Mouse Interleukin 31(IL-31) ELISA Kit

Product Details

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Target Background

Product Details

Target Name

interleukin 31

Alternative Names

Il31Interleukin-31 ELISA Kit; IL-31 ELISA Kit

Abbreviation

IL31

Uniprot No.

Q6EAL8

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-99
1:2	Average %	97
	Range %	87-105
1:4	Average %	95
	Range %	88-100
1:8	Average %	90
	Range %	82-98

Recovery

The recovery of mouse IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-104
EDTA plasma (n=4)	99	90-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.886	2.846	2.866	2.702
1000	2.347	2.336	2.342	2.178
500	1.559	1.602	1.581	1.417
250	0.897	0.878	0.888	0.724
125	0.532	0.557	0.545	0.381
62.5	0.366	0.374	0.370	0.206
31.25	0.262	0.245	0.254	0.090
0	0.162	0.165	0.164

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse IL31 ELISA Kit was designed for the quantitative measurement of Mouse IL31 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

Target Background

Function
(From Uniprot)

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.

Gene References into Functions

The serum levels of IL-33 and IL-31 were significantly up-regulated in both AR and allergic asthma patients compared with normal individuals. PMID: 30301503
EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction in CD4-positive T cells. PMID: 28067314
IL-31 increased the concentration of intracellular Ca(2+) ions in mouse keratinocytes. PMID: 29477033
IL-31 elicits itch not only through direct action on primary sensory neurones, but also by inducing LTB4 production in keratinocytes. PMID: 28512667
IL31 and its receptor, IL31RA, are highly expressed in various human and mouse cancer cell lines, as well as in tumor specimens from cancer patients. MC38 murine colon carcinoma cells depleted of IL31 exhibit an increase in invasive and migratory properties in vitro. PMID: 28147314
Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. PMID: 26198770
IL-31 upregulates IL-31RA expression in DRG neuron cell bodies, and cutaneous-injected IL-31-induced itching is enhanced by DRG IL-31RA expression in mice PMID: 25381841
our results suggest a functional cooperation between NFAT1 and JunB in mediating IL-31 gene expression in CD4(+) T cells PMID: 25595785
Data not only illustrate various functions that cysteines perform during IL-31 biosynthesis and secretion, but also highlight their potential roles in cytokine effector functions. PMID: 21182835
IL-31 may be involved in promoting the dermatitis and epithelial responses that characterize allergic and non-allergic diseases PMID: 15184896
IL-31 interaction with IL-31 receptor (IL-31R) is identified in an acute model of T helper cell type 2 inflammation in the lung. PMID: 17353366
-31 antibody reduces scratching behaviour in an atopic dermatitis-like murine model during the onset of clinical skin manifestations PMID: 19054054
these data provide the first evidence of a function for IL-31-IL-31R interactions in limiting the magnitude of type 2 inflammatory responses within the intestine. PMID: 19414760

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Subcellular Location

Secreted.

Database Links

KEGG: mmu:76399

STRING: 10090.ENSMUSP00000031389

UniGene: Mm.75049

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Code	CSB-E13660m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.