family
Tissue Specificity
Predominantly expressed in monocytes and granulocytes. Expressed in a subset of peritoneal mast cells. Expressed in microglia (at protein level).
Database Links
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